Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
02 06 2022
02 06 2022
Historique:
received:
14
12
2021
accepted:
27
04
2022
entrez:
2
6
2022
pubmed:
3
6
2022
medline:
7
6
2022
Statut:
epublish
Résumé
Cell-cultured meat offers the potential for a more sustainable, ethical, resilient, and healthy food system. However, research and development has been hindered by the lack of serum-free media that enable the robust expansion of relevant cells (e.g., muscle satellite cells) over multiple passages. Recently, a low-cost serum-free media (B8) was described for pluripotent stem cells. Here, B8 is adapted for bovine satellite cells through the addition of a single component, recombinant albumin, which renders it suitable for long-term satellite cell expansion without sacrificing myogenicity. This new media (Beefy-9) maintains cell growth over the entire period tested (seven passages), with an average doubling time of 39 h. Along with demonstrated efficacy for bovine cells, Beefy-9 offers a promising starting-point for developing serum-free media for other meat-relevant species. Ultimately, this work offers a foundation for escaping cultured meat research's reliance on serum, thereby accelerating the field.
Identifiants
pubmed: 35654948
doi: 10.1038/s42003-022-03423-8
pii: 10.1038/s42003-022-03423-8
pmc: PMC9163123
doi:
Substances chimiques
Culture Media, Serum-Free
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
466Subventions
Organisme : NIBIB NIH HHS
ID : P41 EB002520
Pays : United States
Organisme : NIH HHS
ID : S10 OD021624
Pays : United States
Informations de copyright
© 2022. The Author(s).
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