Shift of Neutrophils From Blood to Bone Marrow Upon Extensive Experimental Trauma Surgery.
bone marrow
neutrophil
porcine model
subsets
trauma
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2022
2022
Historique:
received:
25
02
2022
accepted:
29
03
2022
entrez:
3
6
2022
pubmed:
4
6
2022
medline:
7
6
2022
Statut:
epublish
Résumé
Extensive trauma surgery evokes an immediate cellular immune response including altered circulatory neutrophil numbers. The concurrent bone marrow (BM) response however is currently unclear. We hypothesize that these BM changes include (1) a relative reduction of the bone marrow neutrophil fraction and (2) increasing heterogeneity of the bone marrow neutrophil pool due to (3) the appearance of aged/returning neutrophils from circulation into the BM-compartment. Eight pigs were included in a standardized extensive trauma surgery model. Blood and bone marrow samples were collected at baseline and after 3 hours of ongoing trauma surgery. Leukocyte and subtype counts and cell surface receptor expression levels were studied by flow cytometry. All animals survived the interventions. A significant drop in circulating neutrophil counts from 9.3 to 3.2x10 The current study shows a shift in composition of the BM neutrophil pool during extensive trauma surgery that was associated with a relatively circulatory neutropenia. More specifically, under these conditions BM neutrophils were more mature than under homeostatic conditions and a CXCR4
Identifiants
pubmed: 35655784
doi: 10.3389/fimmu.2022.883863
pmc: PMC9152003
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
883863Informations de copyright
Copyright © 2022 Teuben, Heeres, Blokhuis, Spijkerman, Knot, Vrisekoop, Pfeifer, Pape, Koenderman and Leenen.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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