Novel in vitro methods for simultaneous screening of two antagonistic bacteria against multiple fungal phytopathogens in a single agar plate.
Bacterial antagonists
Biological control
Culture plate assay
Modified in vitro screening
Multiple fungal phytopathogens
Journal
3 Biotech
ISSN: 2190-572X
Titre abrégé: 3 Biotech
Pays: Germany
ID NLM: 101565857
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
received:
29
09
2021
accepted:
12
05
2022
entrez:
3
6
2022
pubmed:
4
6
2022
medline:
4
6
2022
Statut:
ppublish
Résumé
Two novel and rapid in vitro agar plate methods for simultaneous screening of two bacterial isolates for antagonism against multiple fungal phytopathogens in a single agar plate are described. In the modified plus streak method (MPS), in a 10 cm diameter PDA agar plate, two different bacterial isolates were screened against four fungal pathogens. The second method used 20 cm diameter PDA agar plate surface and was termed as the parallel multiple streak (PMS) method. Here, two bacterial isolates were screened for antagonism against ten fungal phytopathogens on a single agar plate surface. Our new methods are rapid and more economical, and could reduce the number of agar plates and the medium required for in vitro screening by several fold, compared to that used in the conventional dual culture plate assay.
Identifiants
pubmed: 35656385
doi: 10.1007/s13205-022-03205-3
pii: 3205
pmc: PMC9151952
doi:
Types de publication
Journal Article
Langues
eng
Pagination
140Informations de copyright
© King Abdulaziz City for Science and Technology 2022.
Déclaration de conflit d'intérêts
Conflict of interestThe authors declare that they have no conflict of interest.
Références
Nat Rev Microbiol. 2005 Apr;3(4):307-19
pubmed: 15759041
Science. 1982 Jun 25;216(4553):1376-81
pubmed: 17798345
Lett Appl Microbiol. 2021 Aug;73(2):229-236
pubmed: 33948964
Annu Rev Phytopathol. 1998;36:453-83
pubmed: 15012509
Microbiol Res. 2020 Feb;232:126389
pubmed: 31821969
Microbes Environ. 2017 Jun 24;32(2):133-141
pubmed: 28515390
Microbiol Res. 2003;158(2):91-7
pubmed: 12906381
FEMS Microbiol Rev. 2010 Nov;34(6):1037-62
pubmed: 20412310
Plant Dis. 1998 Sep;82(9):1022-1028
pubmed: 30856829
Genet Mol Biol. 2012 Dec;35(4 (suppl)):1044-51
pubmed: 23411488