ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling.


Journal

The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356

Informations de publication

Date de publication:
04 07 2022
Historique:
received: 08 06 2021
accepted: 03 05 2022
entrez: 3 6 2022
pubmed: 4 6 2022
medline: 9 6 2022
Statut: ppublish

Résumé

Mutations in VPS13C cause early-onset, autosomal recessive Parkinson's disease (PD). We have established that VPS13C encodes a lipid transfer protein localized to contact sites between the ER and late endosomes/lysosomes. In the current study, we demonstrate that depleting VPS13C in HeLa cells causes an accumulation of lysosomes with an altered lipid profile, including an accumulation of di-22:6-BMP, a biomarker of the PD-associated leucine-rich repeat kinase 2 (LRRK2) G2019S mutation. In addition, the DNA-sensing cGAS-STING pathway, which was recently implicated in PD pathogenesis, is activated in these cells. This activation results from a combination of elevated mitochondrial DNA in the cytosol and a defect in the degradation of activated STING, a lysosome-dependent process. These results suggest a link between ER-lysosome lipid transfer and innate immune activation in a model human cell line and place VPS13C in pathways relevant to PD pathogenesis.

Identifiants

pubmed: 35657605
pii: 213259
doi: 10.1083/jcb.202106046
pmc: PMC9170524
pii:
doi:

Substances chimiques

DNA, Mitochondrial 0
Lipids 0
Membrane Proteins 0
Proteins 0
STING1 protein, human 0
VPS13C protein, human 0
LRRK2 protein, human EC 2.7.11.1
Leucine-Rich Repeat Serine-Threonine Protein Kinase-2 EC 2.7.11.1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Chan Zuckerberg Initiative
ID : 2020-221912
Organisme : Michael J. Fox Foundation for Parkinson's Research
ID : ASAP-000580
Organisme : China Scholarship Counsel
Organisme : Aligning Science Across Parkinson's
Organisme : American Heart Association
Organisme : NIDA NIH HHS
ID : P30 DA018343
Pays : United States
Organisme : Parkinson Foundation
ID : PF-RCE-1946
Organisme : NIDDK NIH HHS
ID : P30 DK045735
Pays : United States
Organisme : Audrey Geisel Chair in Biomedical Science
Organisme : NIH HHS
ID : NS36251
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR069876
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007205
Pays : United States
Organisme : Allen Initiative in Brain Health and Cognitive Impairment award
ID : 19PABH134610000

Informations de copyright

© 2022 Hancock-Cerutti et al.

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Auteurs

William Hancock-Cerutti (W)

Department of Neuroscience, Yale University School of Medicine, New Haven, CT.
Department of Cell Biology, Yale University School of Medicine, New Haven, CT.
Interdepartmental Neuroscience Program, Yale School of Medicine, New Haven, CT.
MD/PhD Program, Yale School of Medicine, New Haven, CT.
Howard Hughes Medical Institute, Chevy Chase, MD.
Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT.
Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD.

Zheng Wu (Z)

Department of Genetics, Yale School of Medicine, New Haven, CT.
Salk Institute for Biological Studies, La Jolla, CA.

Peng Xu (P)

Department of Neuroscience, Yale University School of Medicine, New Haven, CT.
Department of Cell Biology, Yale University School of Medicine, New Haven, CT.
Howard Hughes Medical Institute, Chevy Chase, MD.
Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT.
Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD.

Narayana Yadavalli (N)

Department of Cell Biology, Yale University School of Medicine, New Haven, CT.
Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD.

Marianna Leonzino (M)

Department of Neuroscience, Yale University School of Medicine, New Haven, CT.
Department of Cell Biology, Yale University School of Medicine, New Haven, CT.
Howard Hughes Medical Institute, Chevy Chase, MD.
Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT.
Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD.

Arun Kumar Tharkeshwar (AK)

Department of Cell Biology, Yale University School of Medicine, New Haven, CT.

Shawn M Ferguson (SM)

Department of Neuroscience, Yale University School of Medicine, New Haven, CT.
Department of Cell Biology, Yale University School of Medicine, New Haven, CT.
Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT.
Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD.

Gerald S Shadel (GS)

Salk Institute for Biological Studies, La Jolla, CA.

Pietro De Camilli (P)

Department of Neuroscience, Yale University School of Medicine, New Haven, CT.
Department of Cell Biology, Yale University School of Medicine, New Haven, CT.
Howard Hughes Medical Institute, Chevy Chase, MD.
Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT.
Kavli Institute for Neuroscience, Yale University School of Medicine, New Haven, CT.
Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD.

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