Tet2 deficiency drives liver microbiome dysbiosis triggering Tc1 cell autoimmune hepatitis.


Journal

Cell host & microbe
ISSN: 1934-6069
Titre abrégé: Cell Host Microbe
Pays: United States
ID NLM: 101302316

Informations de publication

Date de publication:
13 07 2022
Historique:
received: 11 10 2021
revised: 04 03 2022
accepted: 09 05 2022
pubmed: 7 6 2022
medline: 19 7 2022
entrez: 6 6 2022
Statut: ppublish

Résumé

The triggers that drive interferon-γ (IFNγ)-producing CD8 T cell (Tc1 cell)-mediated autoimmune hepatitis (AIH) remain obscure. Here, we show that lack of hematopoietic Tet methylcytosine dioxygenase 2 (Tet2), an epigenetic regulator associated with autoimmunity, results in the development of microbiota-dependent AIH-like pathology, accompanied by hepatic enrichment of aryl hydrocarbon receptor (AhR) ligand-producing pathobionts and rampant Tc1 cell immunity. We report that AIH-like disease development is dependent on both IFNγ and AhR signaling, as blocking either reverts ongoing AIH-like pathology. Illustrating the critical role of AhR-ligand-producing pathobionts in this condition, hepatic translocation of the AhR ligand indole-3-aldehyde (I3A)-releasing Lactobacillus reuteri is sufficient to trigger AIH-like pathology. Finally, we demonstrate that I3A is required for L. reuteri-induced Tc1 cell differentiation in vitro and AIH-like pathology in vivo, both of which are restrained by Tet2 within CD8 T cells. This AIH-disease model may contribute to the development of therapeutics to alleviate AIH.

Identifiants

pubmed: 35658976
pii: S1931-3128(22)00266-9
doi: 10.1016/j.chom.2022.05.006
pmc: PMC9841318
mid: NIHMS1858316
pii:
doi:

Substances chimiques

DNA-Binding Proteins 0
Ligands 0
Interferon-gamma 82115-62-6
Dioxygenases EC 1.13.11.-
Tet2 protein, mouse EC 1.13.11.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1003-1019.e10

Subventions

Organisme : NIAID NIH HHS
ID : R21 AI163721
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA253329
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA047904
Pays : United States
Organisme : NCI NIH HHS
ID : R21 CA259636
Pays : United States
Organisme : NIH HHS
ID : S10 OD023402
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK120531
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007281
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI089443
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK130897
Pays : United States
Organisme : NIAMS NIH HHS
ID : K08 AR075056
Pays : United States
Organisme : NIAAA NIH HHS
ID : R01 AA021978
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI163503
Pays : United States

Informations de copyright

Copyright © 2022 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

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Auteurs

Surya P Pandey (SP)

Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Mackenzie J Bender (MJ)

Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Alex C McPherson (AC)

Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA; Department of Infectious Diseases and Microbiology, University of Pittsburgh School of Public Health, Pittsburgh, PA, USA.

Catherine M Phelps (CM)

Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Luzmariel Medina Sanchez (LM)

Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Mohit Rana (M)

Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Lee Hedden (L)

Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Kishan A Sangani (KA)

Department of Medicine, University of Chicago, Chicago, IL, USA; Committee on Immunology, University of Chicago, Chicago, IL, USA.

Li Chen (L)

Department of Medicine, University of Chicago, Chicago, IL, USA; Committee on Immunology, University of Chicago, Chicago, IL, USA.

Jake H Shapira (JH)

Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Magdalena Siller (M)

Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Chhavi Goel (C)

Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Elena F Verdú (EF)

Division of Gastroenterology, Department of Internal Medicine, Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON, Canada.

Bana Jabri (B)

Department of Medicine, University of Chicago, Chicago, IL, USA; Committee on Immunology, University of Chicago, Chicago, IL, USA; Department of Pathology, University of Chicago, Chicago, IL, USA; Department of Pediatrics, University of Chicago, Chicago, IL, USA.

Alexander Chang (A)

Department of Biomedical Informatics, University of Pittsburgh, Pittsburgh, PA, USA.

Uma R Chandran (UR)

Department of Biomedical Informatics, University of Pittsburgh, Pittsburgh, PA, USA.

Steven J Mullett (SJ)

Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA; Health Sciences Metabolomics and Lipidomics Core, University of Pittsburgh, Pittsburgh, PA, USA.

Stacy G Wendell (SG)

Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA; Health Sciences Metabolomics and Lipidomics Core, University of Pittsburgh, Pittsburgh, PA, USA.

Aatur D Singhi (AD)

Department of Pathology, University of Pittsburgh, Pittsburgh, PA, USA.

Jeremy S Tilstra (JS)

Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Joseph F Pierre (JF)

Department of Nutritional Sciences, University of Wisconsin-Madison, Madison, WI, USA.

Gavin E Arteel (GE)

Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA, USA.

Reinhard Hinterleitner (R)

Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Marlies Meisel (M)

Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. Electronic address: marlies@pitt.edu.

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