Humanized yeast to model human biology, disease and evolution.
Functional complementation
Functional replaceability
Humanized yeast
Orthology
Journal
Disease models & mechanisms
ISSN: 1754-8411
Titre abrégé: Dis Model Mech
Pays: England
ID NLM: 101483332
Informations de publication
Date de publication:
01 06 2022
01 06 2022
Historique:
entrez:
6
6
2022
pubmed:
7
6
2022
medline:
9
6
2022
Statut:
ppublish
Résumé
For decades, budding yeast, a single-cellular eukaryote, has provided remarkable insights into human biology. Yeast and humans share several thousand genes despite morphological and cellular differences and over a billion years of separate evolution. These genes encode critical cellular processes, the failure of which in humans results in disease. Although recent developments in genome engineering of mammalian cells permit genetic assays in human cell lines, there is still a need to develop biological reagents to study human disease variants in a high-throughput manner. Many protein-coding human genes can successfully substitute for their yeast equivalents and sustain yeast growth, thus opening up doors for developing direct assays of human gene function in a tractable system referred to as 'humanized yeast'. Humanized yeast permits the discovery of new human biology by measuring human protein activity in a simplified organismal context. This Review summarizes recent developments showing how humanized yeast can directly assay human gene function and explore variant effects at scale. Thus, by extending the 'awesome power of yeast genetics' to study human biology, humanizing yeast reinforces the high relevance of evolutionarily distant model organisms to explore human gene evolution, function and disease.
Identifiants
pubmed: 35661208
pii: 275614
doi: 10.1242/dmm.049309
pmc: PMC9194483
pii:
doi:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Review
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2022. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests The authors declare no competing or financial interests.
Références
Ned Tijdschr Geneeskd. 2008 Mar 1;152(9):518-9
pubmed: 18389888
Genome Res. 2003 Nov;13(11):2498-504
pubmed: 14597658
Nat Biotechnol. 2003 Sep;21(9):1075-81
pubmed: 12910243
Genetics. 2020 Dec;216(4):995-1007
pubmed: 33037049
Science. 2017 Mar 10;355(6329):1040-1044
pubmed: 28280199
Science. 2003 Dec 5;302(5651):1772-5
pubmed: 14657500
Genome Res. 2009 Dec;19(12):2324-33
pubmed: 19767417
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D319-22
pubmed: 14681423
Mol Syst Biol. 2017 Dec 21;13(12):957
pubmed: 29269382
Nat Biotechnol. 2011 Apr;29(4):361-7
pubmed: 21441928
Database (Oxford). 2012 Mar 20;2012:bar062
pubmed: 22434830
Genome Biol. 2004;5(7):229
pubmed: 15239820
Proc Natl Acad Sci U S A. 2008 Jun 10;105(23):8055-60
pubmed: 18523009
Elife. 2017 Jun 29;6:
pubmed: 28661399
Nat Methods. 2010 Sep;7(9):741-6
pubmed: 20711194
Cell. 1998 Dec 23;95(7):981-91
pubmed: 9875852
J Microbiol. 2009 Dec;47(6):789-95
pubmed: 20127475
J Inherit Metab Dis. 2020 Jul;43(4):758-769
pubmed: 32077105
Curr Opin Microbiol. 2018 Oct;45:170-179
pubmed: 30059827
J Biol Chem. 2007 Apr 13;282(15):10853-64
pubmed: 17261589
Nat Commun. 2018 Nov 9;9(1):4737
pubmed: 30413698
Elife. 2015 Jul 23;4:e06322
pubmed: 26202976
EMBO Rep. 2008 Aug;9(8):717-20
pubmed: 18670440
Genome Med. 2020 Jan 30;12(1):13
pubmed: 32000841
Proc Natl Acad Sci U S A. 2010 Apr 6;107(14):6544-9
pubmed: 20308572
Genomics. 2007 Mar;89(3):307-15
pubmed: 17207965
Science. 2008 Apr 18;320(5874):362-5
pubmed: 18420932
FEMS Yeast Res. 2016 Dec;16(8):
pubmed: 27770011
Mol Syst Biol. 2016 Apr 22;12(4):865
pubmed: 27107014
Gene. 1998 Nov 26;223(1-2):9-20
pubmed: 9858667
Science. 2015 May 22;348(6237):921-5
pubmed: 25999509
Genetics. 2021 Aug 26;219(1):
pubmed: 34849907
EMBO J. 1992 Dec;11(13):5111-20
pubmed: 1361173
Nucleic Acids Res. 2015 Jan;43(Database issue):D789-98
pubmed: 25428349
Genetics. 2020 Mar;214(3):735-747
pubmed: 31937519
Genetics. 2014 Oct;198(2):435-7
pubmed: 25316779
Nat Commun. 2019 Sep 13;10(1):4162
pubmed: 31519910
Cell. 2017 Dec 14;171(7):1508-1519.e13
pubmed: 29198523
Science. 2019 Sep 27;365(6460):1396-1400
pubmed: 31604265
Cancer Res. 2001 May 15;61(10):4175-83
pubmed: 11358842
Genetics. 2011 Nov;189(3):695-704
pubmed: 22084421
Nucleic Acids Res. 2020 Jan 10;48(1):486-499
pubmed: 31745563
Cell. 2013 Sep 26;155(1):27-38
pubmed: 24074859
Science. 1988 Jun 10;240(4858):1439-43
pubmed: 3287619
Plasmid. 2013 Jul;70(1):2-17
pubmed: 23541845
Curr Opin Genet Dev. 2015 Dec;35:16-24
pubmed: 26338499
PLoS One. 2007 Aug 22;2(8):e766
pubmed: 17712414
Nat Rev Genet. 2013 May;14(5):360-6
pubmed: 23552219
PLoS Biol. 2020 May 18;18(5):e3000627
pubmed: 32421706
Hum Mol Genet. 2009 Aug 1;18(15):2889-98
pubmed: 19454486
Curr Opin Syst Biol. 2017 Dec;6:14-21
pubmed: 30505984
ACS Synth Biol. 2015 Sep 18;4(9):975-86
pubmed: 25871405
Nature. 2016 Aug 17;536(7616):285-91
pubmed: 27535533
Biosci Rep. 2004 Aug-Oct;24(4-5):523-44
pubmed: 16134024
Mol Cell. 2004 Nov 5;16(3):487-96
pubmed: 15525520
Nat Genet. 2005 May;37(5):526-31
pubmed: 15806102
BMC Bioinformatics. 2013 Jun 21;14:203
pubmed: 23800157
Annu Rev Genet. 2007;41:465-507
pubmed: 17678444
Nature. 2021 Aug;596(7873):583-589
pubmed: 34265844
Cell. 2019 Mar 21;177(1):85-100
pubmed: 30901552
Nat Methods. 2011 Jun 26;8(8):659-61
pubmed: 21706014
Stud Hist Philos Biol Biomed Sci. 2012 Mar;43(1):29-36
pubmed: 22326070
J Biomol Screen. 2010 Apr;15(4):368-78
pubmed: 20237203
Cell. 2016 Jan 14;164(1-2):310-323
pubmed: 26771498
N Engl J Med. 1984 Mar 1;310(9):550-3
pubmed: 6694706
Neoplasma. 1999;46(2):80-8
pubmed: 10466430
Mol Pharmacol. 2006 Oct;70(4):1395-405
pubmed: 16847144
PLoS Genet. 2017 May 25;13(5):e1006779
pubmed: 28542158
Gene. 1983 Aug;23(2):221-32
pubmed: 6352414
Mutat Res. 2004 May;566(3):231-48
pubmed: 15082239
Science. 2016 Sep 23;353(6306):
pubmed: 27708008
Nat Commun. 2014 Dec 18;5:5585
pubmed: 25519239
Science. 2018 Apr 20;360(6386):
pubmed: 29674565
Development. 2021 Oct 1;148(19):
pubmed: 35231122
Nature. 1987 May 7-13;327(6117):31-5
pubmed: 3553962
Genetics. 2020 Aug;215(4):1153-1169
pubmed: 32522745
Nat Protoc. 2014 Sep;9(9):2267-84
pubmed: 25167058
Nat Rev Genet. 2002 Nov;3(11):838-49
pubmed: 12415314
Nature. 2002 Jul 25;418(6896):387-91
pubmed: 12140549
Nat Biotechnol. 2010 Jun;28(6):617-623
pubmed: 20473289
Nat Rev Genet. 2001 Apr;2(4):302-12
pubmed: 11283702
Genetics. 2015 Nov;201(3):1263-74
pubmed: 26354769
Genome Res. 2017 Sep;27(9):1487-1500
pubmed: 28596290
Proc Natl Acad Sci U S A. 2008 May 13;105(19):6987-92
pubmed: 18458337
PLoS Genet. 2010 May 27;6(5):e1000968
pubmed: 20523748
Haematologica. 2019 Dec 19;105(10):2391-2399
pubmed: 33054079
Nucleic Acids Res. 2015 Jan;43(Database issue):D234-9
pubmed: 25429972
Brief Funct Genomics. 2016 Mar;15(2):155-63
pubmed: 26462863
Hum Mol Genet. 1994;3 Spec No:1509-17
pubmed: 7849746
Bioinformatics. 2020 Jun 1;36(12):3938-3940
pubmed: 32251504
Genetics. 2014 May;197(1):33-48
pubmed: 24807111
Nature. 2015 Oct 1;526(7571):68-74
pubmed: 26432245
Mol Biol Cell. 2014 Mar;25(5):549-53
pubmed: 24577733
Science. 1999 Aug 6;285(5429):901-6
pubmed: 10436161
Cold Spring Harb Protoc. 2015 Aug 03;2015(8):711-4
pubmed: 26240414
Nucleic Acids Res. 2020 Jan 8;48(D1):D650-D658
pubmed: 31552413
Genetics. 2015 Oct;201(2):403-23
pubmed: 26447128
Cold Spring Harb Protoc. 2015 Dec 02;2015(12):pdb.top083840
pubmed: 26631132
Science. 2010 Dec 24;330(6012):1724
pubmed: 21177975
Genetics. 2019 Dec;213(4):1189-1196
pubmed: 31796553
Nucleic Acids Res. 2021 Jan 8;49(D1):D480-D489
pubmed: 33237286
Nucleic Acids Res. 2013 Apr;41(7):4336-43
pubmed: 23460208
Hum Mol Genet. 2019 Nov 21;28(R2):R207-R214
pubmed: 31227826
Proc Natl Acad Sci U S A. 2008 Oct 28;105(43):16653-8
pubmed: 18931302
Proc Natl Acad Sci U S A. 2011 Jul 19;108(29):11989-94
pubmed: 21715656
Genome Res. 2016 May;26(5):670-80
pubmed: 26975778
Nucleic Acids Res. 2002 Jan 1;30(1):69-72
pubmed: 11752257
Genetics. 2012 Mar;190(3):841-54
pubmed: 22419077
Genetics. 2021 Oct 2;219(2):
pubmed: 34849890
Am J Hum Genet. 2021 Sep 2;108(9):1735-1751
pubmed: 34314704
Chembiochem. 2002 Jul 2;3(7):596-603
pubmed: 12324993
Annu Rev Biochem. 2013;82:415-45
pubmed: 23495936
Mol Reprod Dev. 2017 Nov;84(11):1118-1132
pubmed: 28834577
Cell. 2004 Jul 9;118(1):31-44
pubmed: 15242642
Bio Protoc. 2018 Mar 20;8(6):
pubmed: 29770349
Mol Biol Evol. 2019 Feb 1;36(2):304-314
pubmed: 30428072
Nat Rev Genet. 2008 Nov;9(11):855-67
pubmed: 18852697
Nat Genet. 1993 Oct;5(2):124-9
pubmed: 8252037