Longitudinal SARS-CoV-2 RNA wastewater monitoring across a range of scales correlates with total and regional COVID-19 burden in a well-defined urban population.
COVID-19
Census data
Epidemiological monitoring
Neighborhoods
RT-qPCR
Wastewater-based epidemiology
Journal
Water research
ISSN: 1879-2448
Titre abrégé: Water Res
Pays: England
ID NLM: 0105072
Informations de publication
Date de publication:
15 Jul 2022
15 Jul 2022
Historique:
received:
18
11
2021
revised:
13
04
2022
accepted:
13
05
2022
pubmed:
7
6
2022
medline:
22
6
2022
entrez:
6
6
2022
Statut:
ppublish
Résumé
Wastewater-based epidemiology (WBE) is an emerging surveillance tool that has been used to monitor the ongoing COVID-19 pandemic by tracking SARS-CoV-2 RNA shed into wastewater. WBE was performed to monitor the occurrence and spread of SARS-CoV-2 from three wastewater treatment plants (WWTP) and six neighborhoods in the city of Calgary, Canada (population 1.44 million). A total of 222 WWTP and 192 neighborhood samples were collected from June 2020 to May 2021, encompassing the end of the first-wave (June 2020), the second-wave (November end to December 2020) and the third-wave of the COVID-19 pandemic (mid-April to May 2021). Flow-weighted 24-hour composite samples were processed to extract RNA that was then analyzed for two SARS-CoV-2-specific regions of the nucleocapsid gene, N1 and N2, using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Using this approach SARS-CoV-2 RNA was detected in 98.06% (406/414) of wastewater samples. SARS-CoV-2 RNA abundance was compared to clinically diagnosed COVID-19 cases organized by the three-digit postal code of affected individuals' primary residences, enabling correlation analysis at neighborhood, WWTP and city-wide scales. Strong correlations were observed between N1 & N2 gene signals in wastewater and new daily cases for WWTPs and neighborhoods. Similarly, when flow rates at Calgary's three WWTPs were used to normalize observed concentrations of SARS-CoV-2 RNA and combine them into a city-wide signal, this was strongly correlated with regionally diagnosed COVID-19 cases and clinical test percent positivity rate. Linked census data demonstrated disproportionate SARS-CoV-2 in wastewater from areas of the city with lower socioeconomic status and more racialized communities. WBE across a range of urban scales was demonstrated to be an effective mechanism of COVID-19 surveillance.
Identifiants
pubmed: 35661506
pii: S0043-1354(22)00564-4
doi: 10.1016/j.watres.2022.118611
pmc: PMC9107283
pii:
doi:
Substances chimiques
RNA, Viral
0
Waste Water
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
118611Informations de copyright
Copyright © 2022. Published by Elsevier Ltd.
Références
Sci Total Environ. 2021 Nov 20;796:148887
pubmed: 34274669
Int J Environ Res Public Health. 2021 Mar 11;18(6):
pubmed: 33799791
Science. 2021 May 28;372(6545):
pubmed: 33906968
N Engl J Med. 2021 Jul 8;385(2):97-100
pubmed: 33789007
J Public Health (Oxf). 2020 Nov 23;42(4):660-664
pubmed: 32657332
Health Rep. 2020 Jun 17;31(3):3-13
pubmed: 32644759
Euro Surveill. 2020 Dec;25(50):
pubmed: 33334397
Nat Biotechnol. 2020 Oct;38(10):1164-1167
pubmed: 32948856
Lancet Reg Health Eur. 2021 Nov;10:100202
pubmed: 34423327
Sci Total Environ. 2020 Dec 20;749:141364
pubmed: 32836117
Sci Total Environ. 2021 Sep 15;787:147463
pubmed: 33989864
Lancet Gastroenterol Hepatol. 2020 May;5(5):434-435
pubmed: 32199469
mSystems. 2020 Jul 21;5(4):
pubmed: 32694130
Pathogens. 2021 Aug 10;10(8):
pubmed: 34451472
Water Res. 2021 Aug 1;201:117369
pubmed: 34229222
Chemosphere. 2021 Nov;283:131194
pubmed: 34467943
Trop Dis Travel Med Vaccines. 2021 Jun 10;7(1):15
pubmed: 34112261
Emerg Infect Dis. 2020 Jun;26(6):1337-1339
pubmed: 32150527
Clin Infect Dis. 2020 Sep 12;71(6):1547-1551
pubmed: 32112072
Sci Total Environ. 2021 Dec 15;800:149480
pubmed: 34392211
Sci Total Environ. 2021 Jan 10;751:141750
pubmed: 32861187
J Public Health (Oxf). 2021 Jun 7;43(2):e307-e308
pubmed: 33559684
Sci Total Environ. 2020 Sep 20;736:139631
pubmed: 32474280
Emerg Infect Dis. 2021 Sep;27(9):1-8
pubmed: 34424162
Euro Surveill. 2021 Jul;26(28):
pubmed: 34269175
J Med Virol. 2020 Jun;92(6):680-682
pubmed: 32124995
Int J Environ Res Public Health. 2019 Jun 13;16(12):
pubmed: 31200573
Gastroenterology. 2020 Jul;159(1):81-95
pubmed: 32251668
JAMA Netw Open. 2021 Aug 2;4(8):e2122240
pubmed: 34448865
Sci Total Environ. 2020 Oct 15;739:139076
pubmed: 32758929
Environ Sci Technol. 2021 Apr 20;55(8):4880-4888
pubmed: 33759506
Sci Total Environ. 2020 Dec 1;746:141326
pubmed: 32768790
Int J Infect Dis. 2020 Jun;95:363-370
pubmed: 32335340
JAMA Netw Open. 2021 Jan 4;4(1):e2036462
pubmed: 33512520
Nat Hum Behav. 2020 Dec;4(12):1294-1302
pubmed: 33144713
Water Res. 2021 Sep 1;202:117388
pubmed: 34229195
Sci Total Environ. 2021 Dec 20;801:149757
pubmed: 34467932
Water Res. 2020 Aug 15;181:115942
pubmed: 32425251
Sci Total Environ. 2020 Sep 20;736:139652
pubmed: 32464333
Environ Res. 2021 Apr;195:110748
pubmed: 33465345
Public Health. 2020 Jun;183:110-111
pubmed: 32502699
Lancet Infect Dis. 2021 Sep;21(9):1246-1256
pubmed: 33857406
Cell Rep Med. 2020 Sep 22;1(6):100098
pubmed: 32904687
Lancet Digit Health. 2020 Sep;2(9):e441
pubmed: 32864599
Sci Total Environ. 2021 Nov 25;797:149031
pubmed: 34346361
Pathogens. 2021 Jun 23;10(7):
pubmed: 34201687
Sci Total Environ. 2020 Aug 1;728:138764
pubmed: 32387778
Sci Total Environ. 2021 May 1;767:145124
pubmed: 33548842
JAMA. 2021 Jun 22;325(24):2500-2502
pubmed: 33956050
Sci Total Environ. 2020 Oct 1;737:140405
pubmed: 32783878
Water Res. 2021 Sep 1;202:117400
pubmed: 34274898
Int J Infect Dis. 2021 Jul;108:296-299
pubmed: 33989774
Environ Sci Technol. 2021 Nov 16;55(22):15276-15286
pubmed: 34738785
Water Res. 2021 Jan 1;188:116560
pubmed: 33137526
Environ Sci Technol. 2021 Jul 6;55(13):8484-8491
pubmed: 34101444
Gut. 1992 Jun;33(6):818-24
pubmed: 1624166
Environ Sci Technol. 2020 Jul 7;54(13):7754-7757
pubmed: 32530639