Effect of ancient Khorasan wheat on gut microbiota, inflammation, and short-chain fatty acid production in patients with fibromyalgia.


Journal

World journal of gastroenterology
ISSN: 2219-2840
Titre abrégé: World J Gastroenterol
Pays: United States
ID NLM: 100883448

Informations de publication

Date de publication:
14 May 2022
Historique:
received: 26 11 2021
revised: 19 01 2022
accepted: 27 03 2022
entrez: 6 6 2022
pubmed: 7 6 2022
medline: 9 6 2022
Statut: ppublish

Résumé

Fibromyalgia (FM) syndrome is mainly characterized by widespread pain, sleeping disorders, fatigue, and cognitive dysfunction. In many cases, gastrointestinal distress is also reported, suggesting the potential pathogenic role of the gut microbiota (GM). The GM is deeply influenced by several environmental factors, especially the diet, and recent findings highlighted significant symptom improvement in FM patients following various nutritional interventions such as vegetarian diet, low-fermentable oligosaccharides, disaccharides, monosaccharides, and polyols based diets, gluten-free diet, and especially an ancient grain supplementation. In particular, a recent study reported that a replacement diet with ancient Khorasan wheat led to an overall improvement in symptom severity of FM patients. To examine the effects of ancient Khorasan wheat on the GM, inflammation, and short-chain fatty acid production in FM patients. After a 2-wk run-in period, 20 FM patients were enrolled in this randomized, double-blind crossover trial. In detail, they were assigned to consume either Khorasan or control wheat products for 8 wk and then, following an 8-wk washout period, crossed. Before and after treatments, GM characterization was performed by 16S rRNA sequencing while the fecal molecular inflammatory response and the short-chain fatty acids (SCFAs) were respectively determined with the Luminex MAGPIX detection system and a mass chromatography-mass spectrometry method. The Khorasan wheat replacement diet, in comparison with the control wheat diet, had more positive effects on intestinal microbiota composition and on both the fecal immune and SCFAs profiles such as the significant increase of butyric acid levels ( The replacement diet based on ancient Khorasan wheat results in beneficial GM compositional and functional modifications that positively correlate with an improvement of FM symptomatology.

Sections du résumé

BACKGROUND BACKGROUND
Fibromyalgia (FM) syndrome is mainly characterized by widespread pain, sleeping disorders, fatigue, and cognitive dysfunction. In many cases, gastrointestinal distress is also reported, suggesting the potential pathogenic role of the gut microbiota (GM). The GM is deeply influenced by several environmental factors, especially the diet, and recent findings highlighted significant symptom improvement in FM patients following various nutritional interventions such as vegetarian diet, low-fermentable oligosaccharides, disaccharides, monosaccharides, and polyols based diets, gluten-free diet, and especially an ancient grain supplementation. In particular, a recent study reported that a replacement diet with ancient Khorasan wheat led to an overall improvement in symptom severity of FM patients.
AIM OBJECTIVE
To examine the effects of ancient Khorasan wheat on the GM, inflammation, and short-chain fatty acid production in FM patients.
METHODS METHODS
After a 2-wk run-in period, 20 FM patients were enrolled in this randomized, double-blind crossover trial. In detail, they were assigned to consume either Khorasan or control wheat products for 8 wk and then, following an 8-wk washout period, crossed. Before and after treatments, GM characterization was performed by 16S rRNA sequencing while the fecal molecular inflammatory response and the short-chain fatty acids (SCFAs) were respectively determined with the Luminex MAGPIX detection system and a mass chromatography-mass spectrometry method.
RESULTS RESULTS
The Khorasan wheat replacement diet, in comparison with the control wheat diet, had more positive effects on intestinal microbiota composition and on both the fecal immune and SCFAs profiles such as the significant increase of butyric acid levels (
CONCLUSION CONCLUSIONS
The replacement diet based on ancient Khorasan wheat results in beneficial GM compositional and functional modifications that positively correlate with an improvement of FM symptomatology.

Identifiants

pubmed: 35664958
doi: 10.3748/wjg.v28.i18.1965
pmc: PMC9150053
doi:

Substances chimiques

Fatty Acids, Volatile 0
RNA, Ribosomal, 16S 0

Types de publication

Journal Article Randomized Controlled Trial

Langues

eng

Sous-ensembles de citation

IM

Pagination

1965-1980

Informations de copyright

©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.

Déclaration de conflit d'intérêts

Conflict-of-interest statement: The authors declare no competing interests for this article.

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Auteurs

Simone Baldi (S)

Department of Experimental and Clinical Medicine, University of Florence, Florence 50134, Italy.

Giuditta Pagliai (G)

Department of Experimental and Clinical Medicine, University of Florence, Florence 50134, Italy.

Monica Dinu (M)

Department of Experimental and Clinical Medicine, University of Florence, Florence 50134, Italy.

Leandro Di Gloria (L)

Department of Experimental and Clinical Medicine, University of Florence, Florence 50134, Italy.

Giulia Nannini (G)

Department of Experimental and Clinical Medicine, University of Florence, Florence 50134, Italy.

Lavinia Curini (L)

Department of Experimental and Clinical Medicine, University of Florence, Florence 50134, Italy.

Marco Pallecchi (M)

Department of Neurosciences, Psychology, Drug Research and Child Health, Section of Pharmaceutical and Nutraceutical Sciences, University of Florence, Sesto Fiorentino 50019, Italy.

Edda Russo (E)

Department of Experimental and Clinical Medicine, University of Florence, Florence 50134, Italy.

Elena Niccolai (E)

Department of Experimental and Clinical Medicine, University of Florence, Florence 50134, Italy.

Giovanna Danza (G)

Department of Biomedical, Experimental and Clinical Sciences "Mario Serio", University of Florence, Florence 50134, Italy.

Stefano Benedettelli (S)

Department of Agriculture, Food, Environment and Forestry, University of Florence, Florence 50144, Italy.

Giovanna Ballerini (G)

Multidisciplinary Center for Pain Therapy, Reference Center for Fibromyalgia, Piero Palagi Hospital, USL Toscana Centro, Florence 50122, Italy.

Barbara Colombini (B)

Department of Experimental and Clinical Medicine, University of Florence, Florence 50134, Italy.

Gianluca Bartolucci (G)

Department of Neurosciences, Psychology, Drug Research and Child Health, Section of Pharmaceutical and Nutraceutical Sciences, University of Florence, Sesto Fiorentino 50019, Italy.

Matteo Ramazzotti (M)

Department of Biomedical, Experimental and Clinical Sciences "Mario Serio", University of Florence, Florence 50134, Italy.

Francesco Sofi (F)

Department of Experimental and Clinical Medicine, University of Florence, Florence 50134, Italy.

Amedeo Amedei (A)

Department of Experimental and Clinical Medicine, University of Florence, Florence 50134, Italy.

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