Establishment of Wolbachia infection in Aedes aegypti from Pakistan via embryonic microinjection and semi-field evaluation of general fitness of resultant mosquito population.
Aedes aegypti
Aedes albopictus
Cytoplasmic incompatibility
Embryonic microinjection
Wolbachia
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
06 Jun 2022
06 Jun 2022
Historique:
received:
04
11
2021
accepted:
09
05
2022
entrez:
6
6
2022
pubmed:
7
6
2022
medline:
9
6
2022
Statut:
epublish
Résumé
Dengue is a mosquito-borne viral disease that is mainly spread by Aedes aegypti. It is prevalent on five continents, predominantly in tropical and sub-tropical zones across the world. Wolbachia bacteria have been extensively used in vector control strategies worldwide. The focus of the current study was to obtain a natural population of Ae. aegypti harbouring Wolbachia and to determine the impact of this bacteria on the new host in a semi-field environment. Wolbachia-infected Aedes albopictus was collected from the city of Lahore, Punjab, Pakistan, and Wolbachia were successfully introduced into laboratory-reared Ae. aegypti via embryonic microinjection. The stable vertical transmission of wAlbB in the host population was observed for eight generations, and the impact of Wolbachia on the general fitness of the host was evaluated in semi-field conditions. In the laboratory and semi-field experiments, wAlbB Wolbachia presented a strong cytoplasmic incompatibility (CI) effect, evidenced as zero egg hatching, in crosses between Wolbachia-infected males and wild (uninfected) females of Ae. aegypti. Wolbachia infection had no noticeable impact on the general fitness (P > 0.05), fecundity, body size (females and males) and mating competitiveness of the new host, Ae. aegypti. However, there was a significant decrease in female fertility (egg hatch) (P < 0.001). In addition, under starvation conditions, there was a remarkable decrease (P < 0.0001) in the life span of Wolbachia-infected females compared to uninfected females (4 vs. > 5 days, respectively). Wolbachia strain wAlbB has a great potential to control the dengue vector in Ae. aegypti populations by producing 100% CI with a limited burden on its host in natural field conditions. This strain can be used as a biological tool against vector-borne diseases.
Sections du résumé
BACKGROUND
BACKGROUND
Dengue is a mosquito-borne viral disease that is mainly spread by Aedes aegypti. It is prevalent on five continents, predominantly in tropical and sub-tropical zones across the world. Wolbachia bacteria have been extensively used in vector control strategies worldwide. The focus of the current study was to obtain a natural population of Ae. aegypti harbouring Wolbachia and to determine the impact of this bacteria on the new host in a semi-field environment.
METHODS
METHODS
Wolbachia-infected Aedes albopictus was collected from the city of Lahore, Punjab, Pakistan, and Wolbachia were successfully introduced into laboratory-reared Ae. aegypti via embryonic microinjection. The stable vertical transmission of wAlbB in the host population was observed for eight generations, and the impact of Wolbachia on the general fitness of the host was evaluated in semi-field conditions.
RESULTS
RESULTS
In the laboratory and semi-field experiments, wAlbB Wolbachia presented a strong cytoplasmic incompatibility (CI) effect, evidenced as zero egg hatching, in crosses between Wolbachia-infected males and wild (uninfected) females of Ae. aegypti. Wolbachia infection had no noticeable impact on the general fitness (P > 0.05), fecundity, body size (females and males) and mating competitiveness of the new host, Ae. aegypti. However, there was a significant decrease in female fertility (egg hatch) (P < 0.001). In addition, under starvation conditions, there was a remarkable decrease (P < 0.0001) in the life span of Wolbachia-infected females compared to uninfected females (4 vs. > 5 days, respectively).
CONCLUSIONS
CONCLUSIONS
Wolbachia strain wAlbB has a great potential to control the dengue vector in Ae. aegypti populations by producing 100% CI with a limited burden on its host in natural field conditions. This strain can be used as a biological tool against vector-borne diseases.
Identifiants
pubmed: 35668540
doi: 10.1186/s13071-022-05317-4
pii: 10.1186/s13071-022-05317-4
pmc: PMC9169386
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
191Informations de copyright
© 2022. The Author(s).
Références
FEMS Microbiol Lett. 2019 Dec 1;366(23):
pubmed: 31750894
FEMS Microbiol Lett. 2018 Apr 1;365(7):
pubmed: 29579215
mSystems. 2020 Jan 14;5(1):
pubmed: 31937677
Nature. 2011 Aug 24;476(7361):454-7
pubmed: 21866160
PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0009179
pubmed: 33591971
Appl Environ Microbiol. 2021 Sep 28;87(20):e0126421
pubmed: 34379518
Genes (Basel). 2020 Jul 25;11(8):
pubmed: 32722516
J Gen Virol. 2020 Feb;101(2):216-225
pubmed: 31846415
Proc Natl Acad Sci U S A. 2004 Oct 19;101(42):15042-5
pubmed: 15469918
PLoS Pathog. 2018 Jan 25;14(1):e1006815
pubmed: 29370307
Bull Entomol Res. 2015 Jun;105(3):305-15
pubmed: 25772521
Curr Biol. 2019 Dec 16;29(24):4241-4248.e5
pubmed: 31761702
Parasit Vectors. 2014 Jul 21;7:336
pubmed: 25041943
Front Microbiol. 2019 May 07;10:964
pubmed: 31134014
Appl Environ Microbiol. 2006 Nov;72(11):6934-7
pubmed: 16950898
Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12534-9
pubmed: 16895998
Acta Trop. 2016 Dec;164:473-481
pubmed: 27784636
Gates Open Res. 2020 May 11;4:50
pubmed: 32803130
Parasit Vectors. 2015 Jun 26;8:348
pubmed: 26112698
Parasit Vectors. 2018 May 11;11(1):295
pubmed: 29751814
Heredity (Edinb). 2004 Aug;93(2):135-42
pubmed: 15127087
Nature. 2019 Aug;572(7767):56-61
pubmed: 31316207
Proc Natl Acad Sci U S A. 2012 Jan 3;109(1):255-60
pubmed: 22123944
PLoS Pathog. 2020 Jul 29;16(7):e1008410
pubmed: 32726353
Sci Rep. 2017 Mar 16;7(1):210
pubmed: 28303006
Ecol Evol. 2020 Jan 16;10(3):1581-1591
pubmed: 32076535
Parasit Vectors. 2015 Oct 28;8:563
pubmed: 26510523
Nat Commun. 2016 May 31;7:11772
pubmed: 27243367
Science. 2005 Oct 14;310(5746):326-8
pubmed: 16224027
Trop Med Infect Dis. 2019 Jan 13;4(1):
pubmed: 30642130
PLoS Negl Trop Dis. 2012;6(7):e1754
pubmed: 22848774
PLoS Pathog. 2017 Jan 5;13(1):e1006006
pubmed: 28056065
PLoS Pathog. 2020 Apr 13;16(4):e1008433
pubmed: 32282862
J Med Entomol. 2002 Jul;39(4):699-704
pubmed: 12144308
J Virol. 2013 Jan;87(2):851-8
pubmed: 23115298
Environ Microbiol. 2004 Jan;6(1):35-44
pubmed: 14686939
Am J Trop Med Hyg. 2018 Jan;98(1):154-161
pubmed: 29141766
Cell. 2009 Dec 24;139(7):1268-78
pubmed: 20064373
Sci Rep. 2020 Jun 29;10(1):10551
pubmed: 32601334
PLoS Negl Trop Dis. 2013 Jun 06;7(6):e2250
pubmed: 23755311
Insects. 2020 Aug 13;11(8):
pubmed: 32823726
Science. 2009 Jan 2;323(5910):141-4
pubmed: 19119237
Front Microbiol. 2017 Mar 08;8:366
pubmed: 28337184
PLoS Pathog. 2010 Apr 01;6(4):e1000833
pubmed: 20368968
Parasit Vectors. 2013 Feb 11;6:36
pubmed: 23399027
Med Vet Entomol. 2009 Jun;23(2):132-40
pubmed: 19292821
PLoS One. 2016 Mar 18;11(3):e0151864
pubmed: 26990981
Proc Biol Sci. 2006 Jun 7;273(1592):1317-22
pubmed: 16777718
Evol Appl. 2015 Sep;8(8):751-68
pubmed: 26366194
Insect Biochem Mol Biol. 2005 Aug;35(8):903-10
pubmed: 15944085
J Theor Biol. 2012 Mar 21;297:26-32
pubmed: 22192469
PLoS Pathog. 2016 Feb 18;12(2):e1005434
pubmed: 26891349
Nat Microbiol. 2017 Mar 01;2:17007
pubmed: 28248294
PLoS Negl Trop Dis. 2021 Jul 12;15(7):e0009556
pubmed: 34252106
Am J Trop Med Hyg. 2016 Mar;94(3):507-16
pubmed: 26711515
Proc Natl Acad Sci U S A. 2021 Oct 12;118(41):
pubmed: 34607949
Trop Med Int Health. 2000 Apr;5(4):275-9
pubmed: 10810023
Parasit Vectors. 2020 Jan 14;13(1):28
pubmed: 31937373
Philos Trans R Soc Lond B Biol Sci. 2021 Feb 15;376(1818):20190809
pubmed: 33357050
PLoS Negl Trop Dis. 2020 Apr 3;14(4):e0008204
pubmed: 32243448
Annu Rev Genet. 2019 Dec 3;53:93-116
pubmed: 31505135
PLoS Negl Trop Dis. 2009 Sep 01;3(9):e508
pubmed: 19721700