A High-Throughput Yellow Fever Neutralization Assay.

Japanese encephalitis virus PRNT Zika virus dengue virus high-throughput neutralization assay reporter virus serodiagnosis yellow fever virus

Journal

Microbiology spectrum
ISSN: 2165-0497
Titre abrégé: Microbiol Spectr
Pays: United States
ID NLM: 101634614

Informations de publication

Date de publication:
29 06 2022
Historique:
pubmed: 8 6 2022
medline: 2 7 2022
entrez: 7 6 2022
Statut: ppublish

Résumé

Quick and accurate detection of neutralizing antibodies (nAbs) against yellow fever is essential in serodiagnosis during outbreaks for surveillance and to evaluate vaccine efficacy in population-wide studies. All of this requires serological assays that can process a large number of samples in a highly standardized format. Albeit being laborious, time-consuming, and limited in throughput, the classical plaque reduction neutralization test (PRNT) is still considered the gold standard for the detection and quantification of nAbs due to its sensitivity and specificity. Here, we report the development of an alternative fluorescence-based serological assay (SNT

Identifiants

pubmed: 35670599
doi: 10.1128/spectrum.02548-21
pmc: PMC9241659
doi:

Substances chimiques

Antibodies, Neutralizing 0
Antibodies, Viral 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0254821

Références

Wkly Epidemiol Rec. ;92(16):193-204
pubmed: 28429585
J Travel Med. 2015 Jan-Feb;22(1):48-56
pubmed: 25308927
Vaccine. 1996 Aug;14(11):1028-30
pubmed: 8879097
Antiviral Res. 2020 Oct;182:104884
pubmed: 32750466
Proc Natl Acad Sci U S A. 2013 May 14;110(20):E1867-76
pubmed: 23630248
Clin Vaccine Immunol. 2016 Aug 05;23(8):689-97
pubmed: 27307452
Appl Microbiol. 1968 Nov;16(11):1770-5
pubmed: 4973067
NPJ Vaccines. 2020 Jul 6;5(1):54
pubmed: 32655896
Emerg Microbes Infect. 2018 Jul 12;7(1):129
pubmed: 30002363
Vaccine. 1999 Mar 5;17(9-10):1042-6
pubmed: 10195613
Biochem Med (Zagreb). 2015 Jun 05;25(2):141-51
pubmed: 26110027
Nature. 2016 Apr 14;532(7598):155-6
pubmed: 27075072
Med Microbiol Immunol. 2002 Mar;190(4):199-202
pubmed: 12005333
J Exp Med. 1937 May 31;65(6):787-800
pubmed: 19870634
Vaccine. 2014 Oct 7;32(44):5769-75
pubmed: 25192973
Nat Methods. 2005 Dec;2(12):905-9
pubmed: 16299475
J Med Virol. 1986 Aug;19(4):307-11
pubmed: 2943870
NPJ Vaccines. 2018 Dec 13;3:56
pubmed: 30564463
Lancet. 2018 Dec 1;392(10162):2347
pubmed: 30527606
Vaccine. 2017 Oct 20;35(44):5951-5955
pubmed: 28366605
J Infect Dis. 2018 Jul 13;218(4):606-613
pubmed: 29659924
Med Microbiol Immunol. 2017 Apr;206(2):175-185
pubmed: 28176006
J Virol Methods. 2015 Dec 1;225:41-8
pubmed: 26342907
Pediatr Infect Dis J. 2016 Oct;35(10):1140-7
pubmed: 27254034
mBio. 2020 Apr 7;11(2):
pubmed: 32265332
Lancet Glob Health. 2016 Aug;4(8):e534-47
pubmed: 27364568
Vaccine. 2011 Aug 26;29(37):6327-34
pubmed: 21640779
Int J Infect Dis. 2021 Jul;108:465-472
pubmed: 34082090
Lancet. 2016 Apr 16;387(10028):1599-600
pubmed: 27116054
Lancet. 2008 Feb 9;371(9611):500-9
pubmed: 18262042
Infect Ecol Epidemiol. 2016 Apr 15;6:31099
pubmed: 27087689
J Gen Virol. 2017 Nov;98(11):2712-2724
pubmed: 29022864
Science. 2016 Apr 8;352(6282):128-9
pubmed: 27124428
J Travel Med. 1999 Dec;6(4):228-33
pubmed: 10575170
J Travel Med. 2012 Mar-Apr;19(2):122-3
pubmed: 22414038
Antiviral Res. 2020 Nov;183:104929
pubmed: 32898584
J Infect Dis. 2020 Jun 11;221(12):1922-1924
pubmed: 31545366
Pathol Res Pract. 1992 Feb;188(1-2):177-81
pubmed: 1594489
Biologicals. 2012 Nov;40(6):399-404
pubmed: 23034357

Auteurs

Madina Rasulova (M)

KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Virology and Chemotherapy, Molecular Vaccinology & Vaccine Discovery, Leuven, Belgium.
KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Translational Platform Virology and Chemotherapy (TPVC), Leuven, Belgium.

Thomas Vercruysse (T)

KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Virology and Chemotherapy, Molecular Vaccinology & Vaccine Discovery, Leuven, Belgium.
KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Translational Platform Virology and Chemotherapy (TPVC), Leuven, Belgium.

Jasmine Paulissen (J)

KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Virology and Chemotherapy, Molecular Vaccinology & Vaccine Discovery, Leuven, Belgium.
KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Translational Platform Virology and Chemotherapy (TPVC), Leuven, Belgium.

Catherina Coun (C)

KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Virology and Chemotherapy, Molecular Vaccinology & Vaccine Discovery, Leuven, Belgium.
KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Translational Platform Virology and Chemotherapy (TPVC), Leuven, Belgium.

Vanessa Suin (V)

Sciensano, Viral Diseases Service, Scientific Directorate of Infectious Diseases in Humans, Brussels, Belgium.

Leo Heyndrickx (L)

Virology Unit, Department of Biomedical Sciences, Institute of Tropical Medicine Antwerpgrid.11505.30, Antwerp, Belgium.

Ji Ma (J)

KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Virology and Chemotherapy, Molecular Vaccinology & Vaccine Discovery, Leuven, Belgium.
Global Virus Network (GVN), Baltimore, Maryland, USA.

Katrien Geerts (K)

KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Virology and Chemotherapy, Molecular Vaccinology & Vaccine Discovery, Leuven, Belgium.
Global Virus Network (GVN), Baltimore, Maryland, USA.

Jolien Timmermans (J)

KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Virology and Chemotherapy, Molecular Vaccinology & Vaccine Discovery, Leuven, Belgium.
KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Translational Platform Virology and Chemotherapy (TPVC), Leuven, Belgium.

Niraj Mishra (N)

KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Virology and Chemotherapy, Molecular Vaccinology & Vaccine Discovery, Leuven, Belgium.
Global Virus Network (GVN), Baltimore, Maryland, USA.

Li-Hsin Li (LH)

KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Virology and Chemotherapy, Molecular Vaccinology & Vaccine Discovery, Leuven, Belgium.
Global Virus Network (GVN), Baltimore, Maryland, USA.

Dieudonné Buh Kum (DB)

KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Virology and Chemotherapy, Molecular Vaccinology & Vaccine Discovery, Leuven, Belgium.
Global Virus Network (GVN), Baltimore, Maryland, USA.

Lotte Coelmont (L)

KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Virology and Chemotherapy, Molecular Vaccinology & Vaccine Discovery, Leuven, Belgium.
Global Virus Network (GVN), Baltimore, Maryland, USA.

Steven Van Gucht (S)

Sciensano, Viral Diseases Service, Scientific Directorate of Infectious Diseases in Humans, Brussels, Belgium.

Hadi Karimzadeh (H)

Division of Clinical Pharmacology, University Hospital, LMU Munich, Munich, Germany.
Unit Clinical Pharmacology (EKliP), Helmholtz Center for Environmental Health, Munich, Germany.

Julia Thorn-Seshold (J)

Division of Clinical Pharmacology, University Hospital, LMU Munich, Munich, Germany.
Unit Clinical Pharmacology (EKliP), Helmholtz Center for Environmental Health, Munich, Germany.

Simon Rothenfußer (S)

Division of Clinical Pharmacology, University Hospital, LMU Munich, Munich, Germany.
Unit Clinical Pharmacology (EKliP), Helmholtz Center for Environmental Health, Munich, Germany.

Kevin K Ariën (KK)

Virology Unit, Department of Biomedical Sciences, Institute of Tropical Medicine Antwerpgrid.11505.30, Antwerp, Belgium.
Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium.

Johan Neyts (J)

KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Virology and Chemotherapy, Molecular Vaccinology & Vaccine Discovery, Leuven, Belgium.
Global Virus Network (GVN), Baltimore, Maryland, USA.

Kai Dallmeier (K)

KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Virology and Chemotherapy, Molecular Vaccinology & Vaccine Discovery, Leuven, Belgium.
Global Virus Network (GVN), Baltimore, Maryland, USA.

Hendrik Jan Thibaut (HJ)

KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Virology and Chemotherapy, Molecular Vaccinology & Vaccine Discovery, Leuven, Belgium.
KU Leuvengrid.5596.f Department of Microbiology, Immunology and Transplantation, Rega Institute, Translational Platform Virology and Chemotherapy (TPVC), Leuven, Belgium.

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