Chlorella vulgaris ameliorates testicular toxicity induced by carbon tetrachloride in male rats via modulating oxidative stress.
DNA fragmentation
carbon tetrachloride
chlorella vulgaris
oxidative stress
sperm
Journal
Andrologia
ISSN: 1439-0272
Titre abrégé: Andrologia
Pays: Germany
ID NLM: 0423506
Informations de publication
Date de publication:
Oct 2022
Oct 2022
Historique:
revised:
15
05
2022
received:
04
02
2022
accepted:
18
05
2022
pubmed:
8
6
2022
medline:
8
9
2022
entrez:
7
6
2022
Statut:
ppublish
Résumé
This study was aimed to evaluate the protective effects of Chlorella Vulgaris (CVE) (50 and 100 mg/kg doses) on sperm DNA fragmentation, testis oxidative stress in Carbon tetrachloride (CCL4)-exposed rats. Thirty healthy male Wistar rats were divided into five groups (n = 6): Control; CCl4; CVE; CCl4 + CVE50; CCl4 + CVE100. At the end of the experiment, the testicular oxidative stress parameters were estimated. The Chromomycin A3 (CMA3) and Acridine orange (AO) staining were performed to examine the sperm DNA fragmentation status. CCl4 treatment showed a significant decrease in antioxidant markers and sperm count, viability, normal morphology and motility as well as significantly increased the testicular oxidative stress markers, and the percentage of CMA3 and AO positive sperms in normal rats (p < 0.05). While CVE supplementation has revealed a significant decrease in the percentage of CMA3 and AO positive sperms as well as testicular oxidative stress markers and considerably improved the testis antioxidant status (p < 0.05). CVE has also increased the number of sperms with forwarding movement, normal morphology and viability (p < 0.05). Taken together, our analyses suggest that CVE may play a critical role in attenuating the CCl4-induced oxidative stress in the testis, thereby protecting the sperm membrane and DNA against oxidative damage.
Substances chimiques
Antioxidants
0
Carbon Tetrachloride
CL2T97X0V0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14495Subventions
Organisme : Hamadan University of Medical Sciences
Informations de copyright
© 2022 Wiley-VCH GmbH.
Références
Abd El Latif, A., Assar, D. H., Elkaw, E. M., Hamza, H. A., Alkhalifah, D. H. M., Hozzein, W. N., & Hamouda, R. A. (2021). Protective role of Chlorella vulgaris with thiamine against paracetamol induced toxic effects on haematological, biochemical, oxidative stress parameters and histopathological changes in Wistar rats. Scientific Reports, 11(1), 1-16.
Abd El-Hakim, Y. M., Abdel-Rahman Mohamed, A., Khater, S. I., Hamed Arisha, A., Metwally, M. M. M., Nassan, M. A., & Hassan, M. E. (2021). Chitosan-stabilized selenium nanoparticles and metformin synergistically rescue testicular oxidative damage and steroidogenesis-related genes dysregulation in high-fat diet/Streptozotocin-induced diabetic rats. Antioxidants, 10(1), 17.
Adewoyin, M., Ibrahim, M., Roszaman, R., Isa, M. L. M., Alewi, N. A. M., Rafa, A. A. A., & Anuar, M. N. N. (2017). Male infertility: The effect of natural antioxidants and phytocompounds on seminal oxidative stress. Diseases, 5(1), 9.
Agarwal, A., Virk, G., Ong, C., & Du Plessis, S. S. (2014). Effect of oxidative stress on male reproduction. The World Journal of Men's Health, 32(1), 1-17.
Aitken, R. J., & Roman, S. D. (2008). Antioxidant systems and oxidative stress in the testes. Oxidative Medicine and Cellular Longevity, 1(1), 15-24. https://doi.org/10.4161/oxim.1.1.6843
Ali, M., Martinez, M., & Parekh, N. (2021). Are antioxidants a viable treatment option for male infertility? Andrologia, 53(1), e13644.
Barati, E., Nikzad, H., & Karimian, M. (2020). Oxidative stress and male infertility: Current knowledge of pathophysiology and role of antioxidant therapy in disease management. Cellular and Molecular Life Sciences, 77(1), 93-113.
Birben, E., Sahiner, U. M., Sackesen, C., Erzurum, S., & Kalayci, O. (2012). Oxidative stress and antioxidant defense. World Allergy Organization Journal, 5(1), 9-19. https://doi.org/10.1097/WOX.0b013e3182439613
Cocuzza, M., Sikka, S. C., Athayde, K. S., & Agarwal, A. (2007). Clinical relevance of oxidative stress and sperm chromatin damage in male infertility: An evidence based analysis. International Brazilian Journal of Urology, 33(5), 603-621.
Conrad, M., Moreno, S., Sinowatz, F., Ursini, F., Kölle, S., Roveri, A., Brielmeier, M., Wurst, W., Maiorino, M., & Bornkamm, G. (2005). The nuclear form of phospholipid hydroperoxide glutathione peroxidase is a protein thiol peroxidase contributing to sperm chromatin stability. Molecular and Cellular Biology, 25(17), 7637-7644.
Dorostghoal, M., Kazeminejad, S., Shahbazian, N., Pourmehdi, M., & Jabbari, A. (2017). Oxidative stress status and sperm DNA fragmentation in fertile and infertile men. Andrologia, 49(10), e12762.
Eissa, M. M., Ahmed, M. M., Abd Eldaim, M. A., Orabi, S. H., Elbaz, H. T., Mohamed, M. A., Elweza, A. E., & Mousa, A. A. (2020). Methanolic extract of Chlorella vulgaris protects against sodium nitrite-induced reproductive toxicity in male rats. Andrologia, 52(11), e13811.
Elewa, Y. H., Mohamed, A. A.-R., Galal, A. A., El-Naseery, N. I., Ichii, O., & Kon, Y. (2019). Food Yellow4 reprotoxicity in relation to localization of DMC1 and apoptosis in rat testes: Roles of royal jelly and cod liver oil. Ecotoxicology and Environmental Safety, 169, 696-706.
Guenaou, I., Hmimid, F., Lahlou, F. A., Errami, A., Irahal, I. N., Fahde, S., Ouafik, L., & Bourhim, N. (2021). Cytoprotective effect of ethyl acetate fraction from Ephedra fragilis on H2O2-induced oxidative damage in Tetrahymena pyriformis. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 239, 108899.
Hefnawy, H. T. M., & Ramadan, M. F. (2013). Protective effects of Lactuca sativa ethanolic extract on carbon tetrachloride induced oxidative damage in rats. Asian Pacific Journal of Tropical Disease, 3(4), 277-285.
Inoue, H., & Nishida, E. (2010). The DM domain transcription factor MAB-3 regulates male hypersensitivity to oxidative stress in Caenorhabditis elegans. Molecular and Cellular Biology, 30(14), 3453-3459.
Iranpour, F. G., Nasr-Esfahani, M. H., Valojerdi, M. R., & Taki Al-Taraihi, T. M. (2000). Chromomycin A3 staining as a useful tool for evaluation of male fertility. Journal of Assisted Reproduction and Genetics, 17(1), 60-66.
Khan, R. A. (2012). Protective effects of Launaea procumbens on rat testis damage by CCl4. Lipids in Health and Disease, 11(1), 1-8.
Ko, E. Y., Sabanegh, E. S., Jr., & Agarwal, A. (2014). Male infertility testing: Reactive oxygen species and antioxidant capacity. Fertility and Sterility, 102(6), 1518-1527.
Kullisaar, T., Türk, S., Kilk, K., Ausmees, K., Punab, M., & Mändar, R. (2013). Increased levels of hydrogen peroxide and nitric oxide in male partners of infertile couples. Andrology, 1(6), 850-858.
Kumar, N., & Singh, A. K. (2018). Reactive oxygen species in seminal plasma as a cause of male infertility. Journal of Gynecology Obstetrics and Human Reproduction, 47(10), 565-572.
Mandal, R., Badyakar, D., & Chakrabarty, J. (2014). Role of membrane lipid fatty acids in sperm cryopreservation. Advances in Andrology, 2014, 1-9.
Mazani, M., Ojarudi, M., Banaei, S., Salimnejad, R., Latifi, M., Azizi, H., & Rezagholizadeh, L. (2020). The protective effect of cinnamon and ginger hydro-alcoholic extract on carbon tetrachloride-induced testicular damage in rats. Andrologia, 52(7), e13651.
Mohamed, A. A., Abdellatief, S. A., Khater, S. I., Ali, H., & Al-Gabri, N. A. (2019). Fenpropathrin induces testicular damage, apoptosis, and genomic DNA damage in adult rats: Protective role of camel milk. Ecotoxicology and Environmental Safety, 181, 548-558. https://doi.org/10.1016/j.ecoenv.2019.06.047
Mohseni, R., Arab Sadeghabadi, Z., Karimi, J., Gholami, H., Ghasemi, H., Ghadimipour, H. R., & Kheiripour, N. (2021). Chlorella vulgaris supplementation attenuates the progression of liver fibrosis through targeting TGF-β-signaling pathway in the CCl4-induced liver fibrosis in rats. Toxin Reviews, 40(4), 1347-1355.
Moradi, M. N., Behrouj, H., Alipoor, B., Kheiripour, N., Ghasemi, H., & Ghasemi, H. (2021). Chlorella vulgaris is an effective supplement in counteracting non-alcoholic fatty liver disease-related complications through modulation of dyslipidemia, insulin resistance, and inflammatory pathways. Journal of Food Biochemistry, 45(10), e13914.
Moridi, H., Hosseini, S. A., Shateri, H., Kheiripour, N., Kaki, A., Hatami, M., & Ranjbar, A. (2018). Protective effect of cerium oxide nanoparticle on sperm quality and oxidative damage in malathion-induced testicular toxicity in rats: An experimental study. International Journal of Reproductive BioMedicine, 16(4), 261-266.
Mtaki, K., Kyewalyanga, M. S., & Mtolera, M. S. (2020). Assessment of antioxidant contents and free radical-scavenging capacity of Chlorella vulgaris cultivated in low cost media. Applied Sciences, 10(23), 8611.
Mustafa, H. N. (2015). Potential alleviation of Chlorella vulgaris and Zingiber officinale on lead-induced testicular toxicity: An ultrastructural study. Folia Biologica (Kraków), 63(4), 269-278.
Nasr-Esfahani, M. H., Razavi, S., & Mardani, M. (2001). Andrology: Relation between different human sperm nuclear maturity tests and in vitro fertilization. Journal of Assisted Reproduction and Genetics, 18(4), 221-227.
Omran, G. A., Gaber, H. D., Mostafa, N. A. M., Abdel-Gaber, R. M., & Salah, E. A. (2018). Potential hazards of bisphenol a exposure to semen quality and sperm DNA integrity among infertile men. Reproductive Toxicology, 81, 188-195.
Osama, E., Galal, A. A. A., Abdalla, H., & El-Sheikh, S. M. A. (2019). Chlorella vulgaris ameliorates testicular toxicity induced by deltamethrin in male rats via modulating oxidative stress. Andrologia, 51(3), e13214. https://doi.org/10.1111/and.13214
Panahi, Y., Darvishi, B., Jowzi, N., Beiraghdar, F., & Sahebkar, A. (2016). Chlorella vulgaris: A multifunctional dietary supplement with diverse medicinal properties. Current Pharmaceutical Design, 22(2), 164-173.
Pasqualotto, F. F., Sharma, R. K., Nelson, D. R., Thomas, A. J., Jr., & Agarwal, A. (2000). Relationship between oxidative stress, semen characteristics, and clinical diagnosis in men undergoing infertility investigation. Fertility and Sterility, 73(3), 459-464.
Peña, S. T., Jr., Gummow, B., Parker, A. J., & Paris, D. B. (2019). Antioxidant supplementation mitigates DNA damage in boar (Sus scrofa domesticus) spermatozoa induced by tropical summer. PLoS One, 14(4), e0216143.
Safi, C., Zebib, B., Merah, O., Pontalier, P.-Y., & Vaca-Garcia, C. (2014). Morphology, composition, production, processing and applications of Chlorella vulgaris: A review. Renewable and Sustainable Energy Reviews, 35, 265-278.
Salehi, P., Shahrokhi, S. Z., Kamran, T., Ajami, A., Taghiyar, S., & Deemeh, M. R. (2019). Effect of antioxidant therapy on the sperm DNA integrity improvement; a longitudinal cohort study. International Journal of Reproductive BioMedicine, 17(2), 99.
Shateri, H., Ranjbar, A., Kheiripour, N., Ghasemi, H., Pourfarjam, Y., Habibitabar, E., Gholami, H., & Moridi, H. (2019). Tempol improves oxidant/antioxidant parameters in testicular tissues of diabetic rats. Life Sciences, 221, 65-71.
Shokri, S., Hemadi, M., Bayat, G., Bahmanzadeh, M., Jafari-Anarkooli, I., & Mashkani, Β. (2014). Combination of running exercise and high dose of anabolic androgenic steroid, nandrolone decanoate, increases protamine deficiency and DNA damage in rat spermatozoa. Andrologia, 46(2), 184-190.
Sikiru, A. B., Arangasamy, A., Alemede, I., Guvvala, P., Egena, S., Ippala, J., & Bhatta, R. (2019). Chlorella vulgaris supplementation effects on performances, oxidative stress and antioxidant genes expression in liver and ovaries of New Zealand white rabbits. Heliyon, 5(9), e02470.
Sikiru, A. B., Arunachalam, A., Egena, S. S. A., Veerasamy, S., Reddy, I. J., & Bhatta, R. (2020). Chlorella vulgaris biomass supplementation increased the abundance of hepatic antioxidant proteins in relation to oxidative stress reduction in rabbits. Research Square. https://doi.org/10.21203/rs.3.rs-67743/v1
Sönmez, M., Türk, G., Çeribaşı, S., Çiftçi, M., Yüce, A., Güvenç, M., Aksakal, M. (2014). Quercetin attenuates carbon tetrachloride-induced testicular damage in rats. Andrologia, 46(8), 848-858.
Vatannejad, A., Tavilani, H., Sadeghi, M. R., Amanpour, S., Shapourizadeh, S., & Doosti, M. (2017). Evaluation of ROS-TAC score and DNA damage in fertile normozoospermic and infertile asthenozoospermic males. Urology Journal, 14(1), 2973-2978.
Victor, I. E., Ugorji, U. O., & Adeyinka, A. (2014). Efficacy of Hibiscus sabdariffa and Telfairia occidentalis in the attenuation of CCl4-mediated oxidative stress. Asian Pacific Journal of Tropical Medicine, 7, S321-S326.
Vijayavel, K., Anbuselvam, C., & Balasubramanian, M. (2007). Antioxidant effect of the marine algae Chlorella vulgaris against naphthalene-induced oxidative stress in the albino rats. Molecular and Cellular Biochemistry, 303(1-2), 39-44.
Wei, D., Zhang, X. L., Wang, Y. Z., Yang, C. X., & Chen, G. (2010). Lipid peroxidation levels, total oxidant status and superoxide dismutase in serum, saliva and gingival crevicular fluid in chronic periodontitis patients before and after periodontal therapy. Australian Dental Journal, 55(1), 70-78.
Wright, C., Milne, S., & Leeson, H. (2014). Sperm DNA damage caused by oxidative stress: Modifiable clinical, lifestyle and nutritional factors in male infertility. Reproductive Biomedicine Online, 28(6), 684-703. https://doi.org/10.1016/j.rbmo.2014.02.004
Xie, D. E., Lu, C., Zhu, Y., Zhu, S., Yang, E.-J., & Jin, X. (2018). Analysis on the association between sperm DNA fragmentation index and conventional semen parameters, blood microelements and seminal plasma ROS in male patients with infertility. Experimental and Therapeutic Medicine, 15(6), 5173-5176.
Yu, M., Chen, M., Gui, J., Huang, S., Liu, Y., Shentu, H., He, J., Fang, Z., Wang, W., & Zhang, Y. (2019). Preparation of Chlorella vulgaris polysaccharides and their antioxidant activity in vitro and in vivo. International Journal of Biological Macromolecules, 137, 139-150.
Yüce, A., Türk, G., Çeribaşı, S., Güvenç, M., Çiftçi, M., Sönmez, M., Özer Kaya, Ş., Çay, M., & Aksakal, M. (2014). Effectiveness of cinnamon (Cinnamomum zeylanicum) bark oil in the prevention of carbon tetrachloride-induced damages on the male reproductive system. Andrologia, 46(3), 263-272.