Determining the Class and Subclass of Monoclonal Antibodies Using Antibody Capture on Antigen-Coated Plates.
Journal
Cold Spring Harbor protocols
ISSN: 1559-6095
Titre abrégé: Cold Spring Harb Protoc
Pays: United States
ID NLM: 101524530
Informations de publication
Date de publication:
07 06 2022
07 06 2022
Historique:
entrez:
7
6
2022
pubmed:
8
6
2022
medline:
10
6
2022
Statut:
epublish
Résumé
The class or subclass of an antibody is defined by its heavy chain. There are five main classes of antibodies: M, G, A, E, and D. By definition, a monoclonal antibody should only be of a single class or subclass. Each class of antibody is associated with specific functions. The method of antibody purification will differ based on the class. In this protocol, antigen is used to capture antibodies reactive to it. Specific class and/or subclass secondary antibodies are then used to discern which class/subclass has been captured.
Identifiants
pubmed: 35672131
pii: 2022/5/pdb.prot103226
doi: 10.1101/pdb.prot103226
doi:
Substances chimiques
Antibodies, Monoclonal
0
Immunoglobulin G
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
Pdb.prot103226Informations de copyright
© 2022 Cold Spring Harbor Laboratory Press.