Proximal sesamoid bone microdamage is localized to articular subchondral regions in Thoroughbred racehorses, with similar fracture toughness between fracture and controls.
Journal
Veterinary surgery : VS
ISSN: 1532-950X
Titre abrégé: Vet Surg
Pays: United States
ID NLM: 8113214
Informations de publication
Date de publication:
Aug 2022
Aug 2022
Historique:
revised:
04
01
2022
received:
08
06
2021
accepted:
24
02
2022
pubmed:
9
6
2022
medline:
2
8
2022
entrez:
8
6
2022
Statut:
ppublish
Résumé
To determine whether proximal sesamoid bone (PSB) microdamage and fracture toughness differ between Thoroughbred racehorses sustaining PSB fracture and controls. Cadaveric case-control. Twenty-four Thoroughbred racehorses (n = 12 PSB fracture, n = 12 control). Proximal sesamoid bones were dissected, and gross pathological changes and morphological measurements were documented. High-speed exercise history data were evaluated. Microdamage was assessed in fracture, fracture-contralateral limb (FXCL) and control PSBs using whole bone lead uranyl acetate (LUA) staining with micro-CT imaging or basic fuchsin histological analysis. Fracture toughness mechanical testing was carried out in 3-point-bending of microbeams created from PSB flexor cortices. Data were analyzed using ordinal logistic and linear regression models. Microdamage was detected most commonly in the articular subchondral region of PSBs via LUA micro-CT and basic fuchsin histology. There were no differences in microdamage between FXCL and control PSBs. Fracture toughness values were similar for FXCL (1.31 MPa√m) and control (1.35 MPa√m) PSBs. Exercise histories were similar except that horses sustaining fracture spent a greater percentage of their careers in rest weeks. Microdamage was detected in the articular region of PSBs but was not greater in horses sustaining catastrophic PSB fracture. Fracture toughness of PSB flexor cortices did not differ between FXCL and control PSBs. Although uncommon, microdamage is localized to the articular region of Thoroughbred racehorse PSBs. Catastrophic PSB failure is not associated with lower PSB flexor cortex fracture toughness.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
952-962Subventions
Organisme : Harry M. Zweig Memorial Fund for Equine Research to HLR
Organisme : Harry M. Zweig Resident Research Grant to LKL and HLR
Organisme : National Science Foundation:
ID : NSF CMMI 1452852 to ED and NSF DGE-1144153 to PMP
Organisme : National Institutes of Health: S10OD025049 to Cornell University Biotechnology Resource Center, National Institutes of Health, National Institutes of Health (S10OD025049 to Cornell University Biotechnology Resource Center)
Informations de copyright
© 2022 American College of Veterinary Surgeons.
Références
Johnson BJ, Stover SM, Daft BM, et al. Causes of death in racehorses over a 2 year period. Equine Vet J. 1994;26(4):327-330.
Anthenill LA, Stover SM, Gardner IA, et al. Association between findings on palmarodorsal radiographic images and detection of a fracture in the proximal sesamoid bones of forelimbs obtained from cadavers of racing thoroughbreds. Am J Vet Res. 2006;67(5):858-868.
Peloso JG, Vogler JB, Cohen ND, Marquis P & Hilt L Use of standing MRI and a new MRI grading system of the subchondral bone of the distal third metacarpal bone to identify bone changes in the thoroughbred racehorse when comparing 21 cases of catastrophic biaxial proximal sesamoid bone fracture with 53 controls. Equine Vet J. 2014;46: 24-24.
Anthenill LA, Gardner IA, Pool RR, Garcia TC, Stover SM. Comparison of macrostructural and microstructural bone features in thoroughbred racehorses with and without midbody fracture of the proximal sesamoid bone. Am J Vet Res. 2010;71:755-765.
Palmer SE, McDonough SP, Mohammed HO. Reduction of thoroughbred racing fatalities at New York racing association racetracks using a multi-disciplinary mortality review process. J Vet Diagn Invest. 2017;29(4):465-475.
Riggs CM. Fractures - a preventable hazard of racing thoroughbreds? Vet J. 2002;163(1):19-29.
Kristoffersen M, Hetzel U, Parkin TDH, Singer ER. Are bi-axial proximal sesamoid bone fractures in the british thoroughbred racehorse a bone fatigue related fracture?: a histological study. Vet Comp Orthop Traumatol. 2010;23(5):336-342.
Stewart HL, Kawcak CE. The importance of subchondral bone in the pathophysiology of osteoarthritis. Front Vet Sci. 2018;5(AUG:178.
Estberg L, Stover SM, Gardner IA, et al. Fatal musculoskeletal injuries incurred during racing and training in thoroughbreds. J Am Vet Med Assoc. 1996;208(1):92-96.
Whitton RC, Ayodele BA, Hitchens PL, Mackie EJ. Subchondral bone microdamage accumulation in distal metacarpus of thoroughbred racehorses. Equine Vet J. 2018;50(6):766-773.
Shaffer SK, To C, Garcia TC, Fyhrie DP, Uzal FA, Stover SM. Subchondral focal osteopenia associated with proximal sesamoid bone fracture in thoroughbred racehorses. Equine Vet J. 2021;53:294-305.
Spriet M, Espinosa-Mur P, Cissell DD, et al. 18F-sodium fluoride positron emission tomography of the racing thoroughbred fetlock: validation and comparison with other imaging modalities in nine horses. Equine Vet J. 2019;51(3):375-383.
Norrdin RW, Bay BK, Drews MJ, Martin RB, Stover SM. Overload arthrosis: strain patterns in the equine metacarpal condyle. J Musculoskelet Neuronal Interact. 2001;1(4):357-362.
Burr DB, Hooser M. Alterations to the en bloc basic fuchsin staining protocol for the demonstration of microdamage produced in vivo. Bone. 1995;17(4):431-433.
Burr DB, Stafford T. Validity of the bulk-staining technique to separate artifactual from in vivo bone microdamage. Clin Orthop Relat Res. 1990;260:305-308.
Poundarik AA, Vashishth D. Multiscale imaging of bone microdamage. Connect Tissue Res. 2015;56(2):87-98. doi:10.3109/03008207.2015.1008133.Multiscale
Tang SY, Vashishth D. A non-invasive in vitro technique for the three-dimensional quantification of microdamage in trabecular bone. Bone. 2007;40(5):1259-1264.
Cresswell EN, McDonough SP, Palmer SE, Hernandez CJ, Reesink HL. Can quantitative computed tomography detect bone morphological changes associated with catastrophic proximal sesamoid bone fracture in thoroughbred racehorses? Equine Vet J. 2019;51(1):123-130.
Peloso JG, Vogler JB, Cohen ND, Marquis P, Hilt L. Association of catastrophic biaxial fracture of the proximal sesamoid bones with bony changes of the metacarpophalangeal joint identified by standing magnetic resonance imaging in cadaveric forelimbs of thoroughbred racehorses. J Am Vet Med Assoc. 2015;246(6):661-673.
Shi L, Wang D, Riggs CM, Qin L, Griffith JF. Statistical analysis of bone mineral density using voxel-based morphometry-an application on proximal sesamoid bones in racehorses. J Orthop Res. 2011;29(8):1230-1236.
Ritchie RO, Koester KJ, Ionova S, Yao W, Lane NE, Ager JW. Measurement of the toughness of bone: a tutorial with special reference to small animal studies. Bone. 2008;43(5):798-812.
Taylor D. Measuring fracture toughness in biological materials. J Mech Behav Biomed Mater. 2018;77:776-782.
Bonfield W. Advances in the fracture mechanics of cortical bone. J Biomech. 1987;20(11-12):1071-1081.
Alto A, Pope MH. On the fracture toughness of equine metacarpi. J Biomech. 1979;12(6):415-421.
Cresswell EN, Ruspi BD, Wollman CW, et al. Determination of correlation of proximal sesamoid bone osteoarthritis with high-speed furlong exercise and catastrophic sesamoid bone fracture in thoroughbred racehorses. Am J Vet Res. 2021;82(6):467-477.
Diab T, Vashishth D. Effects of damage morphology on cortical bone fragility. Bone. 2005;37(1):96-102.
Ayodele BA, Hitchens PL, Wong ASM, Mackie EJ, Whitton RC. Microstructural properties of the proximal sesamoid bones of thoroughbred racehorses in training. Equine Vet J. 2021;53(6):1169-1177.
Ebacher V, Wang R. Circumferential arc-shaped microcracks in Haversian bone: Lead-uranyl acetate staining for micro-CT imaging. Materials Research Society Symposium Proceedings. Vol 1187; 2009:7-12.
Hart NH, Nimphius S, Rantalainen T, Ireland A, Siafarikas A, Newton RU. Mechanical basis of bone strength: influence of bone material, bone structure and muscle action. J Musculoskelet Neuronal Interact. 2017;17(3):114-139.
Thompson KN, Cheung TK. A finite element model of the proximal sesamoid bones of the horse under different loading conditions. Vet Comp Orthop Traumatol. 1994;07(01):35-39.
Cook RB, Zioupos P. The fracture toughness of cancellous bone. J Biomech. 2009;42(13):2054-2060.
Rubin CT, Seeherman H, Qin YX, Gross TS. The mechanical consequences of load bearing in the equine third metacarpal across speed and gait: the nonuniform distributions of normal strain, shear strain, and strain energy density. FASEB J. 2013;27:1887-1894.
Parkin TDH, Clegg PD, French NP, et al. Horse-level risk factors for fatal distal limb fracture in racing thoroughbreds in the UK. Equine Vet J. 2004;36(6):513-519.
Anthenill LA, Stover SM, Gardner IA, Hill AE. Risk factors for proximal sesamoid bone fractures associated with exercise history and horseshoe characteristics in thoroughbred racehorses. Am J Vet Res. 2007;68(7):760-771.
Holmes JM, Mirams M, Mackie EJ, Whitton RC. Thoroughbred horses in race training have lower levels of subchondral bone remodeling in highly loaded regions of the distal metacarpus compared to horses resting from training. Vet J. 2014;202(3):443-447.
Boyde A, Firth EC. Musculoskeletal responses of 2-year-old thoroughbred horses to early training. 8. Quantitative back-scattered electron scanning electron microscopy and confocal fluorescence microscopy of the epiphysis of the third metacarpal bone. N Z Vet J. 2005;53(2):123-132.