Imprinted antibody responses against SARS-CoV-2 Omicron sublineages.


Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
22 Aug 2022
Historique:
pubmed: 10 6 2022
medline: 10 6 2022
entrez: 9 6 2022
Statut: epublish

Résumé

SARS-CoV-2 Omicron sublineages carry distinct spike mutations and represent an antigenic shift resulting in escape from antibodies induced by previous infection or vaccination. We show that hybrid immunity or vaccine boosters result in potent plasma neutralizing activity against Omicron BA.1 and BA.2 and that breakthrough infections, but not vaccination-only, induce neutralizing activity in the nasal mucosa. Consistent with immunological imprinting, most antibodies derived from memory B cells or plasma cells of Omicron breakthrough cases cross-react with the Wuhan-Hu-1, BA.1 and BA.2 receptor-binding domains whereas Omicron primary infections elicit B cells of narrow specificity. While most clinical antibodies have reduced neutralization of Omicron, we identified an ultrapotent pan-variant antibody, that is unaffected by any Omicron lineage spike mutations and is a strong candidate for clinical development.

Identifiants

pubmed: 35677069
doi: 10.1101/2022.05.08.491108
pmc: PMC9176643
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NIAID NIH HHS
ID : DP1 AI158186
Pays : United States
Organisme : NIAID NIH HHS
ID : HHSN272201700059C
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI163019
Pays : United States

Commentaires et corrections

Type : UpdateIn

Déclaration de conflit d'intérêts

Competing interests: D.P., Ad.M., F.Z., M.G., C.S.F., J.B., C.S., H.V.D., K.H., W.R., M.A.S., G.Sc., B.G., F.B., J.d.I., A.R., J.Z., N.F., H.K., M.M.R, J.N., F.A.L., G.S., L.P., H.W.V., A.L., M.S.P. and D.C. are employees of Vir Biotechnology Inc. and may hold shares in Vir Biotechnology Inc. L.A.P. is a former employee and shareholder in Regeneron Pharmaceuticals. Regeneron provided no funding for this work. H.W.V. is a founder and holds shares in PierianDx and Casma Therapeutics. Neither company provided resources. D.C. is currently listed as an inventor on multiple patent applications, which disclose the subject matter described in this manuscript. The Veesler laboratory has received a sponsored research agreement from Vir Biotechnology Inc. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Young-Jun Park (YJ)

Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.

Dora Pinto (D)

Humabs Biomed SA, a subsidiary of Vir Biotechnology, 6500 Bellinzona, Switzerland.

Alexandra C Walls (AC)

Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.

Zhuoming Liu (Z)

Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Anna De Marco (A)

Humabs Biomed SA, a subsidiary of Vir Biotechnology, 6500 Bellinzona, Switzerland.

Fabio Benigni (F)

Humabs Biomed SA, a subsidiary of Vir Biotechnology, 6500 Bellinzona, Switzerland.

Fabrizia Zatta (F)

Humabs Biomed SA, a subsidiary of Vir Biotechnology, 6500 Bellinzona, Switzerland.

Chiara Silacci-Fregni (C)

Humabs Biomed SA, a subsidiary of Vir Biotechnology, 6500 Bellinzona, Switzerland.

Jessica Bassi (J)

Humabs Biomed SA, a subsidiary of Vir Biotechnology, 6500 Bellinzona, Switzerland.

Kaitlin R Sprouse (KR)

Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.

Amin Addetia (A)

Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.

John E Bowen (JE)

Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.

Cameron Stewart (C)

Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.

Martina Giurdanella (M)

Humabs Biomed SA, a subsidiary of Vir Biotechnology, 6500 Bellinzona, Switzerland.

Christian Saliba (C)

Humabs Biomed SA, a subsidiary of Vir Biotechnology, 6500 Bellinzona, Switzerland.

Barbara Guarino (B)

Humabs Biomed SA, a subsidiary of Vir Biotechnology, 6500 Bellinzona, Switzerland.

Michael A Schmid (MA)

Humabs Biomed SA, a subsidiary of Vir Biotechnology, 6500 Bellinzona, Switzerland.

Nicholas Franko (N)

Division of Allergy and Infectious Diseases, University of Washington, Seattle, WA 98195, USA.

Jennifer Logue (J)

Division of Allergy and Infectious Diseases, University of Washington, Seattle, WA 98195, USA.

Ha V Dang (HV)

Vir Biotechnology, San Francisco, CA 94158, USA.

Kevin Hauser (K)

Vir Biotechnology, San Francisco, CA 94158, USA.

Julia di Iulio (JD)

Vir Biotechnology, San Francisco, CA 94158, USA.

William Rivera (W)

Vir Biotechnology, San Francisco, CA 94158, USA.

Gretja Schnell (G)

Vir Biotechnology, San Francisco, CA 94158, USA.

Anushka Rajesh (A)

Vir Biotechnology, San Francisco, CA 94158, USA.

Jiayi Zhou (J)

Vir Biotechnology, San Francisco, CA 94158, USA.

Nisar Farhat (N)

Vir Biotechnology, San Francisco, CA 94158, USA.

Hannah Kaiser (H)

Vir Biotechnology, San Francisco, CA 94158, USA.

Martin Montiel-Ruiz (M)

Vir Biotechnology, San Francisco, CA 94158, USA.

Julia Noack (J)

Vir Biotechnology, San Francisco, CA 94158, USA.

Florian A Lempp (FA)

Vir Biotechnology, San Francisco, CA 94158, USA.

Javier Janer (J)

Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Rana Abdelnabi (R)

Laboratory of Virology and Chemotherapy, Rega Institute for Medical Research, KU Leuven, 3000 Leuven, Belgium.

Piet Maes (P)

Laboratory of Virology and Chemotherapy, Rega Institute for Medical Research, KU Leuven, 3000 Leuven, Belgium.

Paolo Ferrari (P)

Faculty of Biomedical Sciences, Università della Svizzera italiana, Lugano, Switzerland.
Division of Nephrology, Ente Ospedaliero Cantonale, Lugano, Switzerland.
Clinical School, University of New South Wales, Sydney, New South Wales, Australia.

Alessandro Ceschi (A)

Faculty of Biomedical Sciences, Università della Svizzera italiana, Lugano, Switzerland.
Clinical Trial Unit, Ente Ospedaliero Cantonale, Lugano, Switzerland.
Division of Clinical Pharmacology and Toxicology, Institute of Pharmacological Sciences of Southern Switzerland, Ente Ospedaliero Cantonale, Lugano, Switzerland.
Department of Clinical Pharmacology and Toxicology, University Hospital Zurich, Zurich, Switzerland.

Olivier Giannini (O)

Faculty of Biomedical Sciences, Università della Svizzera italiana, Lugano, Switzerland.
Department of Medicine, Ente Ospedaliero Cantonale, Bellinzona, Switzerland.

Guilherme Dias de Melo (GD)

Institut Pasteur, Université de Paris Cité, Lyssavirus Epidemiology and Neuropathology Unit, Paris, F-75015, France.

Lauriane Kergoat (L)

Institut Pasteur, Université de Paris Cité, Lyssavirus Epidemiology and Neuropathology Unit, Paris, F-75015, France.

Hervé Bourhy (H)

Institut Pasteur, Université de Paris Cité, Lyssavirus Epidemiology and Neuropathology Unit, Paris, F-75015, France.

Johan Neyts (J)

Laboratory of Virology and Chemotherapy, Rega Institute for Medical Research, KU Leuven, 3000 Leuven, Belgium.

Leah Soriaga (L)

Vir Biotechnology, San Francisco, CA 94158, USA.

Lisa A Purcell (LA)

Vir Biotechnology, San Francisco, CA 94158, USA.

Gyorgy Snell (G)

Vir Biotechnology, San Francisco, CA 94158, USA.

Sean P J Whelan (SPJ)

Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Antonio Lanzavecchia (A)

Humabs Biomed SA, a subsidiary of Vir Biotechnology, 6500 Bellinzona, Switzerland.

Herbert W Virgin (HW)

Vir Biotechnology, San Francisco, CA 94158, USA.
Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO, USA.
Department of Internal Medicine, UT Southwestern Medical Center, Dallas, TX, USA.

Luca Piccoli (L)

Humabs Biomed SA, a subsidiary of Vir Biotechnology, 6500 Bellinzona, Switzerland.

Helen Chu (H)

Division of Allergy and Infectious Diseases, University of Washington, Seattle, WA 98195, USA.

Matteo Samuele Pizzuto (MS)

Humabs Biomed SA, a subsidiary of Vir Biotechnology, 6500 Bellinzona, Switzerland.

Davide Corti (D)

Humabs Biomed SA, a subsidiary of Vir Biotechnology, 6500 Bellinzona, Switzerland.

David Veesler (D)

Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.

Classifications MeSH