Chromatin extracted from common carp testis as an economical and easily available adsorbent for ethidium bromide decontamination.
Chromatin
Decontamination
Ethidium bromide
Journal
Heliyon
ISSN: 2405-8440
Titre abrégé: Heliyon
Pays: England
ID NLM: 101672560
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
received:
25
01
2022
revised:
22
03
2022
accepted:
24
05
2022
entrez:
9
6
2022
pubmed:
10
6
2022
medline:
10
6
2022
Statut:
epublish
Résumé
The waste of ethidium bromide (EtBr) used in the laboratory will bring a great burden to the environment, which need to be solved urgently. In the present paper, an efficient and inexpensive method for EtBr removal using chromatin extracted from common carp testis was investigated. The observation of fluorescence microscopy showed that chromatin had similar property to DNA for selective adsorption of EtBr. The results of batch adsorption showed that the removal efficiency of EtBr by chromatin exceeded 99% at pH 7.4 and 30 °C for 3 min with the EtBr concentration of 2 mg L
Identifiants
pubmed: 35677409
doi: 10.1016/j.heliyon.2022.e09565
pii: S2405-8440(22)00853-2
pmc: PMC9167975
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e09565Informations de copyright
© 2022 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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