Homoharringtonine is a transdermal granular permeation enhancer.

Animals Claudin-1 / metabolism Claudin-4 / metabolism Dextrans / metabolism Homoharringtonine / pharmacology Keratinocytes / drug effects Mice Tight Junctions / metabolism
Permeation enhancer Stratum granulosum Tight junction Transdermal absorption

Journal

Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516

Informations de publication

Date de publication:
06 08 2022
Historique:
received: 05 04 2022
revised: 12 04 2022
accepted: 14 04 2022
pubmed: 10 6 2022
medline: 18 6 2022
entrez: 9 6 2022
Statut: ppublish

Résumé

Although modulation of claudin-1-based tight junction (TJ) in stratum granulosum is an option for transdermal absorption of drugs, granular permeation enhancers have never been developed. We previously found that homoharringtonine (HHT), a natural alkanoid, weakened intestinal epithelial barrier with changing expression and cellular localization of TJ components such as claudin-1 and claudin-4. In the present study, we investigated whether HHT is an epidermal granular permeation enhancer. Treatment of normal human epidermal keratinocytes (NHEK) cells with HHT decreased claudin-1 and claudin-4 but not zonula occludens-1 and E-cadherin. HHT lowered TJ-integrity in NHEK cells, accompanied by permeation-enhancement of dextran (4 kDa) in a dose-dependent manner. Transdermal treatment of mice with HHT weakened epidermal barrier. HHT treatment enhanced transdermal absorption of dextran with a molecular mass of up to 10 kDa. Together, HHT may be a transdermal absorption enhancer.

Identifiants

DOI: 10.1016/j.bbrc.2022.04.067 PMID: 35679696
pubmed: 35679696
pii: S0006-291X(22)00596-4
doi: 10.1016/j.bbrc.2022.04.067
pii:
doi:

Substances chimiques

Claudin-1 0
Claudin-4 0
Dextrans 0
Homoharringtonine 6FG8041S5B

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

140-144

Informations de copyright

Copyright © 2022. Published by Elsevier Inc.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors have no conflicts of interest to declare.

Auteurs

Akihiro Watari (A)

Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan; Department of Medical Innovation, Osaka University Hospital, Osaka, Japan. Electronic address: akihiro.watari@dmi.med.osaka-u.ac.jp.

Kana Fujiwara (K)

Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.

Kiyohito Yagi (K)

Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.

Keisuke Tachibana (K)

Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.

Takeshi Katsurada (T)

Faculty of Pharmaceutical Sciences, Osaka University, Osaka, Japan.

Akira Myoui (A)

Department of Medical Innovation, Osaka University Hospital, Osaka, Japan.

Masuo Kondoh (M)

Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan. Electronic address: masuo@phs.osaka-u.ac.jp.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Homoharringtonine is a transdermal granular...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de la jonction serrée › Claudines › Claudine-1 › Homoharringtonine is a transdermal granular...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de la jonction serrée › Claudines › Claudine-4 › Homoharringtonine is a transdermal granular...
questionsmedicales.fr › Glucides › Polyosides › Glucanes › Dextrane › Homoharringtonine is a transdermal granular...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérocycliques avec 4 noyaux ou plus › Harringtonines › Homoharringtonine › Homoharringtonine is a transdermal granular...
questionsmedicales.fr › Cellules › Cellules épithéliales › Kératinocytes › Homoharringtonine is a transdermal granular...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Homoharringtonine is a transdermal granular...
questionsmedicales.fr › Cellules › Structures cellulaires › Membrane cellulaire › Structures de la membrane cellulaire › Jonctions intercellulaires › Jonctions serrées › Homoharringtonine is a transdermal granular...