R-SPONDIN2

Humans Lung Mesenchymal Stem Cells Organogenesis Organoids Wnt Signaling Pathway
R-Spondin2 RSPO2 bud tip progenitor human development lung development lung mesenchyme lung organoid mesenchymal cell single-cell RNA-seq stem cell niche

Journal

Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028

Informations de publication

Date de publication:
11 07 2022
Historique:
received: 22 03 2021
revised: 18 04 2022
accepted: 16 05 2022
pubmed: 10 6 2022
medline: 15 7 2022
entrez: 9 6 2022
Statut: ppublish

Résumé

The human respiratory epithelium is derived from a progenitor cell in the distal buds of the developing lung. These "bud tip progenitors" are regulated by reciprocal signaling with surrounding mesenchyme; however, mesenchymal heterogeneity and function in the developing human lung are poorly understood. We interrogated single-cell RNA sequencing data from multiple human lung specimens and identified a mesenchymal cell population present during development that is highly enriched for expression of the WNT agonist RSPO2, and we found that the adjacent bud tip progenitors are enriched for the RSPO2 receptor LGR5. Functional experiments using organoid models, explant cultures, and FACS-isolated RSPO2

Identifiants

DOI: 10.1016/j.devcel.2022.05.010 PMID: 35679862 PMC: PMC9283295
pubmed: 35679862
pii: S1534-5807(22)00367-7
doi: 10.1016/j.devcel.2022.05.010
pmc: PMC9283295
mid: NIHMS1810736
pii:
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1598-1614.e8

Subventions

Organisme : NIDDK NIH HHS
ID : DP2 DK128801
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL119215
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM145470
Pays : United States
Organisme : NHLBI NIH HHS
ID : F31 HL152531
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM145304
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007863
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG067014
Pays : United States
Organisme : NICHD NIH HHS
ID : R24 HD000836
Pays : United States
Organisme : NHLBI NIH HHS
ID : F31 HL142197
Pays : United States
Organisme : NIDCR NIH HHS
ID : T32 DE007057
Pays : United States
Organisme : NHLBI NIH HHS
ID : F31 HL146162
Pays : United States

Informations de copyright

Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

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Auteurs

Renee F C Hein (RFC)

Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Joshua H Wu (JH)

Department of Internal Medicine, Gastroenterology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Emily M Holloway (EM)

Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Tristan Frum (T)

Department of Internal Medicine, Gastroenterology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Ansley S Conchola (AS)

Program in Cell and Molecular Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Yu-Hwai Tsai (YH)

Department of Internal Medicine, Gastroenterology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Angeline Wu (A)

Department of Internal Medicine, Gastroenterology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Alexis S Fine (AS)

Department of Internal Medicine, Gastroenterology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Alyssa J Miller (AJ)

Program in Cell and Molecular Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Emmanuelle Szenker-Ravi (E)

Laboratory of Human Genetics & Therapeutics, Genome Institute of Singapore, A(∗)STAR, Singapore 138648, Singapore.

Kelley S Yan (KS)

Columbia Center for Human Development, Columbia Stem Cell Initiative, Departments of Medicine and Genetics and Development, Columbia University Irving Medical Center, New York, NY 10032, USA.

Calvin J Kuo (CJ)

Department of Medicine, Division of Hematology, Stanford University School of Medicine, Stanford, CA 94305, USA.

Ian Glass (I)

Department of Pediatrics, Genetic Medicine, University of Washington, Seattle, WA 98195, USA.

Bruno Reversade (B)

Laboratory of Human Genetics & Therapeutics, Genome Institute of Singapore, A(∗)STAR, Singapore 138648, Singapore; Laboratory of Human Genetics & Therapeutics, Institute of Molecular and Cell Biology (IMCB), A∗STAR, Singapore; Medical Genetics Department, Koç University School of Medicine (KUSOM), Istanbul, Turkey.

Jason R Spence (JR)

Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Department of Internal Medicine, Gastroenterology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Program in Cell and Molecular Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Department of Biomedical Engineering, University of Michigan College of Engineering, Ann Arbor, MI 48109, USA. Electronic address: spencejr@umich.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › R-SPONDIN2
questionsmedicales.fr › Appareil respiratoire › Poumon › R-SPONDIN2
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches multipotentes › Cellules souches mésenchymateuses › R-SPONDIN2
questionsmedicales.fr › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Reproduction › Développement embryonnaire et foetal › Organogenèse › R-SPONDIN2
questionsmedicales.fr › Tissus › Organoïdes › R-SPONDIN2
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Transduction du signal › Voie de signalisation Wnt › R-SPONDIN2