Distinct dissociation rates of murine and human norovirus P-domain dimers suggest a role of dimer stability in virus-host interactions.


Journal

Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179

Informations de publication

Date de publication:
09 06 2022
Historique:
received: 19 03 2021
accepted: 19 05 2022
entrez: 10 6 2022
pubmed: 11 6 2022
medline: 14 6 2022
Statut: epublish

Résumé

Norovirus capsids are icosahedral particles composed of 90 dimers of the major capsid protein VP1. The C-terminus of the VP1 proteins forms a protruding (P)-domain, mediating receptor attachment, and providing a target for neutralizing antibodies. NMR and native mass spectrometry directly detect P-domain monomers in solution for murine (MNV) but not for human norovirus (HuNoV). We report that the binding of glycochenodeoxycholic acid (GCDCA) stabilizes MNV-1 P-domain dimers (P-dimers) and induces long-range NMR chemical shift perturbations (CSPs) within loops involved in antibody and receptor binding, likely reflecting corresponding conformational changes. Global line shape analysis of monomer and dimer cross-peaks in concentration-dependent methyl TROSY NMR spectra yields a dissociation rate constant k

Identifiants

pubmed: 35680964
doi: 10.1038/s42003-022-03497-4
pii: 10.1038/s42003-022-03497-4
pmc: PMC9184547
doi:

Substances chimiques

Capsid Proteins 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

563

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI141465
Pays : United States

Informations de copyright

© 2022. The Author(s).

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Auteurs

Robert Creutznacher (R)

University of Lübeck, Center of Structural and Cell Biology in Medicine (CSCM), Institute of Chemistry and Metabolomics, Ratzeburger Allee 160, 23562, Lübeck, Germany.
Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL, Utrecht, The Netherlands.

Thorben Maass (T)

University of Lübeck, Center of Structural and Cell Biology in Medicine (CSCM), Institute of Chemistry and Metabolomics, Ratzeburger Allee 160, 23562, Lübeck, Germany.

Jasmin Dülfer (J)

Leibniz Institute of Virology (LIV), Martinistrasse 52, 20251, Hamburg, Germany.

Clara Feldmann (C)

University of Lübeck, Center of Structural and Cell Biology in Medicine (CSCM), Institute of Chemistry and Metabolomics, Ratzeburger Allee 160, 23562, Lübeck, Germany.

Veronika Hartmann (V)

University of Lübeck, Center of Structural and Cell Biology in Medicine (CSCM), Institute of Virology and Cell Biology, Ratzeburger Allee 160, 23562, Lübeck, Germany.

Miranda Sophie Lane (MS)

University of Lübeck, Center of Structural and Cell Biology in Medicine (CSCM), Institute of Virology and Cell Biology, Ratzeburger Allee 160, 23562, Lübeck, Germany.

Jan Knickmann (J)

University of Lübeck, Center of Structural and Cell Biology in Medicine (CSCM), Institute of Virology and Cell Biology, Ratzeburger Allee 160, 23562, Lübeck, Germany.

Leon Torben Westermann (LT)

University of Lübeck, Center of Structural and Cell Biology in Medicine (CSCM), Institute of Chemistry and Metabolomics, Ratzeburger Allee 160, 23562, Lübeck, Germany.

Lars Thiede (L)

Leibniz Institute of Virology (LIV), Martinistrasse 52, 20251, Hamburg, Germany.
CSSB Centre for Structural Systems Biology, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607, Hamburg, Germany.

Thomas J Smith (TJ)

University of Texas Medical Branch at Galveston, Department of Biochemistry and Molecular Biology, 301 University Boulevard, Route 0645, Galveston, TX, 77555, USA.

Charlotte Uetrecht (C)

Leibniz Institute of Virology (LIV), Martinistrasse 52, 20251, Hamburg, Germany.
CSSB Centre for Structural Systems Biology, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607, Hamburg, Germany.
School of Life Sciences, University of Siegen, 57076, Siegen, Germany.

Alvaro Mallagaray (A)

University of Lübeck, Center of Structural and Cell Biology in Medicine (CSCM), Institute of Chemistry and Metabolomics, Ratzeburger Allee 160, 23562, Lübeck, Germany.

Christopher A Waudby (CA)

Institute of Structural and Molecular Biology, University College London, Gower Street, London, WC1E 6BT, UK.

Stefan Taube (S)

University of Lübeck, Center of Structural and Cell Biology in Medicine (CSCM), Institute of Virology and Cell Biology, Ratzeburger Allee 160, 23562, Lübeck, Germany. stefan.taube@uni-luebeck.de.

Thomas Peters (T)

University of Lübeck, Center of Structural and Cell Biology in Medicine (CSCM), Institute of Chemistry and Metabolomics, Ratzeburger Allee 160, 23562, Lübeck, Germany. thomas.peters@uni-luebeck.de.

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