The Activity of Plant-Derived Ren's Oligopeptides-1 against the Pseudorabies Virus.
RTCA
Ren’s oligopeptides-1
high content screening
pseudorabies virus (PRV)
Journal
Animals : an open access journal from MDPI
ISSN: 2076-2615
Titre abrégé: Animals (Basel)
Pays: Switzerland
ID NLM: 101635614
Informations de publication
Date de publication:
25 May 2022
25 May 2022
Historique:
received:
08
03
2022
revised:
21
05
2022
accepted:
22
05
2022
entrez:
10
6
2022
pubmed:
11
6
2022
medline:
11
6
2022
Statut:
epublish
Résumé
Newly synthesized Ren's oligopeptides-1 was found to have an antiviral effect in clinical trials, and the purpose of this study was to further demonstrate the antiviral activity of Ren's oligopeptides-1 against the PRV 152-GFP strain. We used the real-time cell analysis system (RTCA) to detect the cytotoxicity of different concentrations of Ren's oligopeptides-1. We then applied high content screening (HCS) to detect the antiviral activity of Ren's oligopeptides-1 against PRV. Meanwhile, the fluorescence signal of the virus was collected in real time and the expression levels of the related genes in the PK15 cells infected with PRV were detected using real-time PCR. At the mRNA level, we discovered that, at a concentration of 6 mg/mL, Ren's oligopeptides-1 reduced the expression of pseudorabies virus (PRV) genes such as IE180, UL18, UL54, and UL21 at a concentration of 6 mg/mL. We then determined that Ren's oligopeptides-1 has an EC
Identifiants
pubmed: 35681806
pii: ani12111341
doi: 10.3390/ani12111341
pmc: PMC9179334
pii:
doi:
Types de publication
Journal Article
Langues
eng
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