Oral D-Aspartate Treatment Improves Sperm Fertility in Both Young and Adult B6N Mice.
3R
D-Aspartate
in vitro fertilization (IVF)
sperm fertility
spermatozoa
Journal
Animals : an open access journal from MDPI
ISSN: 2076-2615
Titre abrégé: Animals (Basel)
Pays: Switzerland
ID NLM: 101635614
Informations de publication
Date de publication:
25 May 2022
25 May 2022
Historique:
received:
23
03
2022
revised:
13
05
2022
accepted:
23
05
2022
entrez:
10
6
2022
pubmed:
11
6
2022
medline:
11
6
2022
Statut:
epublish
Résumé
D-Aspartate (D-Asp) treatment improved the fertility of young male C57BL/6N mice in vivo revealing a direct role on capacitation, acrosome reaction, and fertility in vitro in young males only. We investigated whether the positive effect of D-Asp on fertility could be extended to adult males and evaluated the efficacy of a 2- or 4-week-treatment in vivo. Therefore, 20 mM sodium D-Asp was supplied in drinking water to males of different ages so that they were 9 or 16 weeks old at the end of the experiments. After sperm freezing, the in vitro fertilization (IVF) rate, the birth rate, hormone levels (luteinizing hormone (LH), epitestosterone, and testosterone), the sperm quality (morphology, abnormalities, motility, and velocity), the capacitation rate, and the acrosome reaction were investigated. Oral D-Asp treatment improves the fertilizing capability in mice regardless of the age of the animals. Importantly, a short D-Asp treatment of 2 weeks in young males elevates sperm parameters to the levels of untreated adult animals. In vivo, D-Asp treatment highly improves sperm quality but not sperm concentration. Therefore, D-Asp plays a beneficial role in mouse male fertility and may be highly relevant for cryorepositories to improve mouse sperm biobanking.
Identifiants
pubmed: 35681815
pii: ani12111350
doi: 10.3390/ani12111350
pmc: PMC9179375
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Infrafrontier-I3 project under EU contract Grant Agreement Number 312325 of the EC FP7 Capacities Specific Programme at CNR, Institute of Biochemistry and Cellular Biology and intramural funding to EM.
ID : Grant Agreement Number 312325
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