A New Strategy for High-Efficient Tandem Enrichment and Simultaneous Profiling of N-Glycopeptides and Phosphopeptides in Lung Cancer Tissue.
N-glycopeptides
enrichment
mass spectrometry
phosphopeptides
proteomics
Journal
Frontiers in molecular biosciences
ISSN: 2296-889X
Titre abrégé: Front Mol Biosci
Pays: Switzerland
ID NLM: 101653173
Informations de publication
Date de publication:
2022
2022
Historique:
received:
19
04
2022
accepted:
04
05
2022
entrez:
10
6
2022
pubmed:
11
6
2022
medline:
11
6
2022
Statut:
epublish
Résumé
N-glycosylation and phosphorylation, two common posttranslational modifications, play important roles in various biological processes and are extensively studied for biomarker and drug target screening. Because of their low abundance, enrichment of N-glycopeptides and phosphopeptides prior to LC-MS/MS analysis is essential. However, simultaneous characterization of these two types of posttranslational modifications in complex biological samples is still challenging, especially for tiny amount of samples obtained in tissue biopsy. Here, we introduced a new strategy for the highly efficient tandem enrichment of N-glycopeptides and phosphopeptides using HILIC and TiO
Identifiants
pubmed: 35685241
doi: 10.3389/fmolb.2022.923363
pii: 923363
pmc: PMC9171396
doi:
Types de publication
Journal Article
Langues
eng
Pagination
923363Informations de copyright
Copyright © 2022 Du, Yang, Liu, Chen, Zhao, Bai, Shao, Zhang and Qin.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
J Chromatogr A. 2018 Mar 9;1540:87-93
pubmed: 29429745
Anal Chim Acta. 2020 Feb 15;1098:181-189
pubmed: 31948582
Sci Adv. 2020 Oct 2;6(40):
pubmed: 33008897
Angew Chem Int Ed Engl. 2015 Aug 24;54(35):10211-5
pubmed: 26179149
Anal Chim Acta. 2020 May 15;1111:103-113
pubmed: 32312387
Cell. 2012 Jul 20;150(2):413-25
pubmed: 22817900
Anal Chem. 2022 Mar 15;94(10):4155-4164
pubmed: 35239328
Cell Mol Life Sci. 2021 Mar;78(5):2231-2245
pubmed: 32926180
Ann Surg Oncol. 2008 Mar;15(3):889-99
pubmed: 18181001
Lab Invest. 2015 Jan;95(1):14-25
pubmed: 25365205
Anal Chem. 2019 Sep 17;91(18):11589-11597
pubmed: 31398006
Chem Biol. 2014 Jan 16;21(1):1-15
pubmed: 24439204
Science. 2019 Oct 11;366(6462):
pubmed: 31601740
Talanta. 2017 Aug 15;171:124-131
pubmed: 28551118
Nature. 2003 Mar 13;422(6928):198-207
pubmed: 12634793
Nat Commun. 2021 May 11;12(1):2672
pubmed: 33976130
Mol Cell Proteomics. 2018 Sep;17(9):1850-1863
pubmed: 29848782
Methods Mol Biol. 2021;2228:185-203
pubmed: 33950492
J Alzheimers Dis. 2021;84(3):937-957
pubmed: 34602469
Anal Chem. 2010 Apr 1;82(7):2907-15
pubmed: 20199055
Cell Rep. 2018 May 29;23(9):2819-2831.e5
pubmed: 29847809
Cell Metab. 2013 Feb 5;17(2):291-302
pubmed: 23395175
J Chromatogr A. 2017 Jul 7;1505:56-62
pubmed: 28533032
Talanta. 2018 Feb 1;178:274-281
pubmed: 29136822
FEBS J. 2010 Jan;277(1):95-105
pubmed: 19919546
Mass Spectrom Rev. 2021 Jul;40(4):309-333
pubmed: 32491218
J Histochem Cytochem. 2014 May;62(5):335-46
pubmed: 24487999
Nat Chem Biol. 2016 Nov;12(11):959-966
pubmed: 27642862
Sci Rep. 2018 Apr 25;8(1):6509
pubmed: 29695737
Cell. 2010 May 28;141(5):897-907
pubmed: 20510933
Anal Chem. 2016 Jan 19;88(2):1447-54
pubmed: 26684413
Nat Methods. 2009 May;6(5):359-62
pubmed: 19377485
Proteomics Clin Appl. 2015 Apr;9(3-4):383-95
pubmed: 25488289
J Proteome Res. 2021 Jun 4;20(6):3278-3289
pubmed: 33929864
Mass Spectrom Rev. 2015 Mar-Apr;34(2):148-65
pubmed: 24889823
Otolaryngol Head Neck Surg. 2012 Feb;146(2):250-8
pubmed: 22041223
Cell. 2020 Dec 10;183(6):1699-1713.e13
pubmed: 33188775
Mikrochim Acta. 2019 Aug 3;186(9):600
pubmed: 31377859
Anal Chim Acta. 2022 Mar 15;1198:339552
pubmed: 35190121
J Proteome Res. 2019 Jan 4;18(1):359-371
pubmed: 30370771
Mol Cell Proteomics. 2021;20:100112
pubmed: 34129940