Multi-dimensional Fluorescence Live-Cell Imaging for Glucosome Dynamics in Living Human Cells.
Cancer Metabolism
Fluorescence Microscopy
Glucose Metabolism
Glucosome
Glycolysis
Lattice Light-sheet Microscopy
Live-cell Imaging
Macromolecular Assembly
Metabolic Complex
Metabolic Condensate
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
10
6
2022
pubmed:
11
6
2022
medline:
15
6
2022
Statut:
ppublish
Résumé
Fluorescence live-cell imaging that has contributed to our understanding of cell biology is now at the frontline of studying quantitative biochemistry in a cell. Particularly, technological advancements of fluorescence live-cell imaging and associated strategies in recent years have allowed us to discover various subcellular macromolecular assemblies in living human cells. Here we describe how real-time dynamics of a multienzyme metabolic assembly, the "glucosome," that is responsible for regulating glucose flux at subcellular levels, has been investigated in both 2- and 3-dimensional space of single human cells. We envision that such multi-dimensional fluorescence live-cell imaging will continue to revolutionize our understanding of how intracellular metabolic pathways and their network are functionally orchestrated at single-cell levels.
Identifiants
pubmed: 35687227
doi: 10.1007/978-1-0716-2269-8_2
pmc: PMC9191769
mid: NIHMS1747605
doi:
Substances chimiques
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
15-26Subventions
Organisme : NIGMS NIH HHS
ID : T32 GM066706
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM125981
Pays : United States
Organisme : NIGMS NIH HHS
ID : R25 GM055036
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM134086
Pays : United States
Organisme : NCI NIH HHS
ID : R03 CA219609
Pays : United States
Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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