Isolation and comparison of neural stem cells from the adult rat brain and spinal cord canonical neurogenic niches.

Animals Brain Neural Stem Cells Neurogenesis Rats Spinal Cord Injuries
Cell Biology Cell culture Cell isolation Microscopy Model Organisms Neuroscience Stem Cells

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
17 06 2022
Historique:
entrez: 13 6 2022
pubmed: 14 6 2022
medline: 15 6 2022
Statut: epublish

Résumé

Here, we present a unified protocol for the extraction, culture, and basic characterization of rat neural stem cells (NSCs) from all three canonical neurogenic niches in the brain and spinal cord. We describe tissue dissection and dissociation, cell culture, followed by EdU labeling and characterization of NSCs. By yielding considerable numbers of viable cells per animal, this protocol enables the establishment of substantial, long-term cell banks, thus offering cost and labor efficiency while significantly reducing the numbers of animals used.

Identifiants

DOI: 10.1016/j.xpro.2022.101426 PMID: 35693206 PMC: PMC9184809
pubmed: 35693206
doi: 10.1016/j.xpro.2022.101426
pii: S2666-1667(22)00306-9
pmc: PMC9184809
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101426

Informations de copyright

© 2022 The Author(s).

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Références

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Auteurs

Lars Erik Schiro (LE)

Department of Neuromedicine and Movement Science, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology (NTNU), 7030 Trondheim, Norway.

Ulrich Stefan Bauer (US)

Department of Neuromedicine and Movement Science, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology (NTNU), 7030 Trondheim, Norway.

Axel Sandvig (A)

Department of Neuromedicine and Movement Science, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology (NTNU), 7030 Trondheim, Norway.
Department of Clinical Neuroscience, Division of Neuro, Head, and Neck, Umeå University Hospital, 907 37 Umeå, Sweden.
Department of Community and Rehabilitation, Division of Neuro, Head and Neck, Umeå University Hospital, 907 37 Umeå, Sweden.

Ioanna Sandvig (I)

Department of Neuromedicine and Movement Science, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology (NTNU), 7030 Trondheim, Norway.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Isolation and comparison of neural stem cells...
questionsmedicales.fr › Système nerveux › Système nerveux central › Encéphale › Isolation and comparison of neural stem cells...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches neurales › Isolation and comparison of neural stem cells...
questionsmedicales.fr › Phénomènes physiologiques de l'appareil locomoteur et du système nerveux › Phénomènes physiologiques du système nerveux › Neurogenèse › Isolation and comparison of neural stem cells...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Isolation and comparison of neural stem cells...
questionsmedicales.fr › Plaies et blessures › Traumatismes de la moelle épinière › Isolation and comparison of neural stem cells...