Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue.
Cell Biology
Genomics
Health Sciences
RNAseq
Sequencing
Single Cell
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
17 06 2022
17 06 2022
Historique:
entrez:
13
6
2022
pubmed:
14
6
2022
medline:
15
6
2022
Statut:
epublish
Résumé
Concomitant profiling of transcriptome and chromatin accessibility in isolated nuclei can reveal gene regulatory control mechanisms in health and disease. We report a single nucleus multi-omics analysis protocol optimized for frozen archived postmortem human pituitaries that is also effective for frozen ovine and murine pituitaries and human skeletal muscle biopsies. Its main advantages are that (1) it is not limited to fresh tissue, (2) it avoids tissue dissociation-induced transcriptional changes, and (3) it includes a novel, automated quality control pipeline. For complete details on the use and execution of this protocol, please refer to Ruf-Zamojski et al. (2021) and Zhang et al. (2022).
Identifiants
pubmed: 35693209
doi: 10.1016/j.xpro.2022.101446
pii: S2666-1667(22)00326-4
pmc: PMC9184808
doi:
Substances chimiques
Chromatin
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
101446Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK046943
Pays : United States
Informations de copyright
© 2022 The Authors.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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