Deletion of RBP-Jkappa gene in mesenchymal cells causes rickets like symptoms in the mouse.
Bone
Cartilage
Notch
Rickets
Wnt5a
Journal
Current medicine (Cham, Switzerland)
ISSN: 2731-0868
Titre abrégé: Curr Med (Cham)
Pays: Switzerland
ID NLM: 9918402367906676
Informations de publication
Date de publication:
2022
2022
Historique:
received:
08
03
2022
accepted:
26
04
2022
entrez:
13
6
2022
pubmed:
14
6
2022
medline:
14
6
2022
Statut:
ppublish
Résumé
Crosstalk between different signalling pathways provide deep insights for how molecules play synergistic roles in developmental and pathological conditions. RBP-Jkappa is the key effector of the canonical Notch pathway. Previously we have identified that Wnt5a, a conventional non-canonical Wnt pathway member, was under the direct transcriptional control of RBP-Jkappa in dermal papilla cells. In this study we further extended this regulation axis to the other two kind of skeletal cells: chondrocytes and osteoblasts. Mice with conditional mesenchymal deletion of RBP-Jkappa developed Rickets like symptoms. Molecular analysis suggested local defects of Wnt5a expression in chondrocytes and osteoblasts at both mRNA and protein levels, which impeded chondrocyte and osteoblast differentiation. The defects existing in the RBP-Jkappa deficient mutants could be rescued by recombinant Wnt5a treatment at both cellular level and tissue/organ level. Our results therefore provide a model of studying the connection of Notch and Wnt5a pathways with Rickets. The online version contains supplementary material available at 10.1007/s44194-022-00007-w.
Identifiants
pubmed: 35694720
doi: 10.1007/s44194-022-00007-w
pii: 7
pmc: PMC9177048
doi:
Types de publication
Journal Article
Langues
eng
Pagination
7Informations de copyright
© The Author(s) 2022.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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