Integrated multi-omics reveal polycomb repressive complex 2 restricts human trophoblast induction.

Cell Differentiation / genetics Chromatin / genetics Histones / genetics Humans Pluripotent Stem Cells Polycomb Repressive Complex 2 / genetics Trophoblasts / metabolism

Journal

Nature cell biology
ISSN: 1476-4679
Titre abrégé: Nat Cell Biol
Pays: England
ID NLM: 100890575

Informations de publication

Date de publication:
06 2022
Historique:
received: 02 09 2021
accepted: 05 05 2022
pubmed: 14 6 2022
medline: 22 6 2022
entrez: 13 6 2022
Statut: ppublish

Résumé

Human naive pluripotent stem cells have unrestricted lineage potential. Underpinning this property, naive cells are thought to lack chromatin-based lineage barriers. However, this assumption has not been tested. Here we define the chromatin-associated proteome, histone post-translational modifications and transcriptome of human naive and primed pluripotent stem cells. Our integrated analysis reveals differences in the relative abundance and activities of distinct chromatin modules. We identify a strong enrichment of polycomb repressive complex 2 (PRC2)-associated H3K27me3 in the chromatin of naive pluripotent stem cells and H3K27me3 enrichment at promoters of lineage-determining genes, including trophoblast regulators. PRC2 activity acts as a chromatin barrier restricting the differentiation of naive cells towards the trophoblast lineage, whereas inhibition of PRC2 promotes trophoblast-fate induction and cavity formation in human blastoids. Together, our results establish that human naive pluripotent stem cells are not epigenetically unrestricted, but instead possess chromatin mechanisms that oppose the induction of alternative cell fates.

Identifiants

DOI: 10.1038/s41556-022-00932-w PMID: 35697783 PMC: PMC9203278
pubmed: 35697783
doi: 10.1038/s41556-022-00932-w
pii: 10.1038/s41556-022-00932-w
pmc: PMC9203278
doi:

Substances chimiques

Chromatin 0
Histones 0
Polycomb Repressive Complex 2 EC 2.1.1.43

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

858-871

Subventions

Organisme : Wellcome Trust
ID : 102160/Z/13/Z
Pays : United Kingdom
Organisme : NCI NIH HHS
ID : P01 CA196539
Pays : United States
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/B/000C0421
Pays : United Kingdom
Organisme : NIA NIH HHS
ID : P01 AG031862
Pays : United States
Organisme : Wellcome Trust
ID : 215116/Z/18/Z
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/B/000C0422
Pays : United Kingdom
Organisme : NIAID NIH HHS
ID : R01 AI118891
Pays : United States
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N018419/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/V02969X/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/J003808/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/T011769/1
Pays : United Kingdom

Commentaires et corrections

Type : CommentIn

Informations de copyright

© 2022. The Author(s).

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Auteurs

Dick W Zijlmans (DW)

Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences (RIMLS), Oncode Institute, Radboud University Nijmegen, Nijmegen, The Netherlands.

Irene Talon (I)

Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.
ORCID: 0000-0002-5800-5555

Sigrid Verhelst (S)

ProGenTomics, Laboratory of Pharmaceutical Biotechnology, Ghent University, Ghent, Belgium.
ORCID: 0000-0002-6791-3782

Adam Bendall (A)

Epigenetics Programme, Babraham Institute, Cambridge, UK.
ORCID: 0000-0002-6865-2625

Karlien Van Nerum (K)

Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.
ORCID: 0000-0002-8952-5473

Alok Javali (A)

Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria.

Andrew A Malcolm (AA)

Epigenetics Programme, Babraham Institute, Cambridge, UK.
The Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.
ORCID: 0000-0001-6240-7701

Sam S F A van Knippenberg (SSFA)

Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.
ORCID: 0000-0001-5656-3046

Laura Biggins (L)

Bioinformatics Group, Babraham Institute, Cambridge, UK.

San Kit To (SK)

Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.
ORCID: 0000-0002-3057-2902

Adrian Janiszewski (A)

Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.

Danielle Admiraal (D)

Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences (RIMLS), Radboud University Nijmegen, Nijmegen, The Netherlands.

Ruth Knops (R)

Laboratory of Hematology, Department of Laboratory Medicine, Radboud University Nijmegen Medical Centre (RadboudUMC), Nijmegen, the Netherlands.

Nikky Corthout (N)

VIB Center for Brain and Disease Research, KU Leuven, VIB Bioimaging Core, Leuven, Belgium.

Bradley P Balaton (BP)

Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.
ORCID: 0000-0002-5130-7868

Grigorios Georgolopoulos (G)

Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.

Amitesh Panda (A)

Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.
ORCID: 0000-0002-2702-3494

Natarajan V Bhanu (NV)

Epigenetics Institute, Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Amanda J Collier (AJ)

Epigenetics Programme, Babraham Institute, Cambridge, UK.

Charlene Fabian (C)

Epigenetics Programme, Babraham Institute, Cambridge, UK.

Ryan N Allsop (RN)

Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.

Joel Chappell (J)

Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.

Thi Xuan Ai Pham (TXA)

Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.
ORCID: 0000-0003-3866-2002

Michael Oberhuemer (M)

Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.
ORCID: 0000-0003-2273-2782

Cankat Ertekin (C)

Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.

Lotte Vanheer (L)

Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.

Paraskevi Athanasouli (P)

Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.

Frederic Lluis (F)

Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.

Dieter Deforce (D)

ProGenTomics, Laboratory of Pharmaceutical Biotechnology, Ghent University, Ghent, Belgium.
ORCID: 0000-0002-0635-661X

Joop H Jansen (JH)

Laboratory of Hematology, Department of Laboratory Medicine, Radboud University Nijmegen Medical Centre (RadboudUMC), Nijmegen, the Netherlands.

Benjamin A Garcia (BA)

Epigenetics Institute, Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Michiel Vermeulen (M)

Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences (RIMLS), Oncode Institute, Radboud University Nijmegen, Nijmegen, The Netherlands.
Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences (RIMLS), Radboud University Nijmegen, Nijmegen, The Netherlands.
ORCID: 0000-0003-0836-6894

Nicolas Rivron (N)

Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria.
ORCID: 0000-0003-1590-5964

Maarten Dhaenens (M)

ProGenTomics, Laboratory of Pharmaceutical Biotechnology, Ghent University, Ghent, Belgium. Maarten.Dhaenens@UGent.be.
ORCID: 0000-0002-9801-3509

Hendrik Marks (H)

Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences (RIMLS), Radboud University Nijmegen, Nijmegen, The Netherlands. Hendrik.Marks@ru.nl.
ORCID: 0000-0002-4198-3731

Peter J Rugg-Gunn (PJ)

Epigenetics Programme, Babraham Institute, Cambridge, UK. peter.rugg-gunn@babraham.ac.uk.
The Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. peter.rugg-gunn@babraham.ac.uk.
The Centre for Trophoblast Research, University of Cambridge, Cambridge, UK. peter.rugg-gunn@babraham.ac.uk.
ORCID: 0000-0002-9601-5949

Vincent Pasque (V)

Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium. vincent.pasque@kuleuven.be.
ORCID: 0000-0002-5129-0146

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Integrated multi-omics reveal polycomb...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Structures de chromosome › Chromatine › Integrated multi-omics reveal polycomb...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Histone › Integrated multi-omics reveal polycomb...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Integrated multi-omics reveal polycomb...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Integrated multi-omics reveal polycomb...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Protéines de répression › Protéines du groupe Polycomb › Complexe répresseur Polycomb-2 › Integrated multi-omics reveal polycomb...
questionsmedicales.fr › Structures de l'embryon › Placenta › Trophoblastes › Integrated multi-omics reveal polycomb...