Active RhoA Exerts an Inhibitory Effect on the Homeostasis and Angiogenic Capacity of Human Endothelial Cells.
LIM kinase 2
Rho‐associated protein kinase
endothelial cells
endothelial dysfunction
Journal
Journal of the American Heart Association
ISSN: 2047-9980
Titre abrégé: J Am Heart Assoc
Pays: England
ID NLM: 101580524
Informations de publication
Date de publication:
21 06 2022
21 06 2022
Historique:
pubmed:
15
6
2022
medline:
24
6
2022
entrez:
14
6
2022
Statut:
ppublish
Résumé
Background The small GTPase RhoA (Ras homolog gene family, member A) regulates a variety of cellular processes, including cell motility, proliferation, survival, and permeability. In addition, there are reports indicating that RhoA-ROCK (rho associated coiled-coil containing protein kinase) activation is essential for VEGF (vascular endothelial growth factor)-mediated angiogenesis, whereas other work suggests VEGF-antagonistic effects of the RhoA-ROCK axis. Methods and Results To elucidate this issue, we examined human umbilical vein endothelial cells and human coronary artery endothelial cells after stable overexpression (lentiviral transduction) of constitutively active (G14V/Q63L), dominant-negative (T19N), or wild-type RhoA using a series of in vitro angiogenesis assays (proliferation, migration, tube formation, angiogenic sprouting, endothelial cell viability) and a human umbilical vein endothelial cells xenograft assay in immune-incompetent NOD
Identifiants
pubmed: 35699166
doi: 10.1161/JAHA.121.025119
pmc: PMC9238636
doi:
Substances chimiques
Vascular Endothelial Growth Factor A
0
RHOA protein, human
124671-05-2
LIMK1 protein, human
EC 2.7.11.1
Lim Kinases
EC 2.7.11.1
ROCK1 protein, human
EC 2.7.11.1
rho-Associated Kinases
EC 2.7.11.1
rhoA GTP-Binding Protein
EC 3.6.5.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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