Influence of periodontitis and diabetes on structure and cytokine content of platelet-rich fibrin.
FE-SEM
PRF
diabetes
fiber
growth factors
periodontitis
platelet concentrate
porosity
Journal
Oral diseases
ISSN: 1601-0825
Titre abrégé: Oral Dis
Pays: Denmark
ID NLM: 9508565
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
revised:
19
05
2022
received:
07
02
2022
accepted:
10
06
2022
medline:
7
12
2023
pubmed:
15
6
2022
entrez:
14
6
2022
Statut:
ppublish
Résumé
Platelet-rich fibrin (PRF) is a second-generation platelet concentrate with multiple applications in wound healing and regeneration in both periodontitis and diabetes. However, the three dimensional (3-D) structure and cytokine content of PRF might be altered in patients suffering from either/both of the chronic inflammatory conditions, ultimately influencing the efficacy of PRF as a biomaterial for regenerative medicine. The aim of the present study was hence to evaluate the effect of both these chronic inflammatory diseases on the 3-D structure of PRF membrane. An attempt was also made to compare the growth factor content between the plasma and RBC ends of the prepared PRF gel. L-PRF was prepared for twenty participants, healthy (5), periodontitis (5), T2DM (5) and T2DM with periodontitis (5). Porosity and fiber diameter of PRF membranes was visualized under FE-SEM and measured using ImageJ Software. PDGF-BB and TGF-β1 levels in PRF gel were assessed by ELISA. The average diameter of fibrin fibers under FE-SEM was 0.15 to 0.30 micrometers. Porosity was higher at the plasma end (p = 0.042). Red blood cell (RBC) end of the membrane had thinner fibers arranged in a comparatively more dense and compact structure with smaller porosities. Healthy subjects had the least porous PRF compared to subjects with either/both of the chronic conditions. PDGF-BB levels were similar along all the four groups. TGF-β1 levels were highest in healthy subjects. 3-D structure and growth factor content of PRF are influenced by a person's periodontal and/or diabetic status. The RBC end of the PRF membrane, as compared to the plasma end, has thinner fibers arranged in a comparatively more dense and compact structure with smaller porosities, and hence should be favored during periodontal regenerative procedures. Both periodontitis and diabetes have a significant influence on the 3-D structure and growth factor content of PRF produced.
Sections du résumé
BACKGROUND
BACKGROUND
Platelet-rich fibrin (PRF) is a second-generation platelet concentrate with multiple applications in wound healing and regeneration in both periodontitis and diabetes. However, the three dimensional (3-D) structure and cytokine content of PRF might be altered in patients suffering from either/both of the chronic inflammatory conditions, ultimately influencing the efficacy of PRF as a biomaterial for regenerative medicine.
AIM
OBJECTIVE
The aim of the present study was hence to evaluate the effect of both these chronic inflammatory diseases on the 3-D structure of PRF membrane. An attempt was also made to compare the growth factor content between the plasma and RBC ends of the prepared PRF gel.
MATERIALS & METHODS
METHODS
L-PRF was prepared for twenty participants, healthy (5), periodontitis (5), T2DM (5) and T2DM with periodontitis (5). Porosity and fiber diameter of PRF membranes was visualized under FE-SEM and measured using ImageJ Software. PDGF-BB and TGF-β1 levels in PRF gel were assessed by ELISA.
RESULTS
RESULTS
The average diameter of fibrin fibers under FE-SEM was 0.15 to 0.30 micrometers. Porosity was higher at the plasma end (p = 0.042). Red blood cell (RBC) end of the membrane had thinner fibers arranged in a comparatively more dense and compact structure with smaller porosities. Healthy subjects had the least porous PRF compared to subjects with either/both of the chronic conditions. PDGF-BB levels were similar along all the four groups. TGF-β1 levels were highest in healthy subjects.
DISCUSSION
CONCLUSIONS
3-D structure and growth factor content of PRF are influenced by a person's periodontal and/or diabetic status. The RBC end of the PRF membrane, as compared to the plasma end, has thinner fibers arranged in a comparatively more dense and compact structure with smaller porosities, and hence should be favored during periodontal regenerative procedures.
CONCLUSION
CONCLUSIONS
Both periodontitis and diabetes have a significant influence on the 3-D structure and growth factor content of PRF produced.
Substances chimiques
Cytokines
0
Becaplermin
1B56C968OA
Transforming Growth Factor beta1
0
Intercellular Signaling Peptides and Proteins
0
Types de publication
Journal Article
Langues
eng
Pagination
3620-3629Subventions
Organisme : Department of Science and Technology and Renewable Energy, Chandigarh, India
ID : sanction letter number S&T&RE/RP/147/e-2873/(19-20
Informations de copyright
© 2022 Wiley Periodicals LLC.
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