Duplication of NRAMP3 gene in poplars generated two homologous transporters with distinct functions.
Arabidopsis
Populus
Salix
Trans Golgi Network
apoplasmic transport
evolution
manganese
metal
micronutrient
nutrition
recycling
selective pressure
symplasmic transport
vacuole
Journal
Molecular biology and evolution
ISSN: 1537-1719
Titre abrégé: Mol Biol Evol
Pays: United States
ID NLM: 8501455
Informations de publication
Date de publication:
14 Jun 2022
14 Jun 2022
Historique:
received:
15
12
2021
revised:
28
05
2022
accepted:
02
06
2022
entrez:
14
6
2022
pubmed:
15
6
2022
medline:
15
6
2022
Statut:
aheadofprint
Résumé
Transition metals are essential for a wealth of metabolic reactions, but their concentrations need to be tightly controlled across cells and cell compartments, as metal excess or imbalance has deleterious effects. Metal homeostasis is achieved by a combination of metal transport across membranes and metal binding to a variety of molecules. Gene duplication is a key process in evolution, as emergence of advantageous mutations on one of the copies can confer a new function. Here, we report that the poplar genome contains two paralogues encoding NRAMP3 metal transporters localized in tandem. All Populus species analyzed had two copies of NRAMP3, whereas only one could be identified in Salix species indicating that duplication occurred when the two genera separated. Both copies are under purifying selection and encode functional transporters, as shown by expression in the yeast heterologous expression system. However, genetic complementation revealed that only one of the paralogues has retained the original function in release of metals stored in the vacuole previously characterized in A. thaliana. Confocal imaging showed that the other copy has acquired a distinct localization to the Trans Golgi Network (TGN). Expression in poplar suggested that the copy of NRAMP3 localized on the TGN has a novel function in the control of cell-to-cell transport of manganese. This work provides a clear case of neo-functionalization through change in the subcellular localization of a metal transporter as well as evidence for the involvement of the secretory pathway in cell-to-cell transport of manganese.
Identifiants
pubmed: 35700212
pii: 6608349
doi: 10.1093/molbev/msac129
pmc: PMC9234761
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© The Author(s) 2022. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution.
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