Transcriptional Perturbations of 2,6-Diaminopurine and 2-Aminopurine.
Journal
ACS chemical biology
ISSN: 1554-8937
Titre abrégé: ACS Chem Biol
Pays: United States
ID NLM: 101282906
Informations de publication
Date de publication:
15 07 2022
15 07 2022
Historique:
pubmed:
15
6
2022
medline:
19
7
2022
entrez:
14
6
2022
Statut:
ppublish
Résumé
2,6-Diaminopurine (Z) is a naturally occurring adenine (A) analog that bacteriophages employ in place of A in their genetic alphabet. Recent discoveries of biogenesis pathways of Z in bacteriophages have stimulated substantial research interest in this DNA modification. Here, we systematically examined the effects of Z on the efficiency and fidelity of DNA transcription. Our results showed that Z exhibited no mutagenic yet substantial inhibitory effects on transcription mediated by purified T7 RNA polymerase and by human RNA polymerase II in HeLa nuclear extracts and in human cells. A structurally related adenine analog, 2-aminopurine (2AP), strongly blocked T7 RNA polymerase but did not impede human RNA polymerase II
Identifiants
pubmed: 35700389
doi: 10.1021/acschembio.2c00369
pmc: PMC9354233
mid: NIHMS1827269
doi:
Substances chimiques
2-Aminopurine
452-06-2
2,6-diaminopurine
49P95BAU4Z
DNA
9007-49-2
RNA Polymerase II
EC 2.7.7.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1672-1676Subventions
Organisme : NCI NIH HHS
ID : P01 CA092584
Pays : United States
Organisme : NIEHS NIH HHS
ID : R35 ES031707
Pays : United States
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