Adipose tissue derived stromal cells in a gelatin-based 3D matrix with exclusive ascorbic acid signalling emerged as a novel neural tissue engineering construct: an innovative prototype for soft tissue.
adipose derived stem cells (ASCs)
ascorbic acid
neuronal differentiation
soft tissue engineering
triad
Journal
Regenerative biomaterials
ISSN: 2056-3418
Titre abrégé: Regen Biomater
Pays: England
ID NLM: 101652150
Informations de publication
Date de publication:
2022
2022
Historique:
received:
11
12
2021
revised:
30
03
2022
accepted:
05
05
2022
entrez:
15
6
2022
pubmed:
16
6
2022
medline:
16
6
2022
Statut:
epublish
Résumé
The current study investigated a triad, which comprises of adipose tissue derived stem cells isolated from infrapatellar fat pad and gelatin/polyvinyl alcohol (PVA)-based matrix with exclusive ascorbic acid signalling. Though, the bio-mechanical properties of the gelatin-PVA blended scaffolds in wet condition are equivalent to the ECM of soft tissues in general, in this study, the triad was tested as a model for neural tissue engineering. Apart from being cytocompatible and biocompatible, the porosity of the scaffold has been designed in such a manner that it facilitates the cell signalling and enables the exchange of nutrients and gases. The highly proliferative stem cells from Passage 2 were characterized using both, mesenchymal and embryonic stem cell markers. As an initial exploration the mesenchymal stem cells at Passage 4 were exposed to ascorbic acid and basic fibroblast growth factor signalling for neuronal differentiation in 2D environment independently. The MSCs successfully differentiated and acquired neuron specific markers related to cytoskeleton and synapses. Subsequently, three phases of experiments have been conducted on the 3D gelatin/PVA matrix to prove their efficacy, the growth of stem cells, growth of differentiated neurons and the
Identifiants
pubmed: 35702348
doi: 10.1093/rb/rbac031
pii: rbac031
pmc: PMC9188297
doi:
Types de publication
Journal Article
Langues
eng
Pagination
rbac031Informations de copyright
© The Author(s) 2022. Published by Oxford University Press.
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