Molecular profiling of stem cell-derived retinal pigment epithelial cell differentiation established for clinical translation.
Large-eyed model
age-related macular degeneration
cellular profiling and transcriptome
cellular therapy
clinical translation
differentiation protocol dynamics
human embryonic stem cell-derived retinal pigment epithelial cell
retinal progenitor cells
single-cell RNA sequencing
subretinal injection
Journal
Stem cell reports
ISSN: 2213-6711
Titre abrégé: Stem Cell Reports
Pays: United States
ID NLM: 101611300
Informations de publication
Date de publication:
14 06 2022
14 06 2022
Historique:
received:
27
03
2022
revised:
12
05
2022
accepted:
13
05
2022
entrez:
15
6
2022
pubmed:
16
6
2022
medline:
18
6
2022
Statut:
ppublish
Résumé
Human embryonic stem cell-derived retinal pigment epithelial cells (hESC-RPE) are a promising cell source to treat age-related macular degeneration (AMD). Despite several ongoing clinical studies, a detailed mapping of transient cellular states during in vitro differentiation has not been performed. Here, we conduct single-cell transcriptomic profiling of an hESC-RPE differentiation protocol that has been developed for clinical use. Differentiation progressed through a culture diversification recapitulating early embryonic development, whereby cells rapidly acquired a rostral embryo patterning signature before converging toward the RPE lineage. At intermediate steps, we identified and examined the potency of an NCAM1
Identifiants
pubmed: 35705015
pii: S2213-6711(22)00257-0
doi: 10.1016/j.stemcr.2022.05.005
pmc: PMC9214069
pii:
doi:
Substances chimiques
Retinal Pigments
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1458-1475Subventions
Organisme : NCI NIH HHS
ID : P50 CA211015
Pays : United States
Informations de copyright
Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.
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