Mechanism of replication origin melting nucleated by CMG helicase assembly.
Adenosine Triphosphate
/ metabolism
Cell Cycle Proteins
/ chemistry
Chromatin
Cryoelectron Microscopy
DNA
/ chemistry
DNA Replication
DNA-Binding Proteins
/ chemistry
In Vitro Techniques
Minichromosome Maintenance Proteins
/ chemistry
Nuclear Proteins
Nucleic Acid Denaturation
Protein Conformation
Replication Origin
Saccharomyces cerevisiae Proteins
/ chemistry
Journal
Nature
ISSN: 1476-4687
Titre abrégé: Nature
Pays: England
ID NLM: 0410462
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
received:
20
12
2021
accepted:
03
05
2022
pubmed:
16
6
2022
medline:
2
7
2022
entrez:
15
6
2022
Statut:
ppublish
Résumé
The activation of eukaryotic origins of replication occurs in temporally separated steps to ensure that chromosomes are copied only once per cell cycle. First, the MCM helicase is loaded onto duplex DNA as an inactive double hexamer. Activation occurs after the recruitment of a set of firing factors that assemble two Cdc45-MCM-GINS (CMG) holo-helicases. CMG formation leads to the underwinding of DNA on the path to the establishment of the replication fork, but whether DNA becomes melted at this stage is unknown
Identifiants
pubmed: 35705812
doi: 10.1038/s41586-022-04829-4
pii: 10.1038/s41586-022-04829-4
pmc: PMC9242855
doi:
Substances chimiques
CDC45 protein, S cerevisiae
0
Cell Cycle Proteins
0
Chromatin
0
DNA-Binding Proteins
0
Nuclear Proteins
0
Saccharomyces cerevisiae Proteins
0
Adenosine Triphosphate
8L70Q75FXE
DNA
9007-49-2
MCM2 protein, S cerevisiae
EC 3.6.4.12
Minichromosome Maintenance Proteins
EC 3.6.4.12
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1007-1014Subventions
Organisme : Wellcome Trust
ID : FC001065
Pays : United Kingdom
Organisme : Arthritis Research UK
ID : FC001065
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : European Research Council
ID : 820102
Pays : International
Organisme : Arthritis Research UK
ID : FC001066
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 106252/Z/14/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : FC001066
Pays : United Kingdom
Organisme : European Research Council
ID : 669424-CHROMOREP
Pays : International
Informations de copyright
© 2022. The Author(s).
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