Broad coverage of neutralization-resistant SIV strains by second-generation SIV-specific antibodies targeting the region involved in binding CD4.
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
received:
24
11
2021
accepted:
06
05
2022
revised:
29
06
2022
pubmed:
17
6
2022
medline:
2
7
2022
entrez:
16
6
2022
Statut:
epublish
Résumé
Both SIV and SHIV are powerful tools for evaluating antibody-mediated prevention and treatment of HIV-1. However, owing to a lack of rhesus-derived SIV broadly neutralizing antibodies (bnAbs), testing of bnAbs for HIV-1 prevention or treatment has thus far been performed exclusively in the SHIV NHP model using bnAbs from HIV-1-infected individuals. Here we describe the isolation and characterization of multiple rhesus-derived SIV bnAbs capable of neutralizing most isolates of SIV. Eight antibodies belonging to two clonal families, ITS102 and ITS103, which target unique epitopes in the CD4 binding site (CD4bs) region, were found to be broadly neutralizing and together neutralized all SIV strains tested. A rare feature of these bnAbs and two additional antibody families, ITS92 and ITS101, which mediate strain-specific neutralizing activity against SIV from sooty mangabeys (SIVsm), was their ability to achieve near complete (i.e. 100%) neutralization of moderately and highly neutralization-resistant SIV. Overall, these newly identified SIV bnAbs highlight the potential for evaluating HIV-1 prophylactic and therapeutic interventions using fully simian, rhesus-derived bnAbs in the SIV NHP model, thereby circumventing issues related to rapid antibody clearance of human-derived antibodies, Fc mismatch and limited genetic diversity of SHIV compared to SIV.
Identifiants
pubmed: 35709309
doi: 10.1371/journal.ppat.1010574
pii: PPATHOGENS-D-21-02365
pmc: PMC9242510
doi:
Substances chimiques
Antibodies, Neutralizing
0
Broadly Neutralizing Antibodies
0
HIV Antibodies
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1010574Subventions
Organisme : CCR NIH HHS
ID : HHSN261200800001C
Pays : United States
Organisme : NCI NIH HHS
ID : HHSN261200800001E
Pays : United States
Organisme : NIAID NIH HHS
ID : HHSN272201800004C
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI112456
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Immunity. 2012 Sep 21;37(3):412-25
pubmed: 22999947
PLoS Pathog. 2018 Dec 5;14(12):e1007395
pubmed: 30517201
AIDS Res Hum Retroviruses. 2011 May;27(5):487-500
pubmed: 20854170
PLoS Pathog. 2015 Aug 12;11(8):e1005110
pubmed: 26267277
J Virol. 2016 Nov 14;90(23):10574-10586
pubmed: 27654288
J Virol. 1997 Oct;71(10):7895-902
pubmed: 9311879
J Virol. 1993 Jul;67(7):4104-13
pubmed: 8510218
J Virol. 2013 May;87(10):5477-92
pubmed: 23468494
J Virol. 2017 Sep 27;91(20):
pubmed: 28768869
Cell. 2016 Sep 8;166(6):1471-1484.e18
pubmed: 27610571
Hum Vaccin. 2009 Mar;5(3):141-50
pubmed: 18690014
Science. 2012 Mar 9;335(6073):1188-93
pubmed: 22403383
J Virol. 2007 Aug;81(15):7913-23
pubmed: 17507488
Nucleic Acids Res. 2015 Jan;43(Database issue):D413-22
pubmed: 25378316
Front Immunol. 2016 Jan 28;7:12
pubmed: 26858716
Front Immunol. 2017 Oct 27;8:1407
pubmed: 29163486
Retrovirology. 2018 Oct 16;15(1):70
pubmed: 30326938
J Virol. 2012 Dec;86(24):13217-31
pubmed: 23055550
J Virol. 2009 Jul;83(14):7337-48
pubmed: 19439467
J Virol. 2011 May;85(10):4828-40
pubmed: 21389135
J Infect Dis. 2021 Feb 13;223(3):370-380
pubmed: 32604408
Sci Transl Med. 2019 Oct 30;11(516):
pubmed: 31666399
Sci Transl Med. 2017 Sep 6;9(406):
pubmed: 28878010
J Virol. 2005 Oct;79(19):12455-63
pubmed: 16160173
Nature. 2012 Nov 15;491(7424):406-12
pubmed: 23151583
Front Immunol. 2016 Sep 21;7:372
pubmed: 27708645
Immunity. 2019 Oct 15;51(4):724-734.e4
pubmed: 31586542
AIDS Res Hum Retroviruses. 1990 Nov;6(11):1221-31
pubmed: 2078405
Nat Protoc. 2013 Oct;8(10):1907-15
pubmed: 24030440
Cell Host Microbe. 2014 Sep 10;16(3):304-13
pubmed: 25211073
Science. 2011 Sep 16;333(6049):1593-602
pubmed: 21835983
J Virol. 2016 Dec 16;91(1):
pubmed: 27795431
Cell. 2015 Jun 4;161(6):1280-92
pubmed: 26004070
J Virol. 2011 Jun;85(12):5764-72
pubmed: 21490100
PLoS One. 2021 Mar 22;16(3):e0248973
pubmed: 33752225
Nat Protoc. 2016 Oct;11(10):1908-1923
pubmed: 27658009
AIDS Res Hum Retroviruses. 1992 Mar;8(3):367-72
pubmed: 1571196
AIDS Res Hum Retroviruses. 2017 Aug;33(8):869-879
pubmed: 28075174
Immunity. 2019 Feb 19;50(2):520-532.e3
pubmed: 30709739
PLoS Pathog. 2016 Apr 11;12(4):e1005537
pubmed: 27064278
J Virol. 2014 Nov;88(21):12669-82
pubmed: 25142607
N Engl J Med. 2012 Apr 5;366(14):1275-86
pubmed: 22475592
J Virol. 2010 Feb;84(3):1439-52
pubmed: 19939925
PLoS One. 2015 Mar 25;10(3):e0120451
pubmed: 25807114
AIDS. 2009 Nov 27;23(18):2405-14
pubmed: 19770692
Immunity. 2016 Nov 15;45(5):1108-1121
pubmed: 27851912
Retrovirology. 2018 Sep 5;15(1):61
pubmed: 30185183
PLoS Pathog. 2010 Aug 05;6(8):e1001028
pubmed: 20700449
J Virol. 2015 Aug;89(16):8130-51
pubmed: 26018167
J Virol. 2016 Apr 29;90(10):4966-4980
pubmed: 26937037
PLoS Comput Biol. 2019 Jun 6;15(6):e1007056
pubmed: 31170145
Structure. 2019 Jan 2;27(1):196-206.e6
pubmed: 30471922
Nat Commun. 2016 Dec 20;7:13642
pubmed: 27995928
AIDS. 2014 Jan 14;28(2):163-9
pubmed: 24361678
Immunity. 2018 May 15;48(5):855-871
pubmed: 29768174
J Virol. 2015 Dec 30;90(6):2740-55
pubmed: 26719262
J Virol. 2011 Oct;85(19):10389-98
pubmed: 21775457
Immunity. 2013 Aug 22;39(2):245-58
pubmed: 23911655
Nature. 2016 May 5;533(7601):105-109
pubmed: 27120156
Science. 2010 Aug 13;329(5993):811-7
pubmed: 20616231
J Virol. 2009 Mar;83(6):2686-96
pubmed: 19129440
Cell. 2019 Jul 25;178(3):567-584.e19
pubmed: 31348886
Nature. 2011 Sep 22;477(7365):466-70
pubmed: 21849977
J Virol. 2010 Feb;84(3):1631-6
pubmed: 19923174
Nat Immunol. 2018 Nov;19(11):1179-1188
pubmed: 30333615
Sci Transl Med. 2017 Sep 20;9(408):
pubmed: 28931655
Science. 2010 Aug 13;329(5993):856-61
pubmed: 20616233
Immunol Rev. 2013 Jul;254(1):225-44
pubmed: 23772623
Curr Protoc Immunol. 2005 Jan;Chapter 12:Unit 12.11
pubmed: 18432938
J Struct Biol. 2012 Dec;180(3):519-30
pubmed: 23000701