The cellular architecture and molecular determinants of the zebrafish fusogenic synapse.
Jam2a/Jamb
Jam3b/Jamc
Myomaker
Myomixer
WASP and WAVE
actin cytoskeleton
cell-cell fusion
invasive membrane protrusions
myoblast fusion
zebrafish muscle development
Journal
Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028
Informations de publication
Date de publication:
11 07 2022
11 07 2022
Historique:
received:
08
09
2021
revised:
04
04
2022
accepted:
20
05
2022
pubmed:
17
6
2022
medline:
15
7
2022
entrez:
16
6
2022
Statut:
ppublish
Résumé
Myoblast fusion is an indispensable process in skeletal muscle development and regeneration. Studies in Drosophila led to the discovery of the asymmetric fusogenic synapse, in which one cell invades its fusion partner with actin-propelled membrane protrusions to promote fusion. However, the timing and sites of vertebrate myoblast fusion remain elusive. Here, we show that fusion between zebrafish fast muscle cells is mediated by an F-actin-enriched invasive structure. Two cell adhesion molecules, Jam2a and Jam3b, are associated with the actin structure, with Jam2a being the major organizer. The Arp2/3 actin nucleation-promoting factors, WAVE and WASP-but not the bipartite fusogenic proteins, Myomaker or Myomixer-promote the formation of the invasive structure. Moreover, the convergence of fusogen-containing microdomains and the invasive protrusions is a prerequisite for cell membrane fusion. Thus, our study provides unprecedented insights into the cellular architecture and molecular determinants of the asymmetric fusogenic synapse in an intact vertebrate animal.
Identifiants
pubmed: 35709765
pii: S1534-5807(22)00373-2
doi: 10.1016/j.devcel.2022.05.016
pmc: PMC10180866
mid: NIHMS1811041
pii:
doi:
Substances chimiques
Actins
0
Membrane Proteins
0
Muscle Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1582-1597.e6Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR053173
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR075005
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM136316
Pays : United States
Organisme : NIH HHS
ID : S10 OD021685
Pays : United States
Informations de copyright
Copyright © 2022 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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