Design of a multi-epitope vaccine candidate against Brucella melitensis.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
16 06 2022
16 06 2022
Historique:
received:
24
01
2022
accepted:
07
06
2022
entrez:
17
6
2022
pubmed:
18
6
2022
medline:
22
6
2022
Statut:
epublish
Résumé
Brucella is a typical facultative intracellular bacterium that can cause zoonotic infections. For Brucella, it is difficult to eliminate with current medical treatment. Therefore, a multi-epitope vaccine (MEV) should be designed to prevent Brucella infection. For this purpose, we applied the reverse vaccinology approach from Omp10, Omp25, Omp31 and BtpB. Finally, we obtained 13 cytotoxic T lymphocyte (CTL) epitopes, 17 helper T lymphocyte (HTL) epitopes, 9 linear B cell epitopes, and 2 conformational B cell epitopes for further study. To keep the protein folded normally, we linked AAY, GPGPG, and KK to CTL epitopes, HTL epitopes, and B cell epitopes, respectively. The N-terminal of the vaccine peptide is supplemented with appropriate adjuvants to enhance immunogenicity. To evaluate its immunogenicity, stability, safety, and feasibility, a final MEV containing 806 amino acids was constructed by linking linkers and adjuvants. In addition, molecular docking and molecular dynamics simulations were performed to verify the affinity and stability of the MEV-TLR4. Then, codon adaptation and in silico cloning studies were carried out to identify the possible codons for expressing the MEV. In animal experiments, the results demonstrated that the MEV had high immunogenicity. Collectively, this study provided a theoretical basis for the development of a Brucella vaccine.
Identifiants
pubmed: 35710873
doi: 10.1038/s41598-022-14427-z
pii: 10.1038/s41598-022-14427-z
pmc: PMC9202987
doi:
Substances chimiques
Epitopes, B-Lymphocyte
0
Epitopes, T-Lymphocyte
0
Vaccines, Subunit
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
10146Informations de copyright
© 2022. The Author(s).
Références
Enzyme Microb Technol. 2020 Oct;140:109647
pubmed: 32912698
PLoS One. 2010 Nov 04;5(11):e13458
pubmed: 21079793
Sci Rep. 2021 Sep 2;11(1):17626
pubmed: 34475453
Vet Res. 2021 Jun 2;52(1):75
pubmed: 34078437
Front Immunol. 2020 Jul 28;11:1784
pubmed: 32849643
J Med Case Rep. 2007 Jul 21;1:53
pubmed: 17659088
Infect Dis Poverty. 2020 May 24;9(1):55
pubmed: 32448394
Front Cell Infect Microbiol. 2013 Jul 08;3:28
pubmed: 23847770
Front Immunol. 2022 Feb 18;13:806825
pubmed: 35250977
J Comput Chem. 2005 Dec;26(16):1701-18
pubmed: 16211538
Front Immunol. 2021 Aug 12;12:668492
pubmed: 34456902
Am J Pathol. 2015 Jun;185(6):1505-17
pubmed: 25892682
Mol Immunol. 2017 Nov;91:65-74
pubmed: 28886588
Int J Pept Res Ther. 2022;28(2):50
pubmed: 35069056
Int J Mol Sci. 2021 Nov 26;22(23):
pubmed: 34884620
Front Immunol. 2020 Apr 07;11:442
pubmed: 32318055
J Proteome Res. 2020 Jun 5;19(6):2304-2315
pubmed: 32308001
BMC Bioinformatics. 2013;14 Suppl 4:S2
pubmed: 23514126
Biochem Biophys Res Commun. 2021 Feb 12;540:37-41
pubmed: 33429198
Chem Sci. 2016 Feb 1;7(2):842-854
pubmed: 28791117
Science. 2021 Aug 20;373(6557):871-876
pubmed: 34282049
J Immunol Res. 2017;2017:2680160
pubmed: 29445754
Netw Model Anal Health Inform Bioinform. 2021;10(1):37
pubmed: 34094807
J Immunol Methods. 2022 Jan;500:113172
pubmed: 34673003
FASEB J. 2021 Mar;35(3):e21409
pubmed: 33577115
J Chem Theory Comput. 2015 Aug 11;11(8):3696-713
pubmed: 26574453
Vaccines (Basel). 2021 Sep 26;9(10):
pubmed: 34696187
Int J Pept Res Ther. 2021;27(2):1469-1480
pubmed: 33679273
Hum Vaccin Immunother. 2021 Nov 2;17(11):3855-3870
pubmed: 34613880
Sci Rep. 2019 Feb 21;9(1):2517
pubmed: 30792446
Immune Netw. 2015 Apr;15(2):51-7
pubmed: 25922593
Nucleic Acids Res. 2020 Jul 2;48(W1):W449-W454
pubmed: 32406916
Nucleic Acids Res. 2017 Jul 3;45(W1):W365-W373
pubmed: 28521030
Biosci Rep. 2019 Jun 28;39(6):
pubmed: 31196965
Methods Mol Biol. 2009;524:427-38
pubmed: 19377963
Comput Biol Med. 2016 Sep 1;76:24-9
pubmed: 27393958
Jpn J Infect Dis. 2018 May 24;71(3):197-204
pubmed: 29709972
Front Vet Sci. 2017 Dec 13;4:197
pubmed: 29326948
Cell Mol Immunol. 2018 Feb;15(2):182-184
pubmed: 28890542
Iran J Microbiol. 2013 Mar;5(1):19-23
pubmed: 23467315
Sci Rep. 2018 Jun 13;8(1):9044
pubmed: 29899345
Sci Rep. 2019 Mar 5;9(1):3512
pubmed: 30837598
Brain Behav Immun. 2021 Jan;91:296-314
pubmed: 33039659
Microbes Infect. 2012 Jul;14(7-8):639-50
pubmed: 22387699
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W407-10
pubmed: 17517781
PLoS One. 2013 Sep 13;8(9):e73957
pubmed: 24058508
Vaccines (Basel). 2021 Sep 23;9(10):
pubmed: 34696166
Bioinformatics. 2009 Sep 1;25(17):2200-7
pubmed: 19549632
Microb Pathog. 2021 Oct;159:105150
pubmed: 34425197
Vet World. 2018 Aug;11(8):1030-1036
pubmed: 30250359
Bioinformatics. 2009 May 1;25(9):1189-91
pubmed: 19151095
BMC Bioinformatics. 2010 Aug 18;11:431
pubmed: 20718988
Sci Rep. 2020 Apr 14;10(1):6368
pubmed: 32286356
Biotechnol Adv. 2012 Sep-Oct;30(5):1102-7
pubmed: 21968145
PLoS One. 2013;8(1):e52773
pubmed: 23382822
J Genet Eng Biotechnol. 2021 May 11;19(1):69
pubmed: 33974183
J Chem Inf Model. 2011 Oct 24;51(10):2778-86
pubmed: 21919503
Vaccine. 2019 Jul 9;37(30):3981-3988
pubmed: 31176541
Protein Sci. 2003 Aug;12(8):1652-62
pubmed: 12876315
Biomed Res Int. 2018 Apr 22;2018:5712920
pubmed: 29850535
Sci Rep. 2018 Aug 14;8(1):12110
pubmed: 30108316
Immunogenetics. 2010 Jun;62(6):357-68
pubmed: 20379710
Epidemiol Infect. 2021 May 25;149:e136
pubmed: 34032200
PLoS One. 2010 Apr 16;5(4):e9862
pubmed: 20419125
Vaccine. 2015 Oct 13;33(42):5532-5538
pubmed: 26384448