The Effect of Aerobic Exercise on the Oxidative Capacity of Skeletal Muscle Mitochondria in Mice with Impaired Glucose Tolerance.
Journal
Journal of diabetes research
ISSN: 2314-6753
Titre abrégé: J Diabetes Res
Pays: England
ID NLM: 101605237
Informations de publication
Date de publication:
2022
2022
Historique:
received:
11
12
2021
revised:
05
05
2022
accepted:
23
05
2022
entrez:
17
6
2022
pubmed:
18
6
2022
medline:
22
6
2022
Statut:
epublish
Résumé
Male C57BL/6J mice were randomly divided into six different experimental groups (8 animals/group): (1) normal group (NOR), (2) normal control group (NC), (3) normal + exercise group (NE), (4) IGT group (IGT), (5) IGT control group (IC), and (6) IGT+ exercise group (IE).The exercise group received aerobic exercise for 8 weeks. After the intervention, a blood glucose meter was used to detect the level of glucose tolerance in the mouse's abdominal cavity; a biochemical kit was used to detect serum lipid metabolism indicators, malondialdehyde, and superoxide dismutase levels; the ELISA method was used to detect serum insulin and mouse gastrocnemius homogenate LDH, PDH, SDH, and CCO levels. Western blot method was used to detect the protein expression levels of NOX4, PGC-1 (1) Mice with high-fat diet for 30 weeks showed impaired glucose tolerance, insulin resistance, and lipid metabolism disorders. The level of LDH, PDH, SDH, and CCO in the gastrocnemius homogenate of mice was reduced. The expressions of NOX4 protein were significantly upregulated, while the expressions of PGC-1 (1) C57BL/6 mice were fed high fat for 30 weeks and successfully constructed a mouse model of reduced diabetes; the mice with reduced diabetes have impaired glucose tolerance, insulin resistance, and lipid metabolism disorders; (2) 8 weeks of aerobic exercise improve glucose tolerance, reduce glucose tolerance in mice, reduce insulin resistance, improve lipid metabolism disorders, and reduce oxidative stress; (3) 8-week aerobic exercise reduces skeletal muscle NOX4 expression and increases glucose tolerance; reduces the expression of LDH, PDH, SDH, and CCO in mouse skeletal muscle; increases the expression level of mitochondrial fusion protein 2 and PGC-1
Identifiants
pubmed: 35712028
doi: 10.1155/2022/3780156
pmc: PMC9197611
doi:
Substances chimiques
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3780156Informations de copyright
Copyright © 2022 Dan Wang et al.
Déclaration de conflit d'intérêts
The authors declared no conflict of interests regarding the publication of this manuscript.
Références
Trends Endocrinol Metab. 2012 Mar;23(3):142-53
pubmed: 22305519
Front Endocrinol (Lausanne). 2019 Feb 28;10:119
pubmed: 30873123
Nat Commun. 2022 Jan 20;13(1):424
pubmed: 35058456
Biol Res. 2020 Sep 14;53(1):39
pubmed: 32928312
Cell Metab. 2013 Feb 5;17(2):162-84
pubmed: 23395166
Diabetes. 2013 Apr;62(4):1036-40
pubmed: 23520283
BMJ Open Diabetes Res Care. 2022 Mar;10(2):
pubmed: 35365489
Diabetes Care. 2007 Mar;30(3):753-9
pubmed: 17327355
Front Med. 2020 Oct;14(5):583-600
pubmed: 32248333
Am J Physiol Endocrinol Metab. 2010 Aug;299(2):E145-61
pubmed: 20371735
J Bodyw Mov Ther. 2011 Jul;15(3):355-62
pubmed: 21665113
Diabetes. 2008 Nov;57(11):2933-42
pubmed: 18716044
Nature. 2008 Dec 4;456(7222):605-10
pubmed: 19052620
Redox Biol. 2017 Apr;11:637-645
pubmed: 28131082
Redox Biol. 2015;4:6-13
pubmed: 25479550
FASEB J. 2002 Dec;16(14):1879-86
pubmed: 12468452
Trends Biochem Sci. 2001 Jan;26(1):23-9
pubmed: 11165513
Diabetes Care. 2006 Jun;29(6):1433-8
pubmed: 16732040
Diabetes Care. 2010 Dec;33(12):2692-6
pubmed: 21115771
PLoS One. 2014 Mar 21;9(3):e92810
pubmed: 24658162
Korean J Physiol Pharmacol. 2017 Nov;21(6):567-577
pubmed: 29200899
Diabetes Care. 2006 May;29(5):1130-9
pubmed: 16644654
J Diabetes Res. 2020 Jan 21;2020:2521590
pubmed: 32051831
Crit Rev Food Sci Nutr. 2014;54(12):1599-616
pubmed: 24580561
Ann Intern Med. 2007 Sep 18;147(6):357-69
pubmed: 17876019
Cell Metab. 2020 Apr 7;31(4):791-808.e8
pubmed: 32220306
J Endocrinol. 2017 Apr;233(1):R15-R42
pubmed: 28232636
Diabetes Care. 2009 Nov;32 Suppl 2:S157-63
pubmed: 19875544
Int J Mol Sci. 2016 Oct 22;17(10):
pubmed: 27782077
Cell Metab. 2013 Apr 2;17(4):491-506
pubmed: 23562075
Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5523-8
pubmed: 22427360
Nature. 2001 Sep 13;413(6852):131-8
pubmed: 11557972
Cell Res. 2018 Oct;28(10):969-980
pubmed: 30108290
Nature. 2002 Aug 15;418(6899):797-801
pubmed: 12181572
Cell Metab. 2008 Jan;7(1):45-56
pubmed: 18177724
Trends Endocrinol Metab. 2012 Sep;23(9):459-66
pubmed: 22817841
JAMA. 2011 May 4;305(17):1790-9
pubmed: 21540423
J Clin Periodontol. 2017 Jun;44(6):568-576
pubmed: 28419499
J Appl Physiol (1985). 2011 Jan;110(1):264-74
pubmed: 21030673
Endocr Rev. 2010 Jun;31(3):364-95
pubmed: 20156986
Redox Biol. 2014;2:170-7
pubmed: 25460725
Transl Res. 2018 Dec;202:69-82
pubmed: 30153426
Lancet. 2014 Aug 30;384(9945):766-81
pubmed: 24880830
Biochim Biophys Acta. 2010 Jun-Jul;1797(6-7):1028-33
pubmed: 20175989
Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7627-8
pubmed: 18509057
Gastroenterology. 2015 Aug;149(2):468-80.e10
pubmed: 25888330
Diabetes. 2005 Jan;54(1):8-14
pubmed: 15616005
Diabetes. 2006 Jun;55(6):1783-91
pubmed: 16731843
Free Radic Biol Med. 2016 Sep;98:18-28
pubmed: 27184955
Circulation. 2013 May 7;127(18):1888-902
pubmed: 23564668
Pflugers Arch. 2010 Sep;460(4):703-18
pubmed: 20652307
Hum Mol Genet. 2005 Jun 1;14(11):1405-15
pubmed: 15829499
Nutr Metab (Lond). 2010 Apr 30;7:36
pubmed: 20433743
Science. 2013 Apr 26;340(6131):471-5
pubmed: 23620051
Diabetes. 2012 Feb;61(2):447-53
pubmed: 22275086
Diabetes. 1988 Sep;37(9):1163-7
pubmed: 3044882
J Biol Chem. 2003 May 9;278(19):17190-7
pubmed: 12598526
Sci Rep. 2016 Sep 29;6:33944
pubmed: 27681159
Curr Diab Rep. 2002 Jun;2(3):242-8
pubmed: 12643180
Annu Rev Pathol. 2020 Jan 24;15:235-259
pubmed: 31585519
Cell Metab. 2012 May 2;15(5):595-605
pubmed: 22560212