Rapid removal of detergent in glycolipids using ionic liquids.
1,2-dichloroethane
Ganglioside
Glycosphingolipid
Ionic liquid
Thin-layer chromatography
Journal
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
ISSN: 1348-2246
Titre abrégé: Anal Sci
Pays: Switzerland
ID NLM: 8511078
Informations de publication
Date de publication:
Aug 2022
Aug 2022
Historique:
received:
25
02
2022
accepted:
24
05
2022
pubmed:
20
6
2022
medline:
27
7
2022
entrez:
19
6
2022
Statut:
ppublish
Résumé
Detergent removal in glycolipid after sample preparation, such as enzymatic reaction or isolation of detergent-resistant membrane microdomain, is indispensable for further structural characterization. We previously established the rapid and effective method of detergent removal in glycolipid samples from glass test tube using 1,2-dichloroethane (DCE) washing. However, the use of DCE has several drawbacks, such as environmental risks, harmful effects (potentially carcinogenic), and high vaporability and flammability. To solve the issue, we used ionic liquids to remove detergents from glycolipid samples, and found 1-butyl-3-methylimidazolium iodide was a suitable alternative for DCE.
Identifiants
pubmed: 35718829
doi: 10.1007/s44211-022-00139-x
pii: 10.1007/s44211-022-00139-x
doi:
Substances chimiques
Detergents
0
Glycolipids
0
Iodides
0
Ionic Liquids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1115-1121Subventions
Organisme : Grant-in-Aid Scientific Research (A)
ID : 21H04820
Informations de copyright
© 2022. The Author(s), under exclusive licence to The Japan Society for Analytical Chemistry.
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