Rapid removal of detergent in glycolipids using ionic liquids.

1,2-dichloroethane Ganglioside Glycosphingolipid Ionic liquid Thin-layer chromatography

Journal

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
ISSN: 1348-2246
Titre abrégé: Anal Sci
Pays: Switzerland
ID NLM: 8511078

Informations de publication

Date de publication:
Aug 2022
Historique:
received: 25 02 2022
accepted: 24 05 2022
pubmed: 20 6 2022
medline: 27 7 2022
entrez: 19 6 2022
Statut: ppublish

Résumé

Detergent removal in glycolipid after sample preparation, such as enzymatic reaction or isolation of detergent-resistant membrane microdomain, is indispensable for further structural characterization. We previously established the rapid and effective method of detergent removal in glycolipid samples from glass test tube using 1,2-dichloroethane (DCE) washing. However, the use of DCE has several drawbacks, such as environmental risks, harmful effects (potentially carcinogenic), and high vaporability and flammability. To solve the issue, we used ionic liquids to remove detergents from glycolipid samples, and found 1-butyl-3-methylimidazolium iodide was a suitable alternative for DCE.

Identifiants

pubmed: 35718829
doi: 10.1007/s44211-022-00139-x
pii: 10.1007/s44211-022-00139-x
doi:

Substances chimiques

Detergents 0
Glycolipids 0
Iodides 0
Ionic Liquids 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1115-1121

Subventions

Organisme : Grant-in-Aid Scientific Research (A)
ID : 21H04820

Informations de copyright

© 2022. The Author(s), under exclusive licence to The Japan Society for Analytical Chemistry.

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Auteurs

Shoko Matsushita (S)

Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Building No.2, 1-5-1 Kanda Surugadai, Chiyoda, Tokyo, 101-0062, Japan.

Kazuhiko Noda (K)

Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Building No.2, 1-5-1 Kanda Surugadai, Chiyoda, Tokyo, 101-0062, Japan.
ORTHOMEDICO Inc., 2F Sumitomo Fudosan Korakuen Bldg., 1-4-1 Koishikawa, Bunkyo-ku, Tokyo, 112-0002, Japan.

Shuta Hara (S)

Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Building No.2, 1-5-1 Kanda Surugadai, Chiyoda, Tokyo, 101-0062, Japan.
Department of Materials and Life Chemistry, Faculty of Engineering, Kanagawa University, 3-6-1 Kanagawa-ku, Yokohama, 221-8686, Japan.

Jumpei Aisu (J)

Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Building No.2, 1-5-1 Kanda Surugadai, Chiyoda, Tokyo, 101-0062, Japan.

Aki Hayashi (A)

Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Building No.2, 1-5-1 Kanda Surugadai, Chiyoda, Tokyo, 101-0062, Japan.

Hiroki Ikake (H)

Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Building No.2, 1-5-1 Kanda Surugadai, Chiyoda, Tokyo, 101-0062, Japan.

Shigeru Shimizu (S)

Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Building No.2, 1-5-1 Kanda Surugadai, Chiyoda, Tokyo, 101-0062, Japan.

Minoru Tanigawa (M)

Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Building No.2, 1-5-1 Kanda Surugadai, Chiyoda, Tokyo, 101-0062, Japan.

Motohiko Ukiya (M)

Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Building No.2, 1-5-1 Kanda Surugadai, Chiyoda, Tokyo, 101-0062, Japan.

Katsushi Nishimura (K)

Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Building No.2, 1-5-1 Kanda Surugadai, Chiyoda, Tokyo, 101-0062, Japan.

Atsuyoshi Nishina (A)

Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Building No.2, 1-5-1 Kanda Surugadai, Chiyoda, Tokyo, 101-0062, Japan.

Yusuke Suzuki (Y)

Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Building No.2, 1-5-1 Kanda Surugadai, Chiyoda, Tokyo, 101-0062, Japan. suzuki.yuusuke@nihon-u.ac.jp.

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