Identification and Isolation of Type II NKT Cell Subsets in Human Blood and Liver.
FoxP3
NKT
expansion
isolation
liver
steatotic
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2022
2022
Historique:
received:
17
03
2022
accepted:
09
05
2022
entrez:
20
6
2022
pubmed:
21
6
2022
medline:
22
6
2022
Statut:
epublish
Résumé
Steatotic livers are more prone to rejection, but are often transplanted owing to the shortage of available organs. Type II NKT (T2NKT) cells are liver-resident lymphocytes that react to lipids presented by CD1d. The role of T2NKT cells in rejection of fatty liver transplants is unclear, partly because of a lack of T2NKT cell markers and their very low frequency in blood. Here, we quantify human T2NKT cells in blood and liver tissue by flow cytometry and provide a strategy for their enrichment and expansion. Human T2NKT cells were identified as CD3 The frequency of human T2NKT cells in blood was very low (0.8 ± 0.4% of CD3 Our work defines new strategies for identifying and isolating T2NKT cells from human blood and liver tissue. We showed that this rare population can be expanded
Sections du résumé
Background
Steatotic livers are more prone to rejection, but are often transplanted owing to the shortage of available organs. Type II NKT (T2NKT) cells are liver-resident lymphocytes that react to lipids presented by CD1d. The role of T2NKT cells in rejection of fatty liver transplants is unclear, partly because of a lack of T2NKT cell markers and their very low frequency in blood. Here, we quantify human T2NKT cells in blood and liver tissue by flow cytometry and provide a strategy for their enrichment and expansion.
Methods
Human T2NKT cells were identified as CD3
Results
The frequency of human T2NKT cells in blood was very low (0.8 ± 0.4% of CD3
Conclusions
Our work defines new strategies for identifying and isolating T2NKT cells from human blood and liver tissue. We showed that this rare population can be expanded
Identifiants
pubmed: 35720369
doi: 10.3389/fimmu.2022.898473
pmc: PMC9202826
doi:
Substances chimiques
Forkhead Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
898473Informations de copyright
Copyright © 2022 Yang Zhou, Werner, Glehr, Geissler, Hutchinson and Kronenberg.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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