Structural Characterization of a Neutralizing Nanobody With Broad Activity Against SARS-CoV-2 Variants.

COVID-19 SARS-CoV-2 conformation competition coronavirus crystal structure nanobody receptor-binding domain

Journal

Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977

Informations de publication

Date de publication:
2022
Historique:
received: 14 02 2022
accepted: 19 04 2022
entrez: 20 6 2022
pubmed: 21 6 2022
medline: 21 6 2022
Statut: epublish

Résumé

SARS-CoV-2 and its variants, such as the Omicron continue to threaten public health. The virus recognizes the host cell by attaching its Spike (S) receptor-binding domain (RBD) to the host receptor, ACE2. Therefore, RBD is a primary target for neutralizing antibodies and vaccines. Here, we report the isolation and biological and structural characterization of a single-chain antibody (nanobody) from RBD-immunized alpaca. The nanobody, named DL28, binds to RBD tightly with a

Identifiants

DOI: 10.3389/fmicb.2022.875840 PMID: 35722331 PMC: PMC9201380
pubmed: 35722331
doi: 10.3389/fmicb.2022.875840
pmc: PMC9201380
doi:

Types de publication

Journal Article

Langues

eng

Pagination

875840

Informations de copyright

Copyright © 2022 Li, Zhou, Luo, Lai, Huang, Zhou, Li, Gautam, Bourgeau, Wang, Bao, Tan, Lavillette and Li.

Déclaration de conflit d'intérêts

A patent application for potential nanobody therapy for the treatment of COVID-19 has been filed for DL28. YZ, SW, and JT were employed by the company Nanjing Crycision Biotech Co., Ltd., Nanjing, China. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Tingting Li (T)

State Key Laboratory of Molecular Biology, Chinese Academy of Sciences Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.

Bingjie Zhou (B)

University of CAS, Beijing, China.
CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai CAS, Shanghai, China.

Zhipu Luo (Z)

Institute of Molecular Enzymology, School of Biology and Basic Medical Sciences, Soochow University, Suzhou, China.

Yanling Lai (Y)

State Key Laboratory of Molecular Biology, Chinese Academy of Sciences Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.
University of CAS, Beijing, China.

Suqiong Huang (S)

University of CAS, Beijing, China.
CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai CAS, Shanghai, China.
College of Pharmacy, Chongqing Medical University, Chongqing, China.

Yuanze Zhou (Y)

Nanjing Crycision Biotech Co., Ltd., Nanjing, China.

Yaning Li (Y)

State Key Laboratory of Molecular Biology, Chinese Academy of Sciences Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.
University of CAS, Beijing, China.

Anupriya Gautam (A)

University of CAS, Beijing, China.
CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai CAS, Shanghai, China.

Salome Bourgeau (S)

University of CAS, Beijing, China.
CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai CAS, Shanghai, China.
Institut National de la Santé et de la Recherche Médicale, École des Hautes Etudes en Santé Publique, Institut de Recherche en Santé, Environnement et Travail, Université de Rennes, Rennes, France.

Shurui Wang (S)

Nanjing Crycision Biotech Co., Ltd., Nanjing, China.

Juan Bao (J)

State Key Laboratory of Molecular Biology, Chinese Academy of Sciences Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.

Jingquan Tan (J)

Nanjing Crycision Biotech Co., Ltd., Nanjing, China.

Dimitri Lavillette (D)

CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai CAS, Shanghai, China.
Pasteurien College, Soochow University, Suzhou, China.

Dianfan Li (D)

State Key Laboratory of Molecular Biology, Chinese Academy of Sciences Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.

Classifications MeSH