Hypoxia induced ALKBH5 prevents spontaneous abortion by mediating m
ALKBH5
Hypoxia
SMAD1
SMAD5
Trophoblast
m(6)A modification
Journal
Biochimica et biophysica acta. Molecular cell research
ISSN: 1879-2596
Titre abrégé: Biochim Biophys Acta Mol Cell Res
Pays: Netherlands
ID NLM: 101731731
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
received:
30
03
2022
revised:
07
06
2022
accepted:
10
06
2022
pubmed:
21
6
2022
medline:
10
8
2022
entrez:
20
6
2022
Statut:
ppublish
Résumé
The molecules induced by hypoxia have been supposed to be important regulators of first trimester trophoblast activity, but the key mechanism mediating invasion of trophoblast cells is not fully illustrated. Here, we found that the expression of RNA demethylase ALKBH5 was upregulated in trophoblast upon hypoxia treatment and decreased in extravillous trophoblast (EVT) of patients with recurrent spontaneous abortion (RSA). Furthermore, we found that trophoblast-specific knockdown of ALKBH5 in mouse placenta suppressed the invasion of trophoblast and significantly led to fetus abortion in vivo. Then ALKBH5 was identified to promote the invasion of trophoblast. Mechanistically, we identified transcripts with altered methylation in trophoblast induced by hypoxia via m
Identifiants
pubmed: 35724807
pii: S0167-4889(22)00108-2
doi: 10.1016/j.bbamcr.2022.119316
pii:
doi:
Substances chimiques
RNA, Messenger
0
Smad1 Protein
0
Smad1 protein, mouse
0
Smad5 Protein
0
Smad5 protein, mouse
0
ALKBH5 protein, human
EC 1.14.11.-
ALKBH5 protein, mouse
EC 1.14.11.-
AlkB Homolog 5, RNA Demethylase
EC 1.14.11.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
119316Informations de copyright
Copyright © 2022. Published by Elsevier B.V.