Profiling gut microbiota and bile acid metabolism in critically ill children.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
21 06 2022
21 06 2022
Historique:
received:
24
11
2021
accepted:
26
05
2022
entrez:
21
6
2022
pubmed:
22
6
2022
medline:
24
6
2022
Statut:
epublish
Résumé
Broad-spectrum antimicrobial use during the treatment of critical illness influences gastrointestinal fermentation endpoints, host immune response and metabolic activity including the conversion of primary to secondary bile acids. We previously observed reduced fermentation capacity in the faecal microbiota of critically ill children upon hospital admission. Here, we further explore the timecourse of the relationship between the microbiome and bile acid profile in faecal samples collected from critically ill children. The microbiome was assayed by sequencing of the 16S rRNA gene, and faecal water bile acids were measured by liquid chromatography mass spectrometry. In comparison to admission faecal samples, members of the Lachnospiraceae recovered during the late-acute phase (days 8-10) of hospitalisation. Patients with infections had a lower proportion of Lachnospiraceae in their gut microbiota than controls and patients with primary admitting diagnoses. Keystone species linked to ecological recovery were observed to decline with the length of PICU admission. These species were further suppressed in patients with systemic infection, respiratory failure, and undergoing surgery. Bile acid composition recovers quickly after intervention for critical illness which may be aided by the compositional shift in Lachnospiraceae. Our findings suggest gut microbiota recovery can be readily assessed via measurement of faecal bile acids.
Identifiants
pubmed: 35729169
doi: 10.1038/s41598-022-13640-0
pii: 10.1038/s41598-022-13640-0
pmc: PMC9213539
doi:
Substances chimiques
Bile Acids and Salts
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
10432Subventions
Organisme : Wellcome Trust
ID : 215515
Pays : United Kingdom
Organisme : Department of Health
ID : BRC-1215-20014
Pays : United Kingdom
Informations de copyright
© 2022. The Author(s).
Références
Appl Environ Microbiol. 1996 Feb;62(2):656-61
pubmed: 8593067
Nat Ecol Evol. 2020 Sep;4(9):1256-1267
pubmed: 32632261
Cancer Res. 1977 Sep;37(9):3238-42
pubmed: 884672
Appl Environ Microbiol. 2009 Dec;75(23):7537-41
pubmed: 19801464
Microbiol Mol Biol Rev. 2017 Nov 8;81(4):
pubmed: 29118049
Clin Perinatol. 2011 Jun;38(2):321-31
pubmed: 21645799
J Pediatr Gastroenterol Nutr. 2007 Feb;44(2):252-7
pubmed: 17255840
Nat Microbiol. 2020 Sep;5(9):1067-1068
pubmed: 32839573
Front Immunol. 2019 Oct 23;10:2494
pubmed: 31749800
Nutrients. 2019 Dec 07;11(12):
pubmed: 31817895
J Natl Cancer Inst. 1976 Feb;56(2):441-2
pubmed: 1255778
Nat Med. 2016 Mar;22(3):250-3
pubmed: 26828196
Mol Cancer Res. 2007 May;5(5):455-9
pubmed: 17475672
Appl Environ Microbiol. 2006 Mar;72(3):1729-38
pubmed: 16517616
Lancet Respir Med. 2016 Jan;4(1):59-72
pubmed: 26700442
Antonie Van Leeuwenhoek. 2018 Nov;111(11):2107-2128
pubmed: 29855844
Best Pract Res Clin Gastroenterol. 2014 Aug;28(4):573-83
pubmed: 25194176
Appl Microbiol Biotechnol. 2017 Jan;101(1):47-64
pubmed: 27888332
Nature. 2012 Jul 5;487(7405):104-8
pubmed: 22722865
PLoS One. 2018 May 8;13(5):e0196977
pubmed: 29738579
Gut Microbes. 2019;10(4):481-503
pubmed: 30589376
J Lipid Res. 2006 Feb;47(2):241-59
pubmed: 16299351
Clin Biochem. 2020 Aug;82:2-11
pubmed: 32188572
Immunol Rev. 2017 Sep;279(1):23-35
pubmed: 28856736
Microorganisms. 2019 Jan 10;7(1):
pubmed: 30634578
Anal Chem. 2015 Oct 6;87(19):9662-70
pubmed: 26327313
Int J Syst Evol Microbiol. 2018 Dec;68(12):3741-3746
pubmed: 30351260
Am J Clin Pathol. 2011 Oct;136(4):609-16
pubmed: 21917684
Microbiome. 2016 Dec 29;4(1):66
pubmed: 28034303
Cell. 2018 Sep 6;174(6):1406-1423.e16
pubmed: 30193113
Crit Care Med. 2019 Sep;47(9):e727-e734
pubmed: 31169619
Nature. 2018 Oct;562(7728):583-588
pubmed: 30356187
Appl Environ Microbiol. 1981 Mar;41(3):737-45
pubmed: 7224633
Comput Struct Biotechnol J. 2019 Jul 26;17:1016-1019
pubmed: 31428294
J Med Internet Res. 2020 Jan 30;22(1):e16204
pubmed: 32012066
Clin Infect Dis. 2016 Apr 1;62(7):863-870
pubmed: 26787171
Microbiome. 2019 May 15;7(1):75
pubmed: 31092280
Gastroenterology. 1996 Dec;111(6):1611-20
pubmed: 8942741
J Nutr. 2008 Sep;138(9):1796S-1800S
pubmed: 18716189
Sci Transl Med. 2016 Jun 15;8(343):343ra82
pubmed: 27306664
Cell Chem Biol. 2019 Jan 17;26(1):27-34.e4
pubmed: 30482679
Curr Opin Gastroenterol. 2014 May;30(3):332-8
pubmed: 24625896
J Lipid Res. 1979 Mar;20(3):325-33
pubmed: 36438
Microbiome. 2019 Jan 23;7(1):9
pubmed: 30674356
Front Med (Lausanne). 2017 Oct 03;4:163
pubmed: 29043249
Genome Med. 2016 Apr 13;8(1):39
pubmed: 27074706
Appl Environ Microbiol. 2004 Oct;70(10):5810-7
pubmed: 15466518
J Lipid Res. 2009 Oct;50(10):1955-66
pubmed: 19346330
Sci Transl Med. 2016 Jun 15;8(343):343ra81
pubmed: 27306663
J Biol Chem. 1990 Jul 5;265(19):10925-8
pubmed: 2358447
Anal Chem. 2016 May 3;88(9):4661-8
pubmed: 27065191
Proc Natl Acad Sci U S A. 2012 Jan 31;109(5):1691-6
pubmed: 22307632
Eur Respir J. 2015 Apr;45(4):1055-65
pubmed: 25537565
World J Gastroenterol. 2014 Nov 21;20(43):16079-94
pubmed: 25473159
J Lipid Res. 2014 May;55(5):978-90
pubmed: 24627129
Nat Commun. 2019 Jul 17;10(1):3136
pubmed: 31316056
Front Immunol. 2014 Sep 05;5:427
pubmed: 25250028
Gigascience. 2015 Sep 14;4:42
pubmed: 26380076
Nature. 2019 Oct;574(7776):117-121
pubmed: 31534227
Gut Microbes. 2016 May 3;7(3):201-15
pubmed: 27003186