The SELENOT mimetic PSELT promotes nerve regeneration by increasing axonal myelination in a facial nerve injury model in female rats.

PSELT RRID:AB_2144666 RRID:AB_2340376 RRID:AB_2340607 RRID:AB_2340812 RRID:AB_2811056 RRID:AB_95186 SELENOT axotomy facial nerve motor unit myelin peptide selenoprotein therapy

Journal

Journal of neuroscience research
ISSN: 1097-4547
Titre abrégé: J Neurosci Res
Pays: United States
ID NLM: 7600111

Informations de publication

Date de publication:
09 2022
Historique:
revised: 17 03 2022
received: 13 07 2021
accepted: 06 06 2022
pubmed: 23 6 2022
medline: 22 7 2022
entrez: 22 6 2022
Statut: ppublish

Résumé

Peripheral nerve injury (PNI) is frequent and many patients suffer lifelong disabilities in severe cases. Although the peripheral nervous system is able to regenerate, its potential is limited. In this study, we tested in a nerve regeneration model in rat the potential beneficial effect of a short mimetic peptide, named PSELT, which derives from SELENOT, an essential thioredoxin-like selenoprotein endowed with neuroprotective and antioxidant activities. For this purpose, the right facial nerve of female Long-Evans rats was axotomized then bridged with a free femoral vein interposition graft. PSELT (1 μM) was injected into the vein immediately and 48 h after the injury, and the effects observed were compared to those found after an end-to-end suture used as a gold standard treatment. Whisking behavior, electrophysiological potential, and histological analyses were performed 3 months after injury to determine the effects of these treatments. These analyses revealed that PSELT-treated animals exhibit a better motor recovery in terms of protraction amplitude and velocity of vibrissae compared to control and end-sutured nerve animal groups. Moreover, administration of PSELT following injury enhanced muscle innervation, axonal elongation, and myelination of newly formed nerve fibers. Altogether, these results indicate that a PSELT-based treatment is sufficient to enhance facial nerve myelination and regeneration and could represent a new therapeutic tool to treat PNI.

Identifiants

pubmed: 35730417
doi: 10.1002/jnr.25098
pmc: PMC9545325
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1721-1731

Informations de copyright

© 2022 The Authors. Journal of Neuroscience Research published by Wiley Periodicals LLC.

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Auteurs

Hugo Pothion (H)

Normandie Univ, UNIROUEN, INSERM U1239, Neuroendocrine, Endocrine and Germinal Differentiation and Communication Laboratory, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.
Normandie Univ, UNIROUEN, UR 3830, Groupe de Recherche sur l'Handicap Ventilatoire et Neurologique, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.
Fédération Hospitalo-Universitaire (FHU) Surface, Rouen, France.

Isabelle Lihrmann (I)

Normandie Univ, UNIROUEN, INSERM U1239, Neuroendocrine, Endocrine and Germinal Differentiation and Communication Laboratory, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.

Celia Duclos (C)

Normandie Univ, UNIROUEN, UR 3830, Groupe de Recherche sur l'Handicap Ventilatoire et Neurologique, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.

Gaëtan Riou (G)

Normandie Univ, UNIROUEN, INSERM U1234, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.

Dorthe Cartier (D)

Normandie Univ, UNIROUEN, INSERM U1239, Neuroendocrine, Endocrine and Germinal Differentiation and Communication Laboratory, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.

Loubna Boukhzar (L)

Normandie Univ, UNIROUEN, INSERM U1239, Neuroendocrine, Endocrine and Germinal Differentiation and Communication Laboratory, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.

Benjamin Lefranc (B)

Normandie Univ, UNIROUEN, INSERM U1239, Neuroendocrine, Endocrine and Germinal Differentiation and Communication Laboratory, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.
Normandie Univ, UNIROUEN, UMS-UAR HERACLES, PRIMACEN, Cell Imaging Platform of Normandy, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.

Jérôme Leprince (J)

Normandie Univ, UNIROUEN, INSERM U1239, Neuroendocrine, Endocrine and Germinal Differentiation and Communication Laboratory, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.
Normandie Univ, UNIROUEN, UMS-UAR HERACLES, PRIMACEN, Cell Imaging Platform of Normandy, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.

Nicolas Guérout (N)

Normandie Univ, UNIROUEN, UR 3830, Groupe de Recherche sur l'Handicap Ventilatoire et Neurologique, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.
Fédération Hospitalo-Universitaire (FHU) Surface, Rouen, France.

Jean-Paul Marie (JP)

Normandie Univ, UNIROUEN, UR 3830, Groupe de Recherche sur l'Handicap Ventilatoire et Neurologique, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.
Fédération Hospitalo-Universitaire (FHU) Surface, Rouen, France.
Otorhinolaryngology and Head Neck Surgery Department, Rouen University Hospital, Rouen, France.

Youssef Anouar (Y)

Normandie Univ, UNIROUEN, INSERM U1239, Neuroendocrine, Endocrine and Germinal Differentiation and Communication Laboratory, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.
Fédération Hospitalo-Universitaire (FHU) Surface, Rouen, France.

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