Metformin and vitamin D modulate adipose-derived stem cell differentiation towards the beige phenotype.
Adipose-derived stem cells
adipogenesis
cellular mechanisms
conditioned media
differentiation
gene expression
Journal
Adipocyte
ISSN: 2162-397X
Titre abrégé: Adipocyte
Pays: United States
ID NLM: 101567863
Informations de publication
Date de publication:
12 2022
12 2022
Historique:
entrez:
23
6
2022
pubmed:
24
6
2022
medline:
25
6
2022
Statut:
ppublish
Résumé
Adipose-derived stem cells (ADSCs) represent an ideal stem cell population for regenerative medicine. ADSC adipogenic differentiation is controlled by the activation of a specific transcriptional program, including epigenetic factors and key adipogenic genes. Under certain conditioned media, ADSCs can differentiate into several phenotypes. We previously demonstrated that bioactive molecules could counteract lipid accumulation and regulate adipogenesis, acting on inflammation and vitamin D metabolism. In the present paper, we aimed at evaluating the effect of metformin and vitamin D in targeting ADSC differentiation towards an intermediate phenotype, as beige adipocytes. We exposed ADSCs to different conditioned media and then we evaluated the levels of expression of main markers of adipogenesis, aP2, LPL and ACOT2. We also analysed the gene and protein expression of thermogenic UCP1 protein, and the expression of PARP1 and the beige specific marker TMEM26. Our results showed a novel effect of metformin and vitamin D not only in inhibiting adipogenesis, but also in inducing a specific 'brown-like' phenotype. These findings pave the way for their possible application in the control of
Identifiants
pubmed: 35734882
doi: 10.1080/21623945.2022.2085417
pmc: PMC9235891
doi:
Substances chimiques
Culture Media, Conditioned
0
Vitamin D
1406-16-2
Metformin
9100L32L2N
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
356-365Références
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