Equine Oviductal Organoid Generation and Cryopreservation.
cell culture
cryopreservation
equine
fallopian tube
horse
in vitro
mare
organoid
oviduct
Journal
Methods and protocols
ISSN: 2409-9279
Titre abrégé: Methods Protoc
Pays: Switzerland
ID NLM: 101720073
Informations de publication
Date de publication:
15 Jun 2022
15 Jun 2022
Historique:
received:
03
03
2022
revised:
11
06
2022
accepted:
13
06
2022
entrez:
23
6
2022
pubmed:
24
6
2022
medline:
24
6
2022
Statut:
epublish
Résumé
Organoids are a type of three-dimensional (3D) cell culture that more closely mimic the in vivo environment and can be maintained in the long term. To date, oviductal organoids have only been reported in laboratory mice, women, and cattle. Equine oviductal organoids were generated and cultured for 42 days (including 3 passages and freeze-thawing at passage 1). Consistent with the reports in mouse and human oviductal organoids, the equine oviductal organoids revealed round cell clusters with a central lumen. Developing a 3D model of the mare oviduct may allow for an increased understanding of their normal physiology, including hormonal regulation. These organoids may provide an environment that mimics the in vivo equine oviduct and facilitate improved in vitro embryo production in equids.
Identifiants
pubmed: 35736552
pii: mps5030051
doi: 10.3390/mps5030051
pmc: PMC9230449
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Colorado State University Research Council of the College of Veterinary Medicine and Biomedi-cal Sciences
ID : N/A
Références
Sci Rep. 2020 Jul 28;10(1):12581
pubmed: 32724113
Reproduction. 2016 Dec;152(6):629-644
pubmed: 27601716
Cell Rep. 2020 Feb 4;30(5):1463-1477.e7
pubmed: 32023462
Stem Cell Res. 2018 Oct;32:51-60
pubmed: 30176443
Mol Hum Reprod. 2018 Mar 1;24(3):143-157
pubmed: 29370405
Nat Commun. 2019 Nov 26;10(1):5367
pubmed: 31772167
Science. 2014 Jul 18;345(6194):1247125
pubmed: 25035496
J Extracell Vesicles. 2018 Nov 23;7(1):1535750
pubmed: 30637094
Reproduction. 2016 Dec;152(6):R233-R245
pubmed: 27651517
Cryobiology. 2019 Dec;91:90-96
pubmed: 31626783
Cryobiology. 2022 Feb;104:56-62
pubmed: 34788682
Proc Natl Acad Sci U S A. 2021 Apr 13;118(15):
pubmed: 33876774
Sci Rep. 2020 May 25;10(1):8619
pubmed: 32451384
Development. 2017 May 15;144(10):1775-1786
pubmed: 28442471
Reproduction. 2020 Oct;160(4):613-625
pubmed: 32698140
Int J Mol Sci. 2020 Jul 27;21(15):
pubmed: 32727074
Theriogenology. 2020 Nov;157:210-217
pubmed: 32814248
Theriogenology. 2020 Oct 1;155:240-255
pubmed: 32791377
Nat Commun. 2015 Dec 08;6:8989
pubmed: 26643275
Vet Res. 2021 Mar 10;52(1):42
pubmed: 33691745
Biol Reprod. 2020 Oct 29;103(5):918-926
pubmed: 32697306
Cancers (Basel). 2020 Mar 15;12(3):
pubmed: 32183493
Theriogenology. 2018 Jul 1;114:199-205
pubmed: 29653387
Stem Cell Reports. 2020 Apr 14;14(4):717-729
pubmed: 32243841
Reproduction. 2017 Sep;154(3):153-168
pubmed: 28630101
Nature. 2018 Dec;564(7735):263-267
pubmed: 30487605
Int J Mol Sci. 2022 Mar 16;23(6):
pubmed: 35328607
Int J Mol Sci. 2020 May 25;21(10):
pubmed: 32466321
Reproduction. 2017 Apr;153(4):461-470
pubmed: 28104825
Nat Cell Biol. 2017 May;19(5):568-577
pubmed: 28394884
Reproduction. 2020 Mar 01;159(3):R125-R137
pubmed: 32040278
Nat Med. 2019 May;25(5):838-849
pubmed: 31011202
Reproduction. 2020 Dec;160(6):819-831
pubmed: 33112764
EMBO J. 2020 Mar 16;39(6):e104013
pubmed: 32009247
Reproduction. 2017 Aug;154(2):167-180
pubmed: 28652254
Biotechnol Bioeng. 2021 Mar;118(3):1029-1049
pubmed: 33085083
J Biomed Sci. 2020 Feb 8;27(1):32
pubmed: 32035490
Nat Cell Biol. 2021 Feb;23(2):184-197
pubmed: 33462395