Overview of the Maintenance of Authentic Cancer Cell Lines.
Cancer cell lines
Cell line identification
Cross-contamination
Mycoplasm
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
23
6
2022
pubmed:
24
6
2022
medline:
28
6
2022
Statut:
ppublish
Résumé
The proper maintenance of cancer cell lines is critical to maintain the integrity of any experiment. Proper maintenance includes handling of cell lines and maintaining records on usage and confirmation of identity. Cross-contamination of cancer cell lines has been well documented by the ATCC and must be avoided at all costs. In addition, contamination by Mycoplasma sp. is a well-known issue, which, if left unchecked, can affect all data collected as this contamination can affect cell line metabolism and has uncharacterized effects. Considerations and specific recommendations for the proper maintenance of cancer cell lines are presented here.
Identifiants
pubmed: 35737228
doi: 10.1007/978-1-0716-2376-3_1
doi:
Types de publication
Journal Article
Review
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-7Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Capes-Davis A, Theodosopoulos G, Atkin I et al (2010) Check your cultures! A list of cross-contaminated or misidentified cell lines. Int J Cancer 127:1–8
doi: 10.1002/ijc.25242
Chatterjee R (2007) Cases of mistaken identity. Science 315:928–931
doi: 10.1126/science.315.5814.928
Esquenet M, Swinnen JV, Heyns W et al (1997) LNCaP prostatic adenocarcinoma cells derived from low and high passage numbers display divergent responses not only to androgens but also to retinoids. J Steroid Biochem Mol Biol 62:391–399
doi: 10.1016/S0960-0760(97)00054-X
Drexler HG, Uphoff CC (2002) Mycoplasma contamination of cell cultures: incidence, sources, effects, detection, elimination, prevention. Cytotechnology 39:75–90
doi: 10.1023/A:1022913015916
Yu T, Wang Y, Zhang H et al (2016) Metabolomics reveals mycoplasma contamination interferes with the metabolism of PANC-1 cells. Anal Bioanal Chem 408:4267–4273
doi: 10.1007/s00216-016-9525-9
Feng N, Huang X, Jia Y (2019) Mycoplasma contamination affects cell characteristics and decreases the sensitivity of BV2 microglia to LPS stimulation. Cytotechnology 71:623–634
doi: 10.1007/s10616-019-00311-8
Kagemann G, Henrich B, Kuhn M et al (2005) Impact of mycoplasma hyorhinis infection on L-arginine metabolism: differential regulation of the human and murine iNOS gene. Biol Chem 386:1055–1063
doi: 10.1515/BC.2005.121