Flow Cytometric Analysis of Leukocyte Populations in the Lung Tissue of Dromedary Camels.
camel
flow cytometry
immune cells
lung
mucosal immunology
phagocytosis
Journal
Veterinary sciences
ISSN: 2306-7381
Titre abrégé: Vet Sci
Pays: Switzerland
ID NLM: 101680127
Informations de publication
Date de publication:
10 Jun 2022
10 Jun 2022
Historique:
received:
26
05
2022
revised:
07
06
2022
accepted:
08
06
2022
entrez:
23
6
2022
pubmed:
24
6
2022
medline:
24
6
2022
Statut:
epublish
Résumé
Respiratory tract infections are among the most common infections in dromedary camels, with a high impact on animal health, production, and welfare. Tissue-specific distribution of immune cells is one of the important factors that influence the nature and outcome of the immune response to pathogens. Several protocols have recently been described for the flow cytometric analysis of immune cells in the lung tissue of several species. However, no such protocol currently exists for dromedary camels. The aim of the present study was, therefore, to establish a flow cytometric protocol for the identification of immune cell populations in the camel lung tissue and the evaluation of some of their phenotypic and functional properties. Combined staining of camel lung leukocytes with monoclonal antibodies to the pan-leukocyte marker CD45 and the myeloid cell marker CD172a allowed the identification of myeloid cells (CD45
Identifiants
pubmed: 35737339
pii: vetsci9060287
doi: 10.3390/vetsci9060287
pmc: PMC9231337
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Deanship of Scientific Research, Vice Presidency for Graduate Stud-ies and Scientific Research, King Faisal University, Al-Ahsa, Saudi Arabia
ID : GRANT204
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