Oxidant and Antioxidant Parameters' Assessment Together with Homocysteine and Muscle Enzymes in Racehorses: Evaluation of Positive Effects of Exercise.

aspartate aminotransferase athlete horse creatine kinase endogenous antioxidants exercise lactate dehydrogenase oxidative stress

Journal

Antioxidants (Basel, Switzerland)
ISSN: 2076-3921
Titre abrégé: Antioxidants (Basel)
Pays: Switzerland
ID NLM: 101668981

Informations de publication

Date de publication:
15 Jun 2022
Historique:
received: 24 05 2022
revised: 09 06 2022
accepted: 14 06 2022
entrez: 24 6 2022
pubmed: 25 6 2022
medline: 25 6 2022
Statut: epublish

Résumé

This study aimed to evaluate the changes in serum oxidant and antioxidant parameters together with the serum values of homocysteine (Hcy) and muscle enzymes including creatine kinase (CK), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) in regularly trained athletic horses during official races of 1200, 1600 and 2000 m. Thirty Thoroughbred horses were divided into three groups of 10 subjects each according to the race distance: Group 1, 1200 m race; Group 2, 1600 m race; Group 3, 2000 m race. Blood samples were collected from horses 1 week prior to the race (1WB), on the day of the race at rest (TREST), immediately after the race (TPOST), and after 30 (TPOST30) and 120 (TPOST120) minutes. Serum total proteins, reactive oxygen metabolites (dROMs), thiol antioxidant barrier (SHp), antioxidant barrier (Oxy-ads), Hcy, CK, AST and LDH values were assessed. A two-way repeated measures ANOVA did not show differences referable to the race distance (Group effect) on all investigated parameters (p > 0.05). An exercise effect on oxidative stress markers, Hcy and muscle enzymes herein investigated was found in all groups (p < 0.001). A Pearson’s test showed dROMs positively correlated with SHp, Oxy-ads and Hcy after exercise (p < 0.05). This study suggests that, though well-trained racing horses are subjected to oxidative stress during a race, a proper antioxidant capacity may improve their ability to cope with exercise-induced oxidative stress.

Identifiants

pubmed: 35740073
pii: antiox11061176
doi: 10.3390/antiox11061176
pmc: PMC9220350
pii:
doi:

Types de publication

Journal Article

Langues

eng

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Auteurs

Francesca Arfuso (F)

Department of Veterinary Sciences, University of Messina, 98168 Messina, Italy.

Maria Rizzo (M)

Department of Veterinary Sciences, University of Messina, 98168 Messina, Italy.

Claudia Giannetto (C)

Department of Veterinary Sciences, University of Messina, 98168 Messina, Italy.

Elisabetta Giudice (E)

Department of Veterinary Sciences, University of Messina, 98168 Messina, Italy.

Roberta Cirincione (R)

Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", 90129 Palermo, Italy.

Giovanni Cassata (G)

Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", 90129 Palermo, Italy.

Luca Cicero (L)

Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", 90129 Palermo, Italy.

Giuseppe Piccione (G)

Department of Veterinary Sciences, University of Messina, 98168 Messina, Italy.

Classifications MeSH