Oxidative Stress and Arginine/Nitric Oxide Pathway in Red Blood Cells Derived from Patients with Prediabetes.
eryptosis
nitric oxide pathway
oral glucose tolerance test
prediabetes
red blood cells
Journal
Biomedicines
ISSN: 2227-9059
Titre abrégé: Biomedicines
Pays: Switzerland
ID NLM: 101691304
Informations de publication
Date de publication:
14 Jun 2022
14 Jun 2022
Historique:
received:
16
05
2022
revised:
09
06
2022
accepted:
11
06
2022
entrez:
24
6
2022
pubmed:
25
6
2022
medline:
25
6
2022
Statut:
epublish
Résumé
The effects of the oral glucose tolerance test (OGTT) on red blood cells (RBCs) have not been thoroughly investigated, although it is known that the ingestion of 75 g of glucose during OGTT results in a systemic state of inflammation and oxidative stress. Therefore, we evaluated the effect of OGTT on oxidative stress and L-arginine/Nitric Oxide (L-Arg/NO) metabolic pathway in RBCs obtained from patients with prediabetes. Blood samples were collected from all participants before (T0) and at 10 (T1), 20 (T2), 30 (T3), 60 (T4), 90 (T5), 120 (T6), 150 (T7), and 180 (T8) minutes after glucose loading. Results showed a significant increase in oxidative stress status characterized by a rise in the GSSG/GSH ratio at T4 and T6 that increased in parallel with a reduction of NO production in RBCs. In addition, in this time frame, increased exposure of phosphatidylserine on RBCs membrane was observed. These metabolic modifications were rescued at T8, together with an increase in activated RBC NO synthase expression. These findings provide a possible explanation of the phenomena occurring after glucose loading and suggest that, even in the early stages of diabetes, it may be important to avoid acute variations in glycemia in order to prevent diabetic complications.
Identifiants
pubmed: 35740426
pii: biomedicines10061407
doi: 10.3390/biomedicines10061407
pmc: PMC9219800
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Italian Ministry of Health
ID : RC 2011 ID 184472
Organisme : Italian Ministry of Health
ID : RF CCFM-2008-11153256
Références
Anal Biochem. 2010 Feb 15;397(2):168-74
pubmed: 19825360
Int J Biochem Cell Biol. 2012 Aug;44(8):1236-43
pubmed: 22561748
Blood. 2006 Apr 1;107(7):2943-51
pubmed: 16368881
Molecules. 2017 Jan 21;22(1):
pubmed: 28117726
J Endocrinol Invest. 1997 Sep;20(8):471-5
pubmed: 9364250
Circulation. 2002 Aug 20;106(8):987-92
pubmed: 12186805
PLoS One. 2013 Aug 05;8(8):e66945
pubmed: 23940508
Atherosclerosis. 2011 Sep;218(1):220-5
pubmed: 21632053
Circulation. 2002 Oct 15;106(16):2067-72
pubmed: 12379575
Cardiovasc Res. 2010 Jan 1;85(1):147-54
pubmed: 19726439
Eur J Nutr. 2005 Jun;44(4):250-4
pubmed: 15309420
Clin Hemorheol Microcirc. 2016;62(1):63-9
pubmed: 26410856
Diabetes Care. 2017 Jan;40(Suppl 1):S11-S24
pubmed: 27979889
Redox Biol. 2014 Jan 09;2:251-8
pubmed: 24494200
Biochemistry. 2015 Mar 24;54(11):1989-2000
pubmed: 25751535
J Cataract Refract Surg. 2005 Feb;31(2):369-78
pubmed: 15767161
Am J Ther. 2007 Mar-Apr;14(2):194-202
pubmed: 17414590
Diabetes Care. 2005 Jul;28(7):1786-8
pubmed: 15983335
Prog Lipid Res. 2005 Jul;44(4):207-34
pubmed: 15979148
Nutr Metab Cardiovasc Dis. 2011 Jan;21(1):1-10
pubmed: 21159496
Anal Biochem. 2011 May 1;412(1):108-10
pubmed: 21255552
Endocr Pract. 2008 Oct;14(7):933-46
pubmed: 18996826
Exp Biol Med (Maywood). 2016 Jun;241(12):1323-31
pubmed: 27302176
Diabetologia. 2000 Feb;43(2):156-64
pubmed: 10753036
Circulation. 2009 Feb 17;119(6):812-9
pubmed: 19188510
J Membr Biol. 2010 Jun;235(3):177-89
pubmed: 20526772
Circulation. 2008 Jan 15;117(2):163-168
pubmed: 18172029
Diabetes Care. 1997 Jul;20(7):1183-97
pubmed: 9203460
Diabetes Metab Syndr Obes. 2015 Oct 30;8:525-33
pubmed: 26586957
J Cell Biochem. 2012 May;113(5):1581-9
pubmed: 22174104
Ann Hematol. 2018 Apr;97(4):605-616
pubmed: 29332224
Oncol Lett. 2012 Dec;4(6):1247-1253
pubmed: 23205122
Exp Clin Endocrinol Diabetes. 2008 Feb;116(2):112-7
pubmed: 18286427
Metabolism. 2008 Jan;57(1):110-5
pubmed: 18078867
Diabetes Care. 2022 Jan 1;45(Suppl 1):S17-S38
pubmed: 34964875
Diabetes Care. 1999 Sep;22(9):1490-3
pubmed: 10480514
Acta Diabetol. 2013 Aug;50(4):489-95
pubmed: 21437568
Diabet Med. 2004 Jan;21(1):52-8
pubmed: 14706054
Arch Intern Med. 2001 Feb 12;161(3):397-405
pubmed: 11176766
Front Immunol. 2020 Nov 04;11:551441
pubmed: 33250889
Am J Physiol Heart Circ Physiol. 2003 May;284(5):H1577-84
pubmed: 12521942
Proc Soc Exp Biol Med. 1999 Nov;222(2):178-84
pubmed: 10564543
Diab Vasc Dis Res. 2019 Sep;16(5):458-465
pubmed: 31046456
Diabetes Res Clin Pract. 2019 Dec;158:107911
pubmed: 31707004
Arch Pathol Lab Med. 2009 Apr;133(4):628-32
pubmed: 19391664
Eur J Clin Invest. 1998 Apr;28(4):329-33
pubmed: 9615913
Transfusion. 2013 May;53(5):981-9
pubmed: 22897637
Cell Death Differ. 2003 Feb;10(2):249-56
pubmed: 12700653
Pflugers Arch. 2008 May;456(2):293-305
pubmed: 18058124
J Thromb Haemost. 2010 Aug;8(8):1663-9
pubmed: 20492456
Am J Cardiol. 2010 Oct 1;106(7):988-93
pubmed: 20854962
Am J Epidemiol. 2010 Jan 15;171(2):214-20
pubmed: 20008450
Am J Physiol Cell Physiol. 2006 Jan;290(1):C244-53
pubmed: 16338977
Free Radic Res. 2015 May;49(5):633-49
pubmed: 25511471
Transfusion. 2021 Feb;61(2):435-448
pubmed: 33146433
Lancet. 2012 Jun 16;379(9833):2279-90
pubmed: 22683128
Cell Physiol Biochem. 2005;16(1-3):1-8
pubmed: 16121027
JAMA. 2005 Jul 6;294(1):81-90
pubmed: 15998894
J Pharm Biomed Anal. 2012 Dec;71:111-8
pubmed: 22947504
Nitric Oxide. 2001 Aug;5(4):317-33
pubmed: 11485370
Eur J Clin Invest. 2011 May;41(5):465-73
pubmed: 21128931
Cell Physiol Biochem. 2005;16(4-6):133-46
pubmed: 16301814
Exp Biol Med (Maywood). 2021 Jan;246(2):153-162
pubmed: 32957808
Am J Physiol Cell Physiol. 2012 Feb 15;302(4):C644-51
pubmed: 22173866
Mol Cell Biochem. 2011 Nov;357(1-2):171-9
pubmed: 21625956
J Diabetes Res. 2013;2013:849295
pubmed: 23691521
Diabetologia. 2006 May;49(5):822-7
pubmed: 16525842
Cell Physiol Biochem. 2010;26(1):21-8
pubmed: 20502001
Antioxid Redox Signal. 2014 Jul 1;21(1):138-53
pubmed: 24359125
J Clin Endocrinol Metab. 2006 Mar;91(3):813-9
pubmed: 16352690
Free Radic Res. 2011 Jun;45(6):653-61
pubmed: 21480762
Diabetes. 2003 Jun;52(6):1475-84
pubmed: 12765960
Circulation. 1997 Apr 1;95(7):1783-90
pubmed: 9107164