Targeted Analysis of HSP70 Isoforms in Human Spermatozoa in the Context of Capacitation and Motility.
HSP70 isoforms
LC–MRM mass spectrometry
capacitation
sperm motility
spermatozoa
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
10 Jun 2022
10 Jun 2022
Historique:
received:
14
05
2022
revised:
07
06
2022
accepted:
08
06
2022
entrez:
24
6
2022
pubmed:
25
6
2022
medline:
28
6
2022
Statut:
epublish
Résumé
HSP70s constitute a family of chaperones, some isoforms of which appear to play a role in sperm function. Notably, global proteomic studies analyzing proteins deregulated in asthenozoospermia, a main cause of male infertility characterized by low sperm motility, showed the dysregulation of some HSP70 isoforms. However, to date, no clear trend has been established since the variations in the abundance of HSP70 isoforms differed between studies. The HSPA2 isoform has been reported to play a key role in fertilization, but its dysregulation and possible relocation during capacitation, a maturation process making the spermatozoon capable of fertilizing an oocyte, is debated in the literature. The aim of the present study was to investigate the fate of all sperm HSP70 isoforms during capacitation and in relation to sperm motility. Using Multiple-Reaction Monitoring (MRM) mass spectrometry, we showed that the relative abundance of all detected isoforms was stable between non-capacitated and capacitated spermatozoa. Immunofluorescence using two different antibodies also demonstrated the stability of HSP70 isoform localization during capacitation. We also investigated spermatozoa purified from 20 sperm samples displaying various levels of total and progressive sperm motility. We showed that the abundance of HSP70 isoforms is not correlated to sperm total or progressive motility.
Identifiants
pubmed: 35742939
pii: ijms23126497
doi: 10.3390/ijms23126497
pmc: PMC9224233
pii:
doi:
Substances chimiques
HSP70 Heat-Shock Proteins
0
Protein Isoforms
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Fund for Medical Research in Hainaut (F.R.M.H.)
ID : Not available
Organisme : UMons Research Institute for Biosciences
ID : HSP70 project
Organisme : Walloon Government
ID : Bioprofiling platform
Organisme : European Regional Development Fund
ID : Bioprofiling platform
Organisme : UMHAP (UMons / Hôpital Ambroise Paré) Medical Research Centre - Scientific Inspiration for Medical Excellence in Mons
ID : doctoral grant Marie Bisconti
Organisme : Fund for Scientific Research
ID : Post-doctoral grant Baptiste Martinet
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